Rearing density influences the expression of stress-related genes in sea bass (Dicentrarchus labrax, L.)

Gornati, Rosalba; Papis, Elena; Rimoldi, Simona; Terova, Genciana; Saroglia, Marco; Bernardini, Giovanni

doi:10.1016/j.gene.2004.06.020

Cited by 157 publications

(86 citation statements)

References 21 publications

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“…Our group demonstrated by Real-Time PCR an increased expression of inducible HSP70 mRNA in 40 day larvae and 80 day fry of sea bass exposed to transport stress (Poltronieri et al 2007); moreover, muscle and skin of stressed adults showed an higher significant expression of inducible HSP70 mRNA respect than controls (Poltronieri et al, 2007). In the same species, Gornati et al (2004) detected an increased expression of HSP70 after overcrowding exposure.…”

mentioning

confidence: 78%

“…Podrabsky and Somero (2007) found that an inducible or embryo-specific form of HSP70 is expressed during embryonic development in killifish (Austrofundulus limnaeus) and is elevated during diapause II in this species. Gornati et al (2004Gornati et al ( , 2005 detected a different expression of HSP70 and HSP90 mRNAs in sea bass farmed at different stocking densities. Moreover, handling and stocking density procedures can affect cortisol levels as suggested by several authors (Marino et al 2001, Skjervold et al 2001, Simontacchi et al 2008.…”

mentioning

confidence: 98%

“…In our experimental condition the stress response to transport has been evaluated by measuring plasma cortisol levels demonstrating a significantly increased of cortisol levels in stressed animals (these results are object of another paper already submitted for publication). The expression of HSP70 mRNA has been studied in numerous fish species subjected to different stress conditions (Sanders et al 1995, Williams et al 1996, Vijayan et al 1997, Schmidt et al 1998, Duffy et al 1999, Hassanein et al 1999, Currie et al 2000, Ali et al 2003a, b, Zarate and Bradley 2003, Gornati et al 2004, Deane and Woo 2005, Ojima et al 2005). Our group demonstrated by Real-Time PCR an increased expression of inducible HSP70 mRNA in 40 day larvae and 80 day fry of sea bass exposed to transport stress (Poltronieri et al 2007); moreover, muscle and skin of stressed adults showed an higher significant expression of inducible HSP70 mRNA respect than controls (Poltronieri et al, 2007).…”

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confidence: 99%

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Immunohistochemical localization of constitutive and inducible Heat Shock Protein 70 in carp (Cyprinus carpio) and trout (Oncorhynchus mykiss) exposed to transport stress

Poltronieri¹,

Negrato²,

Bertotto³

et al. 2009

Eur J Histochem

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In the present work we investigated by immunohistochemistry the cellular localization of constitutive as well as inducible heat shock protein 70 in several tissues of common carp (Cyprinus carpio) and rainbow trout (Oncorhynchus mykiss) exposed to transport stress. In carp, the constitutive form (HSC70) was detected only in red skeletal muscle of both control and stressed animals. In the same species, the inducible form (HSP70) was evident in the epithelia of renal tubules, gills and skin of stressed animals, whereas in controls only red skeletal muscle exhibited an immunopositivity to HSP70 antibody. In trout, immunostaining to HSC70 antibody was found mainly in the epithelia of intestine, gills and skin of both control and stressed animals although the reactivity was generally higher in animals exposed to transport stress. In the same species immunostaining to HSP70 antibody was observed only in red skeletal muscle and epidermis of control animals.

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confidence: 78%

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confidence: 98%

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confidence: 99%

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Immunohistochemical localization of constitutive and inducible Heat Shock Protein 70 in carp (Cyprinus carpio) and trout (Oncorhynchus mykiss) exposed to transport stress

Poltronieri¹,

Negrato²,

Bertotto³

et al. 2009

Eur J Histochem

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“…Thus, HSP70 has been most widely used as a bioindicator of stress. HSP70 is induced by heat and chemical shocks in fish, like in mammals (Gornati et al, 2004). Gornati et al (2004) observed that HSP70 was also shown to be inducible by increasing stocking density in sea bass.…”

