Rebooting the genome: The role of negative feedback in horizontal gene transfer

Fernández-López, Raúl; Cruz, Fernando de la

doi:10.4161/2159256x.2014.988069

Cited by 11 publications

(10 citation statements)

References 26 publications

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“…Although unusual, the presence of the mphA gene cluster in M. morganii is of little clinical significance, because Enterobacteriaceae are intrinsically resistant to macrolides due to the presence of efflux transporters (Leclercq, 2002 ; Poole et al, 2006 ). However, considering that the human gut is prone to the occurrence of increased horizontal gene transfer between bacteria, mphA gene might get transferred to commensal Gram positive bacteria (Stecher et al, 2012 ); this and the supplementary array of plasmids and prophages harbored by integrons-positive isolates reinforced the potential concerted contribution of different mobile genetic elements to the mobilization and spread of acquired resistance (Fernandez-Lopez and De La Cruz, 2014 ).…”

Section: Discussionmentioning

confidence: 99%

Architecture of Class 1, 2, and 3 Integrons from Gram Negative Bacteria Recovered among Fruits and Vegetables

et al. 2016

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The spread of antibiotic resistant bacteria throughout the food chain constitutes a public health concern. To understand the contribution of fresh produce in shaping antibiotic resistance bacteria and integron prevalence in the food chain, 333 antibiotic resistance Gram negative isolates were collected from organic and conventionally produced fruits (pears, apples, and strawberries) and vegetables (lettuces, tomatoes, and carrots). Although low levels of resistance have been detected, the bacterial genera identified in the assessed fresh produce are often described not only as environmental, but mostly as commensals and opportunistic pathogens. The genomic characterization of integron-harboring isolates revealed a high number of mobile genetic elements and clinically relevant antibiotic resistance genes, of which we highlight the presence of as mcr-1, qnrA1, blaGES−11, mphA, and oqxAB. The study of class 1 (n = 8), class 2 (n = 3) and class 3 (n = 1) integrons, harbored by species such as Morganella morganii, Escherichia coli, Klebsiella pneumoniae, led to the identification of different integron promoters (PcW, PcH1, PcS, and PcWTNG−10) and cassette arrays (containing drfA, aadA, cmlA, estX, sat, and blaGES). In fact, the diverse integron backbones were associated with transposable elements (e.g., Tn402, Tn7, ISCR1, Tn2*, IS26, IS1326, and IS3) that conferred greater mobility. This is also the first appearance of In1258, In1259, and In3-13, which should be monitored to prevent their establishment as successfully dispersed mobile resistance integrons. These results underscore the growing concern about the dissemination of acquired resistance genes by mobile elements in the food chain.

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Section: Discussionmentioning

confidence: 99%

Architecture of Class 1, 2, and 3 Integrons from Gram Negative Bacteria Recovered among Fruits and Vegetables

et al. 2016

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“…W plasmids are the smallest conjugative plasmids found in the Enterobacteriaceae, and most of the few available sequenced examples show a conserved backbone consisting of typical plasmid modules with different insertions (279,280). W plasmids include a master regulation system similar to that of P plasmids (281). A new IncW␤ group was recently suggested for a related plasmid, but it has a different replication module (254).…”

Section: Resistance Plasmids In the Enterobacteriaceaementioning

confidence: 99%

Mobile Genetic Elements Associated with Antimicrobial Resistance

et al. 2018

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SUMMARYStrains of bacteria resistant to antibiotics, particularly those that are multiresistant, are an increasing major health care problem around the world. It is now abundantly clear that both Gram-negative and Gram-positive bacteria are able to meet the evolutionary challenge of combating antimicrobial chemotherapy, often by acquiring preexisting resistance determinants from the bacterial gene pool. This is achieved through the concerted activities of mobile genetic elements able to move within or between DNA molecules, which include insertion sequences, transposons, and gene cassettes/integrons, and those that are able to transfer between bacterial cells, such as plasmids and integrative conjugative elements. Together these elements play a central role in facilitating horizontal genetic exchange and therefore promote the acquisition and spread of resistance genes. This review aims to outline the characteristics of the major types of mobile genetic elements involved in acquisition and spread of antibiotic resistance in both Gram-negative and Gram-positive bacteria, focusing on the so-called ESKAPEE group of organisms (, ,, ,, spp., and), which have become the most problematic hospital pathogens.

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“…Being a core gene does not necessarily mean that the gene positively contributes to plasmid maintenance in particular hosts, but suggests that the gene sets have co-evolved together since the divergence from the most recent common ancestor. The long-term co-evolution of core genes can result in the formation of an operon with a coordinated regulatory system that balances the efficiency of horizontal and vertical transmissions [ [20] , [21] , [22] ]. These core genes may be linked together upon construction of a vector.…”

Section: Key Factors In Plasmid Designmentioning

confidence: 99%

Reconsidering plasmid maintenance factors for computational plasmid design

Yano

Shintani

Tomita

et al. 2019

Computational and Structural Biotechnology Journal

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Plasmids are genetic parasites of microorganisms. The genomes of naturally occurring plasmids are expected to be polished via natural selection to achieve long-term persistence in the microbial cell population. However, plasmid genomes are extremely diverse, and the rules governing plasmid genomes are not fully understood. Therefore, computationally designing plasmid genomes optimized for model and nonmodel organisms remains challenging. Here, we summarize current knowledge of the plasmid genome organization and the factors that can affect plasmid persistence, with the aim of constructing synthetic plasmids for use in gram-negative bacteria. Then, we introduce publicly available resources, plasmid data, and bioinformatics tools that are useful for computational plasmid design.

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Rebooting the genome: The role of negative feedback in horizontal gene transfer

Cited by 11 publications

References 26 publications

Architecture of Class 1, 2, and 3 Integrons from Gram Negative Bacteria Recovered among Fruits and Vegetables

Architecture of Class 1, 2, and 3 Integrons from Gram Negative Bacteria Recovered among Fruits and Vegetables

Mobile Genetic Elements Associated with Antimicrobial Resistance

Reconsidering plasmid maintenance factors for computational plasmid design

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