Real-time, smartphone-based processing of lateral flow assays for early failure detection and rapid testing workflows

Colombo, Monika; Bezinge, Léonard; Tapia, Andres Rocha; Shih, Chih‐Jen; Mello, Andrew J. de; Richards, Daniel A.

doi:10.1039/d2sd00197g

Cited by 10 publications

(5 citation statements)

References 25 publications

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“…Lateral flow profiles were measured using a 3D‐printed set‐up reported elsewhere, [ 69 ] and imaged using a smartphone camera (Samsung Galaxy A3 2017) at 30 frames per second. The strips were laser cut to a size of 4 × 40 mm and mounted on an adhesive backing card (KN‐2211, Kenosha) with a sample pad and absorbent pad (CF6, Cytiva).…”

Section: Methodsmentioning

confidence: 99%

Paper‐Based Laser‐Pyrolyzed Electrofluidics: An Electrochemical Platform for Capillary‐Driven Diagnostic Bioassays

et al. 2023

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Microfluidic paper-based analytical devices (μPADs) are indispensable tools for disease diagnostics. The integration of electronic components into μPADs enables new device functionalities and facilitates the development of complex quantitative assays. Unfortunately, current electrode fabrication methods often hinder capillary flow, considerably restricting μPAD design architectures. Here, laser-induced graphenization is presented as an approach to fabricate porous electrodes embedded into cellulose paper. The resulting electrodes not only have high conductivity and electrochemical activity, but also retain wetting properties for capillary transport. Paper-based electrofluidics, including a lateral flow device for injection analysis of alkaline phosphatase in serum and a vertical flow device for quantitative detection of HPV16 with a CRISPR-based assay are demonstrated. It is expected that this platform will streamline the development of diagnostic devices that combine the operational simplicity of colorimetric lateral flow tests with the added benefits and possibilities offered by electronic signaling.

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Section: Methodsmentioning

confidence: 99%

Paper‐Based Laser‐Pyrolyzed Electrofluidics: An Electrochemical Platform for Capillary‐Driven Diagnostic Bioassays

et al. 2023

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“…Smartphones have recently played an important role in portable LFA devices to obtain, evaluate, and exhibit measuring signals with high sensitivity [ 83 , 85 , 86 , 87 ]. For example, an automated computational imaging system relying on the continuous monitoring of test region intensity, background, control region attendance, and sample flow rates for real-time and, in parallel, evaluating multiple LFAs was introduced by Colombo et al [ 88 ]. The system ran on a smartphone by integrating simple 3D-printed housing and the custom code.…”

Section: Assay Improvementmentioning

confidence: 99%

Lateral Flow Assay: A Summary of Recent Progress for Improving Assay Performance

Omidfar,

Riahi,

Kashanian

2023

Biosensors

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Lateral flow tests are one of the most important types of paper-based point-of-care (POCT) diagnostic tools. It shows great potential as an implement for improving the rapid screening and management of infections in global pandemics or other potential health disorders by using minimally expert staff in locations where no sophisticated laboratory services are accessible. They can detect different types of biomarkers in various biological samples and provide the results in a little time at a low price. An important challenge regarding conventional LFAs is increasing their sensitivity and specificity. There are two main approaches to increase sensitivity and specificity, including assay improvement and target enrichment. Assay improvement comprises the assay optimization and signal amplification techniques. In this study, a summarize of various sensitivity and specificity enhancement strategies with an objective evaluation are presented, such as detection element immobilization, capillary flow rate adjusting, label evolution, sample extraction and enrichment, etc. and also the key findings in improving the LFA performance and solving their limitations are discussed along with numerous examples.

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“…Various Image processing algorithms for the analysis of lateral flow assay images have been developed and reported in the literature [11][12][13][14][15][16][17][18][19][20][21] ,most of which pertain to the development of smartphone applications for the accurate interpretation of the test results by the end-user. However, while the LFAs are being designed in the laboratory, the researchers need to 'run the standards,' i.e., perform the assay at several different analyte concentrations, in many replicates for each concentration to generate a standard calibration (signal-response) curve for the proposed assay.…”

Section: Introductionmentioning

confidence: 99%

Streamlining Lateral Flow Assay (LFA) Data Analysis: A MATLAB® Live Script for Integrated Batch Image Processing and Limit of Detection Determination

Saxena,

Toley

2024

Preprint

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Lateral flow assays (LFAs) are widely utilized for rapid point-of-care diagnostics in various fields; however, their quantitative image analysis is frequently hindered by repetitive manual processes and inconsistencies, typically involving the use of combinations of image processing and data analysis software tools. This protocol introduces a MATLAB live-script-based code for the integrated batch processing of LFA images and standard curve development. This code simplifies quantitative LFA analysis by automating image processing, peak detection, statistical analysis, and data fitting to a 4-parameter logistic curve for accurate Limit of Detection (LoD) determination. This approach significantly minimizes manual intervention and the potential for human error, thereby enhancing reproducibility. This addresses the critical need for efficient and reliable LFA data analysis in research laboratories. By offering a standardized method for LFA image processing and data analysis, this protocol facilitates faster and more consistent research outcomes.

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Real-time, smartphone-based processing of lateral flow assays for early failure detection and rapid testing workflows

Abstract: Despite their simplicity, lateral flow immunoassays (LFIAs) remain a crucial weapon in the diagnostic arsenal, particularly at the point-of-need. However, methods for analysing LFIAs still rely heavily on sub-optimal human...

Cited by 10 publications

References 25 publications

Paper‐Based Laser‐Pyrolyzed Electrofluidics: An Electrochemical Platform for Capillary‐Driven Diagnostic Bioassays

Paper‐Based Laser‐Pyrolyzed Electrofluidics: An Electrochemical Platform for Capillary‐Driven Diagnostic Bioassays

Lateral Flow Assay: A Summary of Recent Progress for Improving Assay Performance

Streamlining Lateral Flow Assay (LFA) Data Analysis: A MATLAB® Live Script for Integrated Batch Image Processing and Limit of Detection Determination

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