Reassessment of the phylogenetic relationships of Thiomonas cuprina

Abstract

The published sequence of the 16S rRNA gene of Thiomonas cuprina strain Hö 5 (5DSM 5495 T ) (GenBank accession no. U67162) was found to be erroneous. The 16S rRNA genes from the type strain held by the DSMZ since 1990 (DSM 5495 T 5NBRC 102145 T ) and strain Hö 5 maintained frozen in the Universitä t Regensburg for 23 years (5NBRC 102094) were sequenced and found to be identical, but to show no significant similarity to the U67162 sequence. This also casts some doubt on the previously published 5S and 23S rRNA gene sequences (GenBank accession nos U67171 and X75567). The correct 16S rRNA gene sequence showed 99.8 % identity to those from Thiomonas delicata NBRC 14566 T and 'Thiomonas arsenivorans' DSM 16361. The properties of these three species are re-evaluated, and emended descriptions are provided for the genus Thiomonas and the species Thiomonas cuprina.

Thiomonas bhubaneswarensis sp. nov., an obligately mixotrophic, moderately thermophilic, thiosulfate-oxidizing bacterium

“…A number of thermophilic sulfur-oxidizing betaproteobacteria have been described, including members of genera such as Thiomonas (Shooner et al, 1996;Katayama et al 2006;Kelly et al, 2007), Thermothrix (Odintsova et al, 1996), Tepidimonas (Moreira et al, 2000) and Thiobacillus (Wood & Kelly, 1988;. These organisms have been isolated from terrestrial hot-spring environments or wastewater-treatment plants and most of them are facultative autotrophs or strict heterotrophs.…”

“…Comparative features of strain S10 T and related strains Strains: 1, S10 T ; 2, Thiomonas perometabolis ATCC 23370 T ; 3, Thiomonas intermedia ATCC 15466 T ; 4, Thiomonas cuprina DSM 5495 T ; 5, Thiomonas thermosulfata ATCC 51520 T . Data for reference strains are from Katayama-Fujimura et al (1984), Huber & Stetter (1990), Moreira & Amils (1997), and Kelly et al (2007). ND, No data; +, positive; 2, negative, W, weakly positive.…”

Patterns of Microbial Genetic Diversity and the Correlation Between Bacterial Demographic History and Geohistory

“…The optimal temperature for the growth of Thiomonas spp. ranges from 20°C to 53ºC (estimated from six Thiomonas species) but they can adapt to a wide range of temperatures (some strains can slowly grow at less than 4ºC and up to 65ºC) (Kelly et al, 2007). Although the temperature records of Mt.…”

“…The detailed metabolic properties of Thiomonas spp. were described by Kelly et al (2007). Niunai Lake is a sulfate pond in a stratum of volcano.…”

“…All sequences were tested for possible chimeric artifacts using the Bellerophon software (Kelly et al, 2007). Putative chimeras were excluded from further analyses.…”