Rearing and Colonization of Lutzomyia evansi (Diptera: Psychodidae), a Vector of Visceral Leishmaniasis in Colombia

Montoya-Lerma, James; Cadena-Peña, Horacio; Jaramillo-Salazar, Consuelo

doi:10.1590/s0074-02761998000200025

Cited by 9 publications

(10 citation statements)

References 12 publications

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“…The M:F ratio was higher for N. intermedia, although Rangel et al (1985) found that 45.1% of the emerging adults of this species in the laboratory were females. Males emerged before females, as has been reported by other authors (Chaniotis & Anderson 1964, Killick-Kendrick et al 1977, Montoya-Lerma et al 1998, Alves et al 2000.…”

Section: Discussionsupporting

confidence: 81%

Biology of the first generation of a laboratory colony of Nyssomyia intermedia (Lutz & Neiva, 1912) and Nyssomyia neivai (Pinto, 1926) (Diptera: Psychodidae)

Filho

Galati

Falcão

2004

Mem. Inst. Oswaldo Cruz

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Section: Discussionsupporting

confidence: 81%

Biology of the first generation of a laboratory colony of Nyssomyia intermedia (Lutz & Neiva, 1912) and Nyssomyia neivai (Pinto, 1926) (Diptera: Psychodidae)

Filho

Galati

Falcão

2004

Mem. Inst. Oswaldo Cruz

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“…However, due to the great difficulty of maintaining most sand fly colonies for longer than a few generations, life-table studies on sand flies are scarcest. For New World sand flies, the studies of KillickKendrick et al (1977) on L. longipalpis (Lutz & Neiva) and Cárdenas et al (1999) on L. shannoni (Dyar) have been focused on development times, reproductive rate, life tables and reproductive parameters; whereas the studies of Morales et al (1984), Neira et al (1998), Montoya et al (1998), Cabrera et al (1999, and Cabrera and Ferro (2000) have been focused on life cycle and development time of several species of Lutzomyia from Colombia. Data on life cycle and reproductive biology for phlebotomines are nearly impossible to obtain in the field because under these conditions, the immature stages have not been found.…”

Section: Discussionmentioning

confidence: 99%

“…KillickKendrick et al (1977) were successful for the first time on colonizing and reproducing L. longipalpis (Lutz & Neiva). Their work was the basis for the establishment in Colombia of colonies of other species of Lutzomyia, such as those achieved by the following authors: Morales et al (1984) with L. walkeri, Neira et al (1998) with L. torvida and L. longiflocosa, Montoya et al (1998) with L. evansi, Ferro et al (1998) and Cárdenas et al (1999) with L. shannoni, Cabrera et al (1999) with L. ovallesi and Cabrera and Ferro (2000) with three species of Lutzomyia of the group verrucarum.…”

mentioning

confidence: 99%

Life tables and reproductive parameters of Lutzomyia spinicrassa (Diptera: Psychodidae) under laboratory conditions

Escovar

Bello

Morales

et al. 2004

Mem. Inst. Oswaldo Cruz

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“…Secondly, our studies on the biology of Lu. evansi suggest that this sand fly has a very short life expectancy in nature (Oviedo et al 1995, Montoya-Lerma et al 1998 and finally, Lu. evansi could experimentally harbour and develop Leishmania mexicana but parasites did not reach its oesophagus (unpublished data).…”

mentioning

confidence: 99%

“…In addition, since the gonadotrophic cycle of Lu. evansi lasts on average only 3-4 days (Montoya-Lerma et al 1998), it is likely that the full development, migration, and colonization of the L. mexicana in the digestive tract of Lu. evansi requires a second bloodmeal.…”

mentioning

confidence: 99%

Effect of a second bloodmeal on the oesophagus colonization by Leishmania mexicana complex in Lutzomyia evansi (Diptera: Psychodidae)

Vivenes¹,

Oviedo²,

Márquez³

et al. 2001

Mem. Inst. Oswaldo Cruz

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Migration and colonization of the oesophagus byThe vectorial capacity of any leishmaniasis vector depends not only on its ability to develop and harbour parasites in its intestine but also the colonization of the foregut by infective promastigotes after a complete digestion of the infective bloodmeal. Also, an infected fly should be able to locate, bite and transmit the parasites to a new reservoir or host. Hence, both biochemical and physiological mechanisms might be expected to control host-seeking behaviour and parasite development in a vector, respectively. In the study of vector capacity two assumptions are widely accepted: firstly, at least for haematophagous Diptera, host seeking is stopped by a full bloodmeal (Klowden 1990) and, secondly, that a full infecting bloodmeal is sufficient for an appropriate sand fly vector to become infective. However, recent observations prompted us to revisit these assumptions in sand flies. Firstly, in two geographically separated areas in Colombia and Venezuela, hungry gravid and semi-gravid Lutzomyia evansi females were caught indoors hence, displaying an active host-seeking behaviour (Montoya-Lerma 1996, M Oviedo, pers. obs.). Secondly, our studies on the biology of Lu. evansi suggest that this sand fly has a very short life expectancy in nature (Oviedo et al. 1995, Montoya-Lerma et al. 1998 and finally, Lu. evansi could experimentally harbour and develop Leishmania mexicana but parasites did not reach its oesophagus (unpublished data). Since Lu. evansi has been reported coexisting in an area where L. mexicana occurs, all of these observations prompted us to investigate whether a second blood-meal taken by Lu. evansi, subsequent to an infective one, may influence the oesophageal colonization of L. mexicana and related parasites.For experimental and practical reasons, Syrian hamsters were infected either with one of two WHO L. mexicana reference strains (i.e. L. amazonensis IFLA/BR/67/PHB and L. mexicana MHOM/VE/ 72/AZV) and used as source of parasites. Hamsters were anaesthetised and its posterior footpad introduced, for approximately 20 min, inside a glass devise, containing starved bred female Lu. evansi. Fed flies were maintained in darkness, under constant conditions (26ºC and 85% RH). Putatively infected females were fed with sucrose as food source and dissected, to evaluate parasite development in their gut, at 24 h intervals from 72 h until 168 h post-infection. Afterwards, surviving females were fed on an uninfected hamster. Refed flies were kept as indicated above, receiving sucrose solution. After 72 h and during seven days, dissections were continued, following the same 24 h schedule.Results showed that susceptibility of Lu. evansi to infection ranged between 29.4% (n= 119) and 21.7% (n= 147) to L. mexicana and L. amazonensis, respectively. The typical suprapylarian development was exhibited though presence of free flagel-

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Rearing and Colonization of Lutzomyia evansi (Diptera: Psychodidae), a Vector of Visceral Leishmaniasis in Colombia

Cited by 9 publications

References 12 publications

Biology of the first generation of a laboratory colony of Nyssomyia intermedia (Lutz & Neiva, 1912) and Nyssomyia neivai (Pinto, 1926) (Diptera: Psychodidae)

Biology of the first generation of a laboratory colony of Nyssomyia intermedia (Lutz & Neiva, 1912) and Nyssomyia neivai (Pinto, 1926) (Diptera: Psychodidae)

Life tables and reproductive parameters of Lutzomyia spinicrassa (Diptera: Psychodidae) under laboratory conditions

Effect of a second bloodmeal on the oesophagus colonization by Leishmania mexicana complex in Lutzomyia evansi (Diptera: Psychodidae)

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