Rec. ST6Gal-I variants to control enzymatic activity in processes of in vitro glycoengineering

All-in-one assay for β-d-galactoside sialyltransferases: Quantification of productive turnover, error hydrolysis, and site selectivity

“…For example, sialyltransferases serve as biomarkers in clinical biochemistry [2]. Recently, also the demand for sialyltransferases applicable in biocatalysis, natural product synthesis, and modification of biotherapeutics has rapidly increased in biotechnological markets [3][4][5][6].…”

“…For example, sialyltransferases serve as biomarkers in clinical biochemistry [2]. Recently, also the demand for sialyltransferases applicable in biocatalysis, natural product synthesis, and modification of biotherapeutics has rapidly increased in biotechnological markets [3][4][5][6].Sialyltransferases (EC 2.4.99.X) are glycosyltransferases, which transfer a sialic acid from an activated sugar donor, usually CMP-Neu5Ac (cytidine-5'-monophospho-b-D-N-acetylneuraminic acid) 1 , to a carbohydrate acceptor molecule. These carbohydrates can be free or attached to a protein or lipid [1,7-10].…”