Section: Resultsmentioning

confidence: 99%

Fasted and postprandial response of serum physiological response, hepatic antioxidant abilities and HSP70 expression in Wuchang bream (Megalobrama amblycephala) fed different dietary carbohydrate levels

Zhou

Liu

et al. 2014

R. Bras. Zootec.

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-The effect of dietary carbohydrate (CHO) level on serum physiological response, hepatic antioxidant abilities and heat shock protein 70 (HSP70) expression of Wuchang bream (Megalobrama amblycephala) was studied. Two isonitrogenous (28.56% crude protein) and isolipidic (5.28% crude lipid) diets were formulated to contain 30% or 53% wheat starch. Diets were fed for 90 days to fish in triplicate tanks (28 fish per tank). At the end of feeding trial, significantly higher serum triglyceride level, insulin level, cortisol level, and malondialdehyde (MDA) content were observed in fish fed the 53% CHO diet, while significantly lower serum total protein content, alkaline phosphatase activity, superoxide dismutase activity and total antioxidative capacity were found in fish fed the 53% CHO diet compared with those fed the 30% diet. The relative level of hepatic heat shock protein 70 mRNA was significantly higher in the 53% CHO group than that in the 30% CHO at 6, 12 and 48 h after feeding. Ingestion of 53% dietary CHO impacts the nonspecific immune ability and causes metabolic stress in Megalobrama amblycephala.

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“…It has been reported that the constitutive HSPs are expressed under normal conditions and appear to be essential for protein folding or trafficking and regulated proteolysis in unstressed cells (Craig et al, 1983;Lindquist and Craig, 1988;Hightower, 1993). Constitutive and inducible isoforms of the HSP70 were also reported in non-mammalian organisms such as fish (Gornati et al, 2004;Yamashita et al, 2004), and molluscs (Clegg et al, 1998;Gourdon et al, 2000;Boutet et al, 2003).…”

Section: Discussionmentioning

confidence: 99%

Construction of a full-length cDNA library of Solen grandis Dunker and identification of defense- and immune-related genes

Sun

Liu

Ren

et al. 2013

J. Ocean Univ. China

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The basic genetic characteristics, important functional genes, and entire transcriptome of Solen grandis Dunker were investigated by constructing a full-length cDNA library with the 'switching mechanism at the 5'-end of the RNA transcript' (SMART) technique. Total RNA was isolated from the immune-relevant tissues, gills and hemocytes, using the Trizol reagent, and cDNA fragments were digested with Sfi I before being ligated to the pBluescript II SK* vector. The cDNA library had a titer of 1048 cfu μL −1 and a storage capacity of 1.05×10 6 cfu. Approximately 98% of the clones in the library were recombinants, and the fragment lengths of insert cDNA ranged from 0.8 kb to 3.0 kb. A total of 2038 expressed sequence tags were successfully sequenced and clustered into 965 unigenes. BLASTN analysis showed that 240 sequences were highly similar to the known genes (E-value < 1e −5 ; percent identity >80%), accounting for 25% of the total unigenes. According to the Gene Ontology, these unigenes were related to several biological processes, including cell structure, signal transport, protein synthesis, transcription, energy metabolism, and immunity. Fifteen of the identified sequences were related to defense and immunity. The full-length cDNA sequence of HSC70 was obtained. The cDNA library of S. grandis provided a useful resource for future researches of functional genomics related to stress tolerance, immunity, and other physiological activities.

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Rearing density influences the expression of stress-related genes in sea bass (Dicentrarchus labrax, L.)

Cited by 157 publications

References 21 publications

Immunohistochemical localization of constitutive and inducible Heat Shock Protein 70 in carp (Cyprinus carpio) and trout (Oncorhynchus mykiss) exposed to transport stress

Immunohistochemical localization of constitutive and inducible Heat Shock Protein 70 in carp (Cyprinus carpio) and trout (Oncorhynchus mykiss) exposed to transport stress

Fasted and postprandial response of serum physiological response, hepatic antioxidant abilities and HSP70 expression in Wuchang bream (Megalobrama amblycephala) fed different dietary carbohydrate levels

Construction of a full-length cDNA library of Solen grandis Dunker and identification of defense- and immune-related genes

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