Reassessment of the Four Yield-related Genes Gn1a, DEP1, GS3, and IPA1 in Rice Using a CRISPR/Cas9 System

Li, Meiru; Li, Xiaoxia; Zhou, Zejiao; Wu, Pingzhi; Fang, Maichun; Pan, Xiaoping; Lin, Qiupeng; Luo, Wanbin; Wu, Guojiang; Li, Hongqing

doi:10.3389/fpls.2016.00377

Cited by 319 publications

(265 citation statements)

References 59 publications

(117 reference statements)

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“…Rice is the most frequently used crop species for testing and applying CRISPR‐Cas9 tools. Several agronomic traits of rice, including yield, fertility, architecture, biotic and abiotic stress response, and herbicide tolerance, have been successfully modified by targeted mutations in one or multiple major genes (Ikeda et al ., 2016; Li et al ., 2016a,b,c; Osakabe et al ., 2016; Sun et al ., 2016; Wang et al ., 2016; Xu et al ., 2014, 2016; Zhou et al ., 2015). …”

Section: Introductionmentioning

confidence: 99%

Generation of targeted mutant rice using a CRISPR‐Cpf1 system

Qin

et al. 2017

Plant Biotechnology Journal

228

152

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Summary CRISPR‐Cpf1 is a newly identified CRISPR‐Cas system, and Cpf1 was recently engineered as a molecular tool for targeted genome editing in mammalian cells. To test whether the engineered CRISPR‐Cpf1 system could induce the production of rice mutants, we selected two genome targets in the OsPDS and OsBEL genes. Our results show that both targets could be efficiently mutated in transgenic rice plants using CRISPR‐Cpf1. We found that pre‐crRNAs with a full‐length direct repeat sequence exhibited considerably increased efficiencies compared with mature crRNAs. In addition, the specificity and transmission of the mutation were investigated, and the behaviours of crRNA‐Cpf1‐induced plant targeted genome mutagenesis were assessed. Taken together, our results indicate that CRISPR‐Cpf1 expression via stable transformation can efficiently generate specific and heritable targeted mutations in rice and thereby constitutes a novel and important approach to specific and precise plant genome editing.

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Section: Introductionmentioning

confidence: 99%

Generation of targeted mutant rice using a CRISPR‐Cpf1 system

Qin

et al. 2017

Plant Biotechnology Journal

228

152

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“…Например, у риса и пшеницы удалось добиться снижения высоты растения за счет нокаута по гену DEP1 (Li et al, 2016b;Zhang et al, 2016).…”

Section: Plant Genetics Vavilov Journal Of Genetics and Breeding • 21unclassified

Crop genes modified using CRISPR/Cas system

Korotkova¹,

Gerasimova²,

Шумный³

et al. 2017

Vestn. VOGiS

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The CRISPR/Cas system is the most promising among genome editing tools. It can provide the development of modified nontransgenic plants with the possibility of simultaneous multiple targeted mutations. The purpose of this review is to analyze published papers describing the utilization of the CRISPR/Cas system for crop gene modification in order to assess the potential of this technology as a new plant breeding technique. The search for "CRISPR & crop name" within article titles, abstracts and keywords in the Scopus database was carried out for 45 crops. Among a total of 206 search results, only 88 have been recognized as original articles describing editing crop genes with the CRISPR/Cas system. A total of 145 target genes of 15 crops are described in these 88 articles, including rice with the largest number of genes modified (78 genes). In these studies, the ability to get transgene-free modified plants was widely demonstrated. However, in most cases research was aimed at the approbation of the technology or was to elucidate target gene function, while modification of just 37 target genes was related with crop improvement. We present here a catalogue of these genes. In most of these cases, modifications resulted in knockout of the genes such as negative growth and development regulators or negative regulators of plant resistance. In most cases, the phenotype of modified plants was assessed, and the presence of desired changes was shown. However, since the estimated number of "negative regulators" is limited in plant genomes, the CRISPR-directed gene knockout has a restricted potential for crop improvement. Intensive application of the CRISPR/Cas system for more complicate modifications such as replacement of defect alleles by functional ones or insertion of a desired gene is required (so far reports about such modifications are very rare in crops). In addition, to provide a basis for broad practical application of CRISPR/Cas-based genome editing, more cultivars of crop species should be involved in ongoing studies. Just a few genotypes of crop species have been used for gene modifications thus far. Nevertheless, in spite of the restrictions mentioned, essential success has Система CRISPR/Cas -один из самых перспективных способов геномного редактирования. Этот доступный метод позволяет получать нетрансгенные растения с заданными модификациями, причем можно одновременно производить мутации в несколь-ких мишенях. Цель настоящего обзора -анализ опубликованных работ, в которых система CRISPR/Cas использована для модифика-ции генов сельскохозяйственных растений, с тем чтобы оценить потенциал этой технологии как нового метода селекции рас-тений. Для 45 сельскохозяйственных культур проведен поиск по сочетанию ключевого слова CRISPR с названием культуры (поиск осуществлялся в названиях, аннотациях и ключевых словах статей из журналов, индексируемых в базе данных Scopus). Среди 206 результатов поиска только 88 содержали описание эксперимен-тальных работ, в которых использована система CRISPR/Cas. В этих работах описаны ...

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“…Unique features (tolerates DNA methylation and different from ZFN and TALEN) and high efficiency of CRISPR/Cas system gives leverage for genome manipulation in plants such as rice (Ma et al, 2015). In rice and wheat, sequence specific genome modification has been induced by Cas9: sgRNA (Shan et al, 2013;Li et al, 2016). The Arabidopsis, tobacco and sorghum genome modifications also achieved by CRISPR/Cas system .…”

Section: Introductionmentioning

confidence: 99%

Disruption of Phytoene Desaturase Gene using Transient Expression of Cas9: gRNA Complex

Mubarik¹,

Khan

Ahmad³

et al. 2016

IJAB

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Engineered nucleases have emerged as a powerful tool for site specific gene manipulation in plants. Based on Clustered Regularly Interspersed Short Palindromic Repeats/CRISPR associated (CRISPR/Cas) system, engineered Cas9:gRNA complex can be used to cleave specific DNA sequences in the genome. In the present study, Nicotiana benthamiana Phytoene Desaturase (NbPDS) gene was targeted by CRISPR/Cas9 system. The plant codon optimized (pcoCas9) along with guided RNA (gRNA) was cloned in plant expression vector pGreen0029, to target NbPDS gene. The NbPDS gene was disrupted transiently by agroinfiltration of pcoCas9-gRNA complex. Visible albino spots were observed on agro-infiltrated leaves of N. benthamiana plants after 7 days of infiltration. The observed albino spots were analyzed through PCR amplification of gRNAtarget, fluorescent microscopy and chlorophyll contents measurements. Our results support the notion that CRISPR/Cas9 system is a swift, robust and useful tool for targeted gene disruption, deletion and editing.

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Reassessment of the Four Yield-related Genes Gn1a, DEP1, GS3, and IPA1 in Rice Using a CRISPR/Cas9 System

Cited by 319 publications

References 59 publications

Generation of targeted mutant rice using a CRISPR‐Cpf1 system

Generation of targeted mutant rice using a CRISPR‐Cpf1 system

Crop genes modified using CRISPR/Cas system

Disruption of Phytoene Desaturase Gene using Transient Expression of Cas9: gRNA Complex

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