Recellularization of Rat Liver Scaffolds by Human Liver Stem Cells

Navarro-Tableros, Víctor; Sanchez, Maria Beatriz Herrera; Figliolini, Federico; Romagnoli, Renato; Tetta, Ciro; Camussi, Giovanni

doi:10.1089/ten.tea.2014.0573

Cited by 61 publications

(50 citation statements)

References 39 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…For example, seeding rat hepatocytes into decellularized liver and subsequent transplantation in rats showed that less damages occurred to the cells following an 8 h transplantation, as revealed by TUNEL staining . In a separate study, when human liver stem cells were seeded in decellularized rat liver, differentiation into functional hepatocytes was observed as well as the presence of epithelial and endothelial‐like cells reported . Research on achieving human‐sized whole liver has also been attempted with pigs by decellularizing the organ with SDS (sodium dodecyl sulfate) …”

Section: Major Tissues and Organs Used For Decellularizationmentioning

confidence: 99%

“…9 In a separate study, when human liver stem cells were seeded in decellularized rat liver, differentiation into functional hepatocytes was observed as well as the presence of epithelial and endothelial-like cells reported. 12 Research on achieving human-sized whole liver has also been attempted with pigs by decellularizing the organ with SDS (sodium dodecyl sulfate). 13,14 Heart Heart decellularization techniques started initially with heart valves by surface decellularization using detergents and subsequent recellularization with different cell types.…”

Section: Major Tissues and Organs Used For Decellularizationmentioning

confidence: 99%

See 1 more Smart Citation

Tissue and organ decellularization in regenerative medicine

Damodaran

Vermette

2018

Biotechnology Progress

View full text Add to dashboard Cite

The advancement and improvement in decellularization methods can be attributed to the increasing demand for tissues and organs for transplantation. Decellularized tissues and organs, which are free of cells and genetic materials while retaining the complex ultrastructure of the extracellular matrix (ECM), can serve as scaffolds to subsequently embed cells for transplantation. They have the potential to mimic the native physiology of the targeted anatomic site. ECM from different tissues and organs harvested from various sources have been applied. Many techniques are currently involved in the decellularization process, which come along with their own advantages and disadvantages. This review focuses on recent developments in decellularization methods, the importance and nature of detergents used for decellularization, as well as on the role of the ECM either as merely a physical support or as a scaffold in retaining and providing cues for cell survival, differentiation and homeostasis. In addition, application, status, and perspectives on commercialization of bioproducts derived from decellularized tissues and organs are addressed. © 2018 American Institute of Chemical Engineers Biotechnol. Prog., 34:1494–1505, 2018

show abstract

Section: Major Tissues and Organs Used For Decellularizationmentioning

confidence: 99%

Section: Major Tissues and Organs Used For Decellularizationmentioning

confidence: 99%

Tissue and organ decellularization in regenerative medicine

Damodaran

Vermette

2018

Biotechnology Progress

View full text Add to dashboard Cite

show abstract

“…A disinfection step is commonly applied before the terminal sterilization process. Peracetic acid (PAA) is the most commonly used disinfectant during biological scaffold decellularization . It achieves disinfection by oxidizing the cell membrane of microorganisms.…”

Section: Whole Liver Decellularizationmentioning

confidence: 99%

“…Moreover, mature hepatocyte markers were observed by 21 days and the addition of growth factors reduced the percentage of vimentin + cells. Interestingly, the albumin + cells were also found to form a continuous layer lining the lumen structure in the scaffold …”

Section: Liver Scaffold Recellularizationmentioning

confidence: 99%

Whole liver engineering: A promising approach to develop functional liver surrogates

