Reappraisal of the Biological
Role of Epidermal Growth
Factor Receptor in Transitional
Cell Carcinoma

Chow, Nan‐Haw; Tzai, Tzong-Shin; Lin, Shinn-Nan; Chan, Siu-Hong; Tang, Min

doi:10.1159/000474281

Cited by 15 publications

(10 citation statements)

References 16 publications

(22 reference statements)

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“…The staging of tumors was determined by simultaneous review of the clinical details, image studies, and pathological data, and judged according to the TNM protocol from the American Joint Committee on Cancer (1983). The procedure for extracting tumor nuclei for DNA flow cytometry and the interpretation criteria have been described pre viously [18]. The DNA histogram was studied using FACScan (Becton-Dickinson Immunocytochemistry System, Mountain View, Cal if.)…”

Section: Urine Cytologymentioning

confidence: 99%

“…The alkaline phosphatase anti-alkaline phosphatase method was utilized for labelling. Interpretation of the staining result was based on criteria described in a previous report [18]. Briefly, positive The numerator represents cases of positive urinary cytology, and denominator as total study cases.…”

Section: Immunohistochemistrymentioning

confidence: 99%

“…Mea surement can offer additional, objective data concerning the malignant potential of tumors. Expression of the receptor for epidermal growth factor (EGFR) on tumor cells could contribute to tumor proliferation [18] and invasive potential [19][20][21], and is significantly related to an unfavorable outcome [22], This study was undertaken to examine the correlation of urinary cytology with a small number of established factors and the above biological markers of bladder can cer. The prognostic importance or urinary cytology would then be evaluated in relation to patients with bladder tumor or a subset of Ta-Tl tumors.…”

Section: Introductionmentioning

confidence: 99%

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Urinary Cytodiagnosis: Can It Have a Different Prognostic Implication than a Diagnostic Test?

Chow¹,

Tzai²,

Cheng³

et al. 1994

Urol Int

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In order to assess the clinical implications of the cytology of voided urine, we analyzed 65 patients among 147 cases of transitional cell carcinoma (TCC) in relation to the cytohistologic correlation and prognostic significance. Urinary cytology detected 42.6% of bladder tumors, 59% of renal pelvic tumors, and 35.3% of ureteral carcinomas. None of the 3 cases of grade-1 upper urinary tract tumors was detected by preoperative urinary cytology. Tumors with positive cytology were associated with epidermal growth factor receptor expression (p = 0.0009) and a higher fraction of tumor proliferation as defined by Ki-67 immunohistochemistry (p = 0.0038). Anaplastic tumor cells in urine correlated fairly well with muscular invasion (p = 0.018) and the DNA aneu-ploidy of the tumor (p = 0.0038). Tumors with muscular invasion were more likely to be detected by cytologic examination (p = 0.013). In urinary bladder carcinoma (n = 107), patients with positive cytology had a higher incidence of tumor recurrence (p = 0.004), and had an unfavorable prognosis (p = 0.0001) with a median follow-up of 46 months. In Ta-T1 tumors (n = 87), urinary cytology had prognostic value in terms of risk of recurrence (p = 0.0001), and poor patient outcome (p = 0.0001). Our data suggest that urinary cytology can offer important biological information on TCC and may be used as a simple and effective short-term prognostic indicator.

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Section: Urine Cytologymentioning

confidence: 99%

Section: Immunohistochemistrymentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Urinary Cytodiagnosis: Can It Have a Different Prognostic Implication than a Diagnostic Test?

Chow¹,

Tzai²,

Cheng³

et al. 1994

Urol Int

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“…The level of EGFR correlates with stage, grade, and progression of human transitional cell carcinoma (11,12). Dinney et al (13) reported previously that therapy with either protein tyrosine kinase inhibitors or anti-EGFR monoclonal antibodies inhibits the growth of established human transitional cell carcinoma growing orthotopically in athymic nude mice (14).…”

Section: Introductionmentioning

confidence: 99%

Effect of an epidermal growth factor receptor tyrosine kinase inhibitor on actin remodeling in anin vitrobladder cancer carcinogenesis model

Jin¹,

Iwata

Belldegrun

et al. 2006

Molecular Cancer Therapeutics

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Alteration of actin remodeling is a marker of malignantassociated field defect and a potential surrogate biomarker for chemoprevention trials. We tested erlotinib, a specific tyrosine kinase inhibitor of epidermal growth factor receptor (EGFR), on actin remodeling in a bladder carcinogenic model consisting of untransformed HUC-PC cells and transformed MC-T11 cells, both derived from the same normal human urothelial clone immortalized by SV40. Erlotinib had a selective growth inhibitory and actin remodeling effect on MC-T11 cells over HUC-PC cells, as examined by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay and immunofluorescence labeling with laser scan cytometer analysis, respectively. The IC 50 of untransformed HUC-PC cells was significantly higher than that of transformed MC-T11 cells (P < 0.05, t test). The actin remodeling effect was more prominent at lower dosage levels (1/8-1/4 of IC 50 ), which was accompanied by an increased cell adhesion and decreased motility. At higher dosage levels (1/2 of IC 50 ), erlotinib induced a decreased adhesion and anoikis (detachmentassociated apoptosis). The transformed MC-T11, but not HUC-PC, showed a weak constitutive EGFR phosphorylation activity, which was inhibited by erlotinib in a doseresponse manner. However, on epidermal growth factor stimulation, both cell lines showed a similar dose-response inhibitory effect on phosphorylated EGFR and mitogenactivated protein kinase (MAPK; P44/P42) activities, and MAPK inhibitor PD98059 showed no specific effect on erlotinib-induced actin remodeling, suggesting that pathways other than MAPK (P44/P42) may be responsible for erlotinib-induced actin remodeling. The findings provide evidence to support erlotinib-based bladder cancer chemoprevention and using actin remodeling as a marker for erlotinib-based intervention trials.

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“…Homozygous deletion of EGFR is lethal, and knockout mice die as embryos or shortly after birth due to severe epithelial developmental defects (29 -31). Paradoxically, aberrant EGFR signaling has been correlated with tumor progression to invasion and metastasis (32)(33)(34)(35), and EGFR is overexpressed in a wide variety of tumors (36 -39).…”

mentioning

confidence: 99%

Epidermal Growth Factor Receptor Activation Differentially Regulates Claudin Expression and Enhances Transepithelial Resistance in Madin-Darby Canine Kidney Cells

Singh

Harris

2004

Journal of Biological Chemistry

174

150

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Reappraisal of the Biological Role of Epidermal Growth Factor Receptor in Transitional Cell Carcinoma

Cited by 15 publications

References 16 publications

Urinary Cytodiagnosis: Can It Have a Different Prognostic Implication than a Diagnostic Test?

Urinary Cytodiagnosis: Can It Have a Different Prognostic Implication than a Diagnostic Test?

Effect of an epidermal growth factor receptor tyrosine kinase inhibitor on actin remodeling in anin vitrobladder cancer carcinogenesis model

Epidermal Growth Factor Receptor Activation Differentially Regulates Claudin Expression and Enhances Transepithelial Resistance in Madin-Darby Canine Kidney Cells

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