Rearing of Trilobed Male Uncus Ostrinia Species in Laboratory for Experimental Purposes

Фролов, А. Н.; Berim, M. N.; Grushevaya, I. V.

doi:10.31993/2308-6459-2019-3(101)-58-62

Cited by 8 publications

(10 citation statements)

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“…Nosema pyrausta [ 21 ] was propagated in a temporary laboratory culture of the European corn borer, Ostrinia nubilalis Hbn, which is the typical host of this parasite. The larvae were maintained on an artificial diet [ 22 ] and infected with N. pyrausta spores according to Tokarev et al [ 15 ]. To isolate the parasite spores, infected insects were dissected, adipose tissue was homogenized using plastic pestles and centrifuge tubes in distilled water and spun at 500–1000 g for 5–10 min.…”

Section: Methodsmentioning

confidence: 99%

Susceptibility of the Gypsy Moth Lymantria dispar (Lepidoptera: Erebidae) to Nosema pyrausta (Microsporidia: Nosematidae)

Kononchuk

Martemyanov

Ignatieva

et al. 2021

Insects

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The gypsy moth, Lymantria dispar, is a notorious forest defoliator, and various pathogens are known to act as natural regulators of its population density. As a widespread herbivore with a broad range of inhabited areas and host plants, it is potentially exposed to parasitic microorganisms from other insect hosts. In the present paper, we determined the susceptibility of gypsy moth larvae to the microsporidium Nosema pyrausta from the European corn borer, Ostrinia nubilalis. Gypsy moth samples from two localities of Western Siberia were used. N. pyrausta developed infections in the salivary gland and adipose tissue of gypsy moth prepupae and pupae, forming spore masses after 30 days of alimentary exposure to the second instar larvae. Among the experimental groups, the infection levels ranged from 0 to 9.5%. Effects of a covert baculovirus infection, phenylthiourea pretreatment and feeding insects on an artificial diet versus natural foliage were not significant in terms of microsporidia prevalence levels. Thus, L. dispar showed a low level of susceptibility to a non-specific microsporidium. It can be referred to as a resistant model host and not an appropriate substitute host for laboratory propagation of the microsporidium.

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Section: Methodsmentioning

confidence: 99%

Susceptibility of the Gypsy Moth Lymantria dispar (Lepidoptera: Erebidae) to Nosema pyrausta (Microsporidia: Nosematidae)

Kononchuk

Martemyanov

Ignatieva

et al. 2021

Insects

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“…After oviposition, adult moths were checked for microsporidian infection using light microscopy (see below) to exclude naturally infected parents (infected eggs discarded) in order to obtain a microsporidia‐free colony of the host. Hatched larvae were routinely reared on an artificial diet (Frolov et al., 2019).…”

Section: Methodsmentioning

confidence: 99%

Transovarial transmission of Nosema pyrausta in three generations of Ostrinia nubilalis in laboratory tests

Grushevaya

Senderskiy

Zubarev

et al. 2021

Entomologia Exp Applicata

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The microsporidium Nosema pyrausta (Paillot) (Nosematidae) is a widespread and persistent entomopathogen with integrated pest management implications. Nosema pyrausta is capable of horizontal and vertical transmissions in Ostrinia nubilalis (Hübner) (Lepidoptera: Crambidae) populations. After experimental per os infection, N. pyrausta virulence decreased with the instar of the host larvae used. At a dosage of 100,000 spores per fourth instar, 47% pupae and 36% adults survived and gave rise to transovarially infected offspring. The infection persisted through three laboratory generations of the host with prevalence levels ranging between 29 and 92%. Mature microsporidian spores were not found in smears prepared from the eggs laid by infected females, but cryosections demonstrated parasite cells within the host egg’s cytoplasm. A short region of N. pyrausta hexokinase was amplified using cDNA samples from infected females’ eggs, confirming the expression of the parasite’s DNA. Thus, vertical transmission of N. pyrausta is achieved through infection of insect eggs with prespore stages of the parasite.

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“…We used insects from a lab population at the All-Russian Institute of Plant Protection (St. Petersburg, Russia). Rearing of larvae was performed on an artificial diet in accordance with the procedure of Frolov et al (2019) 18 .…”

Section: Ostrinia Nubilalismentioning

confidence: 99%

A new microbiological weapon against lepidopteran pests

Martemyanov

Akhanaev

Belousova

et al. 2022

Preprint

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Nowadays researchers provide more and more evidence that it is necessary to develop an ecologically friendly approach to pest control. This is reflected in a sharp increase in the value of the biological insecticide market in recent decades. In our study, we found a virus strain belonging to the genus Cypovirus (Reoviridae); the strain was isolated from Dendrolimus sibiricus: that possesses attractive features as a candidate for mass production of biological agents for lepidopteran-pest control. We describe morphological, molecular, and ecological features of the new Cypovirus strain. This strain was found to be highly virulent to D. sibiricus (half-lethal dose is 68 occlusion bodies per second-instar larva) and to have a relatively wide host range (infects representatives of five families of Lepidoptera: Erebidae, Sphingidae, Pieridae, Noctuidae, and Lasiocampidae). The virus strain showed a strong interaction with a nontoxic adjuvant (optical brightener), which decreased the lethal dose for both main and alternative hosts, decreased lethal time, and may expand the host range. Moreover, we demonstrated that the insecticidal features were preserved after passaging through the most economically suitable host. By providing strong arguments for possible usefulness of this strain in pest control, we call on virologists, pest control specialists and molecular biologists to give more attention to the Cypovirus genus, which may lead to new insights in the field of pest control research and may provide significant advantages to compare with baculoviruses and Bacillus thuringiensis products which are nowadays main source of bioinsecticides.

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Rearing of Trilobed Male Uncus Ostrinia Species in Laboratory for Experimental Purposes

Cited by 8 publications

References 0 publications

Susceptibility of the Gypsy Moth Lymantria dispar (Lepidoptera: Erebidae) to Nosema pyrausta (Microsporidia: Nosematidae)

Susceptibility of the Gypsy Moth Lymantria dispar (Lepidoptera: Erebidae) to Nosema pyrausta (Microsporidia: Nosematidae)

Transovarial transmission of Nosema pyrausta in three generations of Ostrinia nubilalis in laboratory tests

A new microbiological weapon against lepidopteran pests

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