Real-time quantitative PCR based sensitive detection and genotype discrimination of Pepino mosaic virus

Gutiérrez-Aguirre, Ion; Mehle, Nataša; Delić, Duška; Gruden, Kristina; Mumford, R. A.; Ravnikar, Maja

doi:10.1016/j.jviromet.2009.07.008

Cited by 64 publications

(33 citation statements)

References 28 publications

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“…For genotype discrimination and typing of EU, PE, CH2 and US1 PepMV genotypes, a real-time qRT-PCR method (Gutiérrez-Aguirre et al 2009) was used for the rapid screening of all PepMV positive samples originating from tomato and weed samples. Based on the results obtained from genotyping Cypriot PepMV isolates and due to the homogeneity of genotype distribution detected in the country, two isolates were (Kumar et al 2004) using the neighbour-joining algorithm.…”

mentioning

confidence: 99%

Detection, characterization and host range studies of Pepino mosaic virus in Cyprus

Papayiannis

Kokkinos²,

Alfaro-Fernández

2011

Eur J Plant Pathol

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confidence: 99%

Detection, characterization and host range studies of Pepino mosaic virus in Cyprus

Papayiannis

Kokkinos²,

Alfaro-Fernández

2011

Eur J Plant Pathol

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“…qPCR for detection of a quarantine grapevine pathogen, Xylophilus ampelinus [25], was the first qPCR-based technique included in the EPPO diagnostic protocols, and it is still in use today [26]. Other qPCR methods that have been developed at the NIB can detect plant pathogenic viruses (e.g., potato virus Y isolates, Pepino mosaic virus [27]) and bacteria (e.g., Erwinia amylovora), