Rec A disruption unveils cross talk between DNA repair and membrane damage, efflux pump activity, biofilm formation in Mycobacterium smegmatis

Hans, Sandeep; Purkait, Dyuti; Nandan, Shiv; Bansal, Maghav; Hameed, Saif; Fatima, Zeeshan

doi:10.1016/j.micpath.2020.104262

Cited by 3 publications

(2 citation statements)

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“…Our results were in line with previous study findings that electric field treatment enhanced the production of ROS [ 10 ]. The high concentration of ROS might hinder the function of some organelles within the cells, thus leading to the destruction of the cell structure [ 34 ]. Furthermore, excessive intracellular ROS could induce the release of apoptotic factors from the mitochondria, causing DNA damage [ 35 ].…”

Section: Resultsmentioning

confidence: 99%

In Vitro Antibacterial Mechanism of High-Voltage Electrostatic Field against Acinetobacter johnsonii

Huang

Gao

Qian

et al. 2022

Foods

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This study aimed to investigate the antibacterial properties and mechanisms of a high-voltage static electric field (HVEF) in Acinetobacter johnsonii, which were assessed from the perspective of biochemical properties and stress-related genes. The time/voltage-kill assays and growth curves showed that an HVEF decreased the number of bacteria and OD600 values. In addition, HVEF treatment caused the leakage of cell contents (nucleic acids and proteins), increased the electrical conductivity and amounts of reactive oxygen substances (ROS) (16.88 fold), and decreased the activity of Na+ K+-ATPase in A. johnsonii. Moreover, the changes in the expression levels of genes involved in oxidative stress and DNA damage in the treated A. johnsonii cells suggested that HVEF treatment could induce oxidative stress and DNA sub-damage. This study will provide useful information for the development and application of an HVEF in food safety.

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Section: Resultsmentioning

confidence: 99%

In Vitro Antibacterial Mechanism of High-Voltage Electrostatic Field against Acinetobacter johnsonii

Huang

Gao

Qian

et al. 2022

Foods

View full text Add to dashboard Cite

show abstract

“…RecA, a RecFOR pathway product, initiates the exchange between the damaged and homologous DNA strands with further help from the RuvABC (a helicase) and RecG (an endonuclease) complexes (Singh et al, 2016). A recent study established a link between DNA repair, drug efflux, and biofilm formation and validated RecA as an effective drug target in Mtb and Msm (Hans et al, 2020). Further studies are required to research the endonuclease involved in HR pathways.…”

Section: Double-strand Breaks (Dsbs) Repairmentioning

confidence: 99%