Realization of Low-Cost Multichannel Surface Plasmon Resonance Based Optical Transducer

Somarapalli, Manjunath; Jolivot, Romuald; Mohammed, Waleed S.

doi:10.1007/s13320-018-0511-z

Cited by 4 publications

(4 citation statements)

References 27 publications

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“…Thus, the RI resolution of the two instruments was broadly comparable. The RI resolution of the LW obtained using the 3D printed instrument was comparable to the values reported in literature for 3D printed SPR instrument [30][31][32][33][34][35][36] (2.37×10 -6 RIU versus 1.7×10 −6 RIU). Based on the RI resolution of the LW obtained using the 3D printed instrument and typical (dn/dc) of 1.89×10 -4 L g −1 for proteins [40], we estimate the LOD for IgG (M w : 150,000 g) to be ~83 nM in the absence of antibodies to enhance the local concentration of protein in the waveguide.…”

Section: Lw Optimization and Characterizationsupporting

confidence: 85%

“…As a result, 3D printing has increasingly been used to develop a wide range of biochemical analytical systems [27][28][29]. For example, 3D printing has been used to develop point-of-use instrumentation for SPR with a resolution of 6.4×10 −6 to 10 −4 RIU −1 [30,31]. The low cost of SLA and FFF 3D printers means that instruments can be produced in low resource settings without requiring dedicated production lines, and that designs can be modified quickly as requirements change.…”

Section: Introductionmentioning

confidence: 99%

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3-D Printed Instrumentation for Point-of-Use Leaky Waveguide Biochemical Sensor

Goddard

Dixon

Toole

2020

IEEE Trans. Instrum. Meas.

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Leaky waveguide (LW) biosensors enable accurate measurements using small sample volumes and are cheap to produce, hence are advantageous in the area of point-of-use devices. Yet, current instrumentation to test LW chips is both bulky and costly, because of the use of expensive components such as glass optics and manufacturing techniques such as computer numerical control (CNC) machining. Meanwhile, 3D printing allows the production of complex shapes that cannot be realised using these techniques, while injection moulding allows the low cost production of optical components. 3D printed instruments offer huge advantages over traditional laboratory instrumentation, in terms of the cost of the manufacturing equipment required, the cost of the resulting instrumentation, size, and portability. This study describes the design and manufacture of a novel 3D printed biosensor instrument, and demonstrates its use for bioanalysis using LWs with a chitosan waveguide layer. This instrument has a refractive index resolution comparable to laboratory instrumentation and 3D printed surface plasmon resonance (SPR) instruments (2.37×10 −6 , 5.90×10 −6 and 1.7×10 −6 refractive index units [RIU] respectively) and has proven able to detect 133 nM (nmol L −1 ) levels of Immunoglobulin G (IgG) via the measurement of the change in resonance angle produced upon the protein binding to the film.

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Section: Lw Optimization and Characterizationsupporting

confidence: 85%

Section: Introductionmentioning

confidence: 99%

3-D Printed Instrumentation for Point-of-Use Leaky Waveguide Biochemical Sensor

Goddard

Dixon

Toole

2020

IEEE Trans. Instrum. Meas.

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“…Manjunath developed low-cost spectrometers using diffraction gratings and webcams. He built a dual-channel SPR system with a sensitivity of 10 −4 RIU using two plastic fibers [28]. Zhang et al used 3D printing technology of molten bulk molding to make some mechanical parts required by the system to reduce the cost and shorten the development cycle.…”

Section: Introductionmentioning

confidence: 99%

Development of a Portable SPR Sensor for Nucleic Acid Detection

Huang

Zhang

et al. 2020

Micromachines

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Nucleic acid detection is of great significance in clinical diagnosis, environmental monitoring and food safety. Compared with the traditional nucleic acid amplification detection method, surface plasmon resonance (SPR) sensing technology has the advantages of being label-free, having simple operation, and providing real-time detection. However, the angle scanning system in many SPR angle modulation detection applications usually requires a high-resolution stepper motor and complex mechanical structure to adjust the angle. In this paper, a portable multi-angle scanning SPR sensor was designed. The sensor only uses one stepping motor to rotate a belt, and the belt pulls the mechanical linkages of incident light and reflected light to move in opposite directions for achieving the SPR angle scanning mode that keeps the incident angle and reflected angle equal. The sensor has an angle scanning accuracy of 0.002°, response sensitivity of 3.72 × 10−6 RIU (refractive index unit), and an angle scanning range of 30°–74°. The overall size of the system is only 480 mm × 150 mm × 180 mm. The portable SPR sensor was used to detect nucleic acid hybridization on a gold film chip modified with bovine serum albumin (BSA). The result revealed that the sensor had high sensitivity and fast response, and could successfully accomplish the hybridization detection of target DNA solution of 0.01 μmol/mL.

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“…Somarapalli et al developed a multichannel SPR system consisting of a diffraction grating and a webcamera as a spectrophotometer. 7 Spectral images were recorded with the webcamera and analyzed with ImageJ software installed on a personal computer. This system allows one to measure any refractive index change on the order of 10 -4 .…”

mentioning

confidence: 99%