Meng

Assiri

Dhar

et al. 2017

Liver International

View full text Add to dashboard Cite

Liver donor shortage remains the biggest challenge for patients with end-stage liver failures. While bioartificial liver devices have been developed as temporary supports for patients waiting for transplantation, their applications have been limited clinically.Whole liver engineering is a biological scaffold based regenerative medicine approach that holds promise for developing functional liver surrogates. Significant advancements have been made since the first report in 2010. This review focuses on the recent achievements of whole liver engineering studies. K E Y W O R D Sdecellularization, endothelialization, recellularization, transplantation, whole liver engineering | INTRODUCTIONLiver is the largest internal organ responsible for important physiological functions, including vitamin storage, protein synthesis and detoxification. For patients with end-stage liver failures, that are often associated with hepatitis, bile duct-related diseases or liver cancers, liver transplantation has become the golden treatment. It provides better quality of life and cost effectiveness. The 5-year survival rate following liver transplantation is above 70%. However, the number of patients who needs a transplant still exceeds available donors. In the USA alone, it was reported that more than 16 000 patients need transplantation yearly but only 6000 liver transplantations are performed due to shortage of suitable donors.1 According to US Department of Health and Human Services, the shortage of eligible donors results in 18 deaths every day. There is a need for innovative approaches to develop functional liver surrogates for end-stage liver failure patients.Bioartificial liver devices (BAL) have been developed to temporarily function as surrogates for patients waiting for liver transplants. The BAL systems are extracorporeal, which consist of an artificial component (ie, the bioreactor) and a biological component (ie, hepatocytes).BAL systems have significantly prolonged the animal survival rate in preclinical studies and also have been applied in clinical trials. 2,3However, currently there are no strong evidence supporting the survival benefits of patients utilizing these devices and the incapability to prolong hepatocyte functions has limited their applications clinically. Figure 1 shows the concept of biological scaffold based whole liver engineering. | BioreactorBioreactors are designed to mimic the in vivo physiological environment such that an optimal growth condition is created for cells to repopulate the whole organ scaffolds. Several key components are required to build an appropriate bioreactor, including nutrient/waste exchange, temperature control, gas supply, real time monitoring of biomolecules of interest and organ-type specific stimuli. For example, the presence of air-liquid interface has been shown to induce ciliogenesis in tracheal regeneration 39 and physical stimuli such as ventilation were found beneficial to recellularized lung scaffold culture. 37Identifying specific stimuli that facilitate liver...

show abstract

“…They can express some mesenchymal stem cell markers as well as albumin (ALB), alpha-fetoprotein, and cytokeratin 18 (CK18), suggestive of their partial hepatic commitment [Herrera et al, 2006]. Furthermore, recellularization of a decellularized ECM conducted by the same group induced the generation of functional epithelial-like and endothelial-like cells [Navarro-Tableros et al, 2015].…”

Section: Allogenic Cellsmentioning

confidence: 99%

Recent Advances in Decellularization and Recellularization for Tissue-Engineered Liver Grafts

Wang

Nicolas²,

Chen³

et al. 2016

Cells Tissues Organs

View full text Add to dashboard Cite

Liver transplantation from deceased or living human donors remains the only proven option for patients with end-stage liver disease. However, the shortage of donor organs is a significant clinical concern that has led to the pursuit of tissue-engineered liver grafts generated from decellularized liver extracellular matrix and functional cells. Investigative efforts on optimizing both liver decellularization and recellularization protocols have been made in recent decades. In the current review, we briefly summarize these advances, including the generation of high-quality liver extracellular matrix scaffolds, evaluation criteria for quality control, modification of matrix for enhanced properties, and reseeding strategies. These efforts to optimize the methods of decellularization and recellularization lay the groundwork towards generating a transplantable, human-sized liver graft for the treatment of patients with severe liver disease.

show abstract

Recellularization of Rat Liver Scaffolds by Human Liver Stem Cells

Cited by 61 publications

References 39 publications

Tissue and organ decellularization in regenerative medicine

Tissue and organ decellularization in regenerative medicine

Whole liver engineering: A promising approach to develop functional liver surrogates

Recent Advances in Decellularization and Recellularization for Tissue-Engineered Liver Grafts

Contact Info

Product

Resources

About