Real-time sensors for live monitoring of disease and drug analysis in microfluidic model of proximal tubule

Asif, Arun; Kim, Kyung Hwan; Jabbar, Faiza; Kim, Sejoong; Choi, Kyung Hyun

doi:10.1007/s10404-020-02347-1

Cited by 45 publications

(42 citation statements)

References 44 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The level of relevance of a microphysiological system (MPS) with human physiology makes them ideal for identifying the novel therapeutics. The organ-on-a-chip and spheroid culture systems are evolving to better mimic human pathophysiology, which is being enhanced by the integration of sensors and robotics [1][2][3][4][5][6][7][8]. Among MPS, spheroids are emerging with the potential to mimic human tissues in a 3D shape and function [9][10][11].…”

Section: Introductionmentioning

confidence: 99%

Gravity-Based Flow Efficient Perfusion Culture System for Spheroids Mimicking Liver Inflammation

Kim,

Asif,

Chethikkattuveli Salih

et al. 2021

Biomedicines

Self Cite

View full text Add to dashboard Cite

The spheroid culture system provides an efficient method to emulate organ-specific pathophysiology, overcoming the traditional two-dimensional (2D) cell culture limitations. The intervention of microfluidics in the spheroid culture platform has the potential to enhance the capacity of in vitro microphysiological tissues for disease modeling. Conventionally, spheroid culture is carried out in static conditions, making the media nutrient-deficient around the spheroid periphery. The current approach tries to enhance the capacity of the spheroid culture platform by integrating the perfusion channel for dynamic culture conditions. A pro-inflammatory hepatic model was emulated using a coculture of HepG2 cell line, fibroblasts, and endothelial cells for validating the spheroid culture plate with a perfusable channel across the spheroid well. Enhanced proliferation and metabolic capacity of the microphysiological model were observed and further validated by metabolic assays. A comparative analysis of static and dynamic conditions validated the advantage of spheroid culture with dynamic media flow. Hepatic spheroids were found to have improved proliferation in dynamic flow conditions as compared to the static culture platform. The perfusable culture system for spheroids is more physiologically relevant as compared to the static spheroid culture system for disease and drug analysis.

show abstract

Section: Introductionmentioning

confidence: 99%

Gravity-Based Flow Efficient Perfusion Culture System for Spheroids Mimicking Liver Inflammation

Kim,

Asif,

Chethikkattuveli Salih

et al. 2021

Biomedicines

Self Cite

View full text Add to dashboard Cite

show abstract

“…A previously validated TEER sensor was used as an additional parameter for assessing cell layer confluency in a chip platform [ 8 , 10 , 25 , 26 ]. The TEER sensor was used to assess the real-time effect of different ECMs on the barrier integrity of HepG2 cell monolayer formed in the MPS based on impedance monitoring for 144 h ( Figure 4 and Figure S9 ).…”

Section: Resultsmentioning

confidence: 99%

Extracellular Matrix Optimization for Enhanced Physiological Relevance in Hepatic Tissue-Chips

et al. 2021

Self Cite

View full text Add to dashboard Cite

The cellular microenvironment is influenced explicitly by the extracellular matrix (ECM), the main tissue support biomaterial, as a decisive factor for tissue growth patterns. The recent emergence of hepatic microphysiological systems (MPS) provide the basic physiological emulation of the human liver for drug screening. However, engineering microfluidic devices with standardized surface coatings of ECM may improve MPS-based organ-specific emulation for improved drug screening. The influence of surface coatings of different ECM types on tissue development needs to be optimized. Additionally, an intensity-based image processing tool and transepithelial electrical resistance (TEER) sensor may assist in the analysis of tissue formation capacity under the influence of different ECM types. The current study highlights the role of ECM coatings for improved tissue formation, implying the additional role of image processing and TEER sensors. We studied hepatic tissue formation under the influence of multiple concentrations of Matrigel, collagen, fibronectin, and poly-L-lysine. Based on experimental data, a mathematical model was developed, and ECM concentrations were validated for better tissue development. TEER sensor and image processing data were used to evaluate the development of a hepatic MPS for human liver physiology modeling. Image analysis data for tissue formation was further strengthened by metabolic quantification of albumin, urea, and cytochrome P450. Standardized ECM type for MPS may improve clinical relevance for modeling hepatic tissue microenvironment, and image processing possibly enhance the tissue analysis of the MPS.

show abstract

“…Alternatively, integrated TEER electrodes in OoCs have been already reported. [15][16][17][18][19][20][21] Using advanced microfabrication technologies, miniaturized sensors can be directly embedded in, or in close proximity to the cell culture channels. This was accomplished by patterning electrodes on the top and bottom substrates of the channels.…”

Section: Introductionmentioning

confidence: 99%

“…This issue has been solved by using very thin electrodes of gold 19 or indium tin oxide. 15 However, these fabrication methods are based on the sputtering of electrodes, an expensive process often requiring cleanroom access.…”

Section: Introductionmentioning

confidence: 99%

Measuring barrier function in organ-on-chips with cleanroom-free integration of multiplexable electrodes

et al. 2021

View full text Add to dashboard Cite

show abstract

Real-time sensors for live monitoring of disease and drug analysis in microfluidic model of proximal tubule

Cited by 45 publications

References 44 publications

Gravity-Based Flow Efficient Perfusion Culture System for Spheroids Mimicking Liver Inflammation

Gravity-Based Flow Efficient Perfusion Culture System for Spheroids Mimicking Liver Inflammation

Extracellular Matrix Optimization for Enhanced Physiological Relevance in Hepatic Tissue-Chips

Measuring barrier function in organ-on-chips with cleanroom-free integration of multiplexable electrodes

Contact Info

Product

Resources

About