Rearing &lt;em&gt;Ixodes scapularis,&lt;/em&gt; the Black-legged Tick: Feeding Immature Stages on Mice

Nuss, Andrew B.; Mathew, Manoj G.; Gulia-Nuss, Monika

doi:10.3791/55286-v

Cited by 2 publications

(2 citation statements)

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“…Other studies have explicitly described difficulties related to manual tick feeding on animals (Nilsson and Lundqvist 1978, Nuss et al 2017). Several changes were made to the capsule design between the present work and the work described by Poché et al (2023a) to 1) accommodate 2x the number of mating pairs, and 2) accommodate the larger tick species A. americanum , and 3) improve the survivorship of fully engorged females.…”

Section: Discussionmentioning

confidence: 99%

Development of a low-dose fipronil deer feed: Bait-screening and range-finding to determine the optimal formulation to control blacklegged ticks (Ixodes scapularis) feeding on white-tailed deer (Odocoileus virginianus)

Poché,

Wagner,

Hawthorne

et al. 2023

Journal of Vector Ecology

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Lyme disease is the most prevalent vector-borne disease in the U.S., and acaricidal feeds administered to whitetailed deer (Odocoileus virginianus) have potential to disrupt blood feeding by the blacklegged tick, Ixodes scapularis. Two studies were conducted with the aim of determining an ideal formulation to deliver oral acaricides to white-tailed deer and finding the lowest fipronil dose level to effectively control I. scapularis. During formulation screening, various commercial attractants (baits) were presented to deer in large paddocks under semi-field conditions and motion-sensitive cameras were used to monitor consumption by deer and non-target species. During dose range-finding, deer were housed individually and presented a fipronil feed at one of five dose levels for 48 h (two deer untreated). At 24 h post-exposure, the deer were anesthetized, blood was drawn, and deer were manually infested with 20 I. scapularis mating pairs in feeding capsules. Tick mortality was monitored up to nine days post-attachment. Results of formulation screening indicated that loose/granular formulations were substantially more palatable to deer, relative to block formulations, and would be more effective under field conditions. Dose range-finding concluded fipronil feeds with doses of 25 ppm and higher would eliminate 100% of I. scapularis parasitizing deer. Additionally, 10 ppm fipronil feed controlled a considerable proportion of ticks, and results suggested 100% ticks could be eliminated if fipronil sulfone was present in plasma at ≥25.1 ppb. These results were paramount in developing a low dose fipronil deer feed for tick control and should provide valuable insights prior to execution of future field trials.

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Section: Discussionmentioning

confidence: 99%

Development of a low-dose fipronil deer feed: Bait-screening and range-finding to determine the optimal formulation to control blacklegged ticks (Ixodes scapularis) feeding on white-tailed deer (Odocoileus virginianus)

Poché,

Wagner,

Hawthorne

et al. 2023

Journal of Vector Ecology

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“…The first technique is an effective natural system for tick feeding but does not allow close monitoring during the experiment because the individual ticks are dispersed in different parts of the host body. Additionally, engorged ticks that drop to a recovery area can become contaminated with feces and urine 10 , 11 , 12 , 13 , 14 that may severely affect the tick fitness or they can be damaged or eaten by the mouse if there is no separation between the animal and the recovery area 15 .…”

Section: Introductionmentioning

confidence: 99%

A Capsule-Based Model for Immature Hard Tick Stages Infestation on Laboratory Mice

Mateos-Hernández

Rakotobé

Defaye

et al. 2020

JoVE

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Rearing <em>Ixodes scapularis,</em> the Black-legged Tick: Feeding Immature Stages on Mice

Cited by 2 publications

References 0 publications

Development of a low-dose fipronil deer feed: Bait-screening and range-finding to determine the optimal formulation to control blacklegged ticks (Ixodes scapularis) feeding on white-tailed deer (Odocoileus virginianus)

Development of a low-dose fipronil deer feed: Bait-screening and range-finding to determine the optimal formulation to control blacklegged ticks (Ixodes scapularis) feeding on white-tailed deer (Odocoileus virginianus)

A Capsule-Based Model for Immature Hard Tick Stages Infestation on Laboratory Mice

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