Realization of noncontact confocal optical microsphere imaging microscope

Abstract

A novel noncontact confocal optical microsphere imaging microscope (COMIM) is proposed to achieve high contrast optical nano‐imaging at a high scanning speed. The developed setup obtains images with significantly higher contrast when compared with conventional bright‐field microsphere nano‐imaging, as well as with a commercial confocal laser scanning microscope (CLSM). With the image‐by‐image scanning scheme, COMIM takes only 11% time to complete a three‐dimensional (3D) scanning image than using a commercial CLSM, while still offers an outstanding resolving power for the nano‐features on the sample.

Optical scanning endoscope via a single multimode optical fiber

“…Moreover, the thermal drift issue often occurs in the SLM because it needs to frequently update masks during scanning imaging. This problem can be solved by using a thermoelectric cooler to stabilize the temperature of the SLM 124 . Finally, fluctuations in environmental refractive index and temperature may also affect the imaging performance of MFSEs.…”

Advances in the application of manganese dioxide and its composites for theranostics

“…17 Molecular imaging is a valuable method in disease diagnosis to assess metabolic changes and detect disease progression. With rapid advancements in nanotechnology, MITs including photoacoustic imaging (PA), 18 fluorescence (FL) imaging, 19 single-photon emission computed tomography, 20 optical microscopy, 21 positron emission computed tomography 22 and magnetic resonance imaging (MRI), 23 have attracted significant attention based on nanometer-scale contrast agents. Among them, MRI contrast agents can provide anatomical structures and pathology with a high spatial resolution, [24][25][26] these agents are used in the fields of inflammation detection, 27 fibrosis diagnosis, 28 thromboembolic disease imaging 29 and cancer therapy.…”

光学显微图像定量评价方法及应用研究进展（特邀）

“…9 Two kinds of contrast evaluation methods. (a) Weber contrast values of different samples [48] ; (b) images of fluorescent beads covered with fluorescein in solution outside (left) and inside (right) the structure [49] ; (c) Michelson contrast curve and image display of bright field technology and confocal imaging [50] 0618013 -9…”