1990
DOI: 10.1111/j.1439-0418.1990.tb00023.x
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Rearing of Triatoma infestans Klug (Het., Reduviidae) in the absence of a live host

Abstract: The gorging response of 3rd‐instar nymphs of the vector of Chagas' disease Triatoma infestans Klug to different diets was analysed using an artificial feeder which allows one to feed a great number of bugs simultaneously. Citrated blood was more acceptable than heparinated blood, either stored at 4°C or at –20°C. Freezing of blood at –20°C and their subsequent haemolysis did not affect the intake of citrated blood when it was defrosted immediately before the experiment, but in fact when this occurred 36 hs bef… Show more

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Cited by 67 publications
(23 citation statements)
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“…Adults of Triatoma infestans of both sexes were reared in the laboratory at 28°C and fed on citrated sheep blood with the aid of an artificial feeder (Núñez & Lazzari 1990).…”
Section: Methodsmentioning
confidence: 99%
“…Adults of Triatoma infestans of both sexes were reared in the laboratory at 28°C and fed on citrated sheep blood with the aid of an artificial feeder (Núñez & Lazzari 1990).…”
Section: Methodsmentioning
confidence: 99%
“…Larvae of T. infestans, reared at 28°C and fed on heparinized bovine blood by means of an artificial feeder (Núñez & Lazzari 1990), were obtained from our laboratory colony.…”
Section: Methodsmentioning
confidence: 99%
“…Insects were fed weekly on bovine blood, containing 2% sodium citrate as an anticoagulant and supplemented with 100 mM adenosine 5′-tryphosphate disodium salt (Calbiochem, La Jolla, CA), using an artificial feeder. 27 Experiments with fifth-instar (last larval stage) and adult T. rubida were conducted with insects caught around Tucson, Arizona. 21 To avoid crosscontamination with T. cruzi , these insects were kept apart from laboratory-reared animals.…”
Section: Insectsmentioning
confidence: 99%