Realistic concentrations of Bisphenol-A trigger a neurotoxic response in the brain of zebrafish: Oxidative stress, behavioral impairment, acetylcholinesterase inhibition, and gene expression disruption

Heredia-García, Gerardo; Elizalde-Velázquez, Gustavo Axel; Gómez-Oliván, Leobardo Manuel; Islas-Flores, Hariz; García-Medina, Sandra; Galár-Martínez, Marcela; Dublán‐García, Octavio

doi:10.1016/j.chemosphere.2023.138729

Cited by 8 publications

(3 citation statements)

References 47 publications

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“…In studies with fish, authors such as Heredia-García et al [103] evaluated the neurotoxic effects of acute exposure to BPA (96 h) at environmentally relevant concentrations (220, 1180, and 1500 ng/L) in adult zebrafish (Danio rerio), subsequently performing swimming behavior assessment tests (novel tank). Their results indicated that exposure to 1500 ng/L of BPA reduces the total distance traveled and increases the stopping time of the fish, concluding that environmentally relevant BPA concentrations could cause anxiety-like behavior and neurotoxic effects in adult zebrafish.…”

Section: Developmental and Neurobehavioral Effectsmentioning

confidence: 99%

An Overview of the Health Effects of Bisphenol A from a One Health Perspective

Molina-López

Bujalance-Reyes

Ayala-Soldado

et al. 2023

Animals

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Bisphenol A (BPA) is a chemical compound, considered as an “emerging pollutant”, that appears ubiquitously, contaminating the environment and food. It is an endocrine disruptor, found in a multitude of consumer products, as it is a constituent of polycarbonate used in the manufacture of plastics and epoxy resins. Many studies have evaluated the effects of BPA, using a wide range of doses and animal models. In this work, we carried out a review of relevant research related to the effects of BPA on health, through studies performed at different doses, in different animal models, and in human monitoring studies. Numerous effects of BPA on health have been described; in different animal species, it has been reported that it interferes with fertility in both females and males and causes alterations in their offspring, as well as being associated with an increase in hormone-dependent pathologies. Similarly, exposure to BPA has been related to other diseases of great relevance in public health such as obesity, hypertension, diabetes, or neurodevelopmental disorders. Its ubiquity and nonmonotonic behavior, triggering effects at exposure levels considered “safe”, make it especially relevant when both animal and human populations are constantly and inadvertently exposed to this compound. Its effects at low exposure levels make it essential to establish safe exposure levels, and research into the effects of BPA must continue and be focused from a “One Health” perspective to take into account all the factors that could intervene in the development of a disease in any exposed organism.

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Section: Developmental and Neurobehavioral Effectsmentioning

confidence: 99%

An Overview of the Health Effects of Bisphenol A from a One Health Perspective

Molina-López

Bujalance-Reyes

Ayala-Soldado

et al. 2023

Animals

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“…Gene expression analysis related to axons has demonstrated alteration in the expression of regeneration-associated genes such as growth-associated protein Gap-43 ( gap43 ) ( Chen et al, 2012 ; Sun et al, 2016 ; Soares et al, 2017 ; Faria et al, 2018 ; Qian et al, 2018 ; Shi et al, 2018 ; Jin et al, 2019b ; Li et al, 2019 ; Cheng et al, 2020 ; Zhu et al, 2020 ; Chen et al, 2021 ; Gyimah et al, 2021 ; Li et al, 2021 ; Tian et al, 2021 ; Xu et al, 2021 ; Xu et al, 2022a ; Xu et al, 2022b ; Chang et al, 2022 ; Yu et al, 2022 ; Shi et al, 2023 ; Xiong et al, 2023 ; Xu et al, 2023 ; Zhu et al, 2023 ) and α1 tubulin ( Sun et al, 2016 ; Li et al, 2018 ; Shi et al, 2018 ; Yang et al, 2019 ; Fu et al, 2020 ; Zhu et al, 2020 ; Chen et al, 2021 ; Gyimah et al, 2021 ; Xu et al, 2021 ; Xu et al, 2022a ; Xu et al, 2022b ; Heredia-García et al, 2023 ; Shi et al, 2023 ; Xu et al, 2023 ). Additionally, nerve related genes like pax6a ( Ho et al, 2013 ; Li et al, 2019 ; Wang et al, 2021 ; Gyimah et al, 2022 ), elavl3 ( Chen et al, 2012 ; Sun et al, 2016 ; Qian et al, 2018 ; Shi et al, 2018 ; Yang et al, 2019 ; Zhu et al, 2020 ; Chen et al, 2021 ; Gyimah et al, 2021 ; Wang et al, 2021 ; Xu et al, 2021 ; Xu et al, 2022a ; Xu et al, 2022b ; Fan et al, 2022 ; Wang et al, 2022 ; Yu et al, 2022 ;…”

Section: Morphology and Characteristics Of Neuronsmentioning

confidence: 99%

“…The impact on the dopaminergic system can be investigated by analysing the morphology of dopaminergic neurons ( Cui et al, 2013 ; Yao et al, 2017 ; Jin et al, 2019a ; Wang et al, 2019 ), aided by the availability of zebrafish transgenic lines with GFP-labelled dopaminergic neurons. Analysis of dopamine levels ( Wang et al, 2015 ; Sheng et al, 2016 ; Soares et al, 2017 ; Wang et al, 2019 ; Han et al, 2021 ; Yang et al, 2022 ) and its metabolite, hydroxy-phenyl acetic acid, along with the expression of dopamine transporter ( dat ) genes ( Ji et al, 2021 ; Gyimah et al, 2022 ), dopamine receptors ( drd1b ( Li et al, 2019 ; Tran et al, 2021 ; Hong et al, 2022 ; Liu et al, 2023 ), drd2a ( Cao et al, 2019 ; Gyimah et al, 2022 ; Shi et al, 2022 ; Liu et al, 2023 ; Ding et al, 2023 ; Mahapatra et al, 2023 ; Torres-Ruiz et al, 2023 ; Xiang et al, 2023 ), drd2b [ Shi et al, 2022 ; Liu et al, 2023 ; Torres-Ruiz et al, 2023 )] and developmental genes correlated to dopamine such as manf ( Li et al, 2018 ; Qian et al, 2018 ; Li et al, 2020 ; Zhu et al, 2020 ; Tian et al, 2021 ; Hong et al, 2022 ; Heredia-García et al, 2023 ; Shi et al, 2023 ), otpa , otpb ( Wang et al, 2019 ), wnt1 ( Ho et al, 2013 ), wnt5b ( Ho et al, 2013 ), wnt10b ( Xu et al, 2022a ), wnt4a ( Xu et al, 2022a ), wnt3a , wnt5a ( Wang et al, 2019 ) can be analysed ( Supplementary Table S2 ). Treatment with lead in zebrafish larvae significantly reduced the number of dopaminergic neurons in the midbrain ( Jin et al, 2019b ).…”

Section: Neurotransmissionmentioning

confidence: 99%

Temperature- and chemical-induced neurotoxicity in zebrafish

Toni,

Arena,

Cioni

et al. 2023

Front. Physiol.

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Throughout their lives, humans encounter a plethora of substances capable of inducing neurotoxic effects, including drugs, heavy metals and pesticides. Neurotoxicity manifests when exposure to these chemicals disrupts the normal functioning of the nervous system, and some neurotoxic agents have been linked to neurodegenerative pathologies such as Parkinson’s and Alzheimer’s disease. The growing concern surrounding the neurotoxic impacts of both naturally occurring and man-made toxic substances necessitates the identification of animal models for rapid testing across a wide spectrum of substances and concentrations, and the utilization of tools capable of detecting nervous system alterations spanning from the molecular level up to the behavioural one. Zebrafish (Danio rerio) is gaining prominence in the field of neuroscience due to its versatility. The possibility of analysing all developmental stages (embryo, larva and adult), applying the most common “omics” approaches (transcriptomics, proteomics, lipidomics, etc.) and conducting a wide range of behavioural tests makes zebrafish an excellent model for neurotoxicity studies. This review delves into the main experimental approaches adopted and the main markers analysed in neurotoxicity studies in zebrafish, showing that neurotoxic phenomena can be triggered not only by exposure to chemical substances but also by fluctuations in temperature. The findings presented here serve as a valuable resource for the study of neurotoxicity in zebrafish and define new scenarios in ecotoxicology suggesting that alterations in temperature can synergistically compound the neurotoxic effects of chemical substances, intensifying their detrimental impact on fish populations.

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Chronic exposure to bisphenol A induces behavioural, neurochemical, histological, and ultrastructural alterations in the ganglia tissue of the date mussels Lithophaga lithophaga

Abd Elkader,

Al-Shami

2023

Environ Sci Pollut Res

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Bisphenol A (BPA), a common plastic additive, has been demonstrated mechanistically to be a potential endocrine disruptor and to affect a variety of body functions in organisms. Although previous research has shown that BPA is toxic to aquatic organisms, the mechanism of neurotoxic effects in marine bivalves remains unknown. The current study aimed to elucidate the neurotoxic effects of BPA when administered at different concentrations (0.25, 1, 2, and 5 µg/L) for twenty-eight days in the ganglia of a bivalve model, the Mediterranean mussel (Lithophaga lithophaga), which is an ecologically and economically important human food source of bivalve species in the Mediterranean Sea. Our findings revealed an increase in behavioural disturbances and malondialdehyde levels in treated mussel ganglia compared to the control group. Furthermore, superoxide dismutase activity increased in the ganglia of L. lithophaga treated with 0.25 and 2 µg/L. However, at BPA concentrations of 1 and 5 µg/L, SOD activity was significantly reduced, as was total glutathione concentration. BPA causes neurotoxicity, as evidenced by concentration-dependent inhibition of acetylcholinesterase, dopamine, and serotonin. After chronic exposure to BPA, neurons showed distortion of the neuronal cell body and varying degrees of pyknosis. The ultrastructure changes in BPA-treated groups revealed the lightening of the nucleoplasm and a shrunken nuclear envelope. Overall, our findings suggest that BPA exposure altered antioxidation, neurochemical biomarkers, histopathological, and ultrastructural properties, resulting in behavioural changes. As a result, our findings provide a basis for further study into the toxicity of BPA in marine bivalves.

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Realistic concentrations of Bisphenol-A trigger a neurotoxic response in the brain of zebrafish: Oxidative stress, behavioral impairment, acetylcholinesterase inhibition, and gene expression disruption

Cited by 8 publications

References 47 publications

An Overview of the Health Effects of Bisphenol A from a One Health Perspective

An Overview of the Health Effects of Bisphenol A from a One Health Perspective

Temperature- and chemical-induced neurotoxicity in zebrafish

Chronic exposure to bisphenol A induces behavioural, neurochemical, histological, and ultrastructural alterations in the ganglia tissue of the date mussels Lithophaga lithophaga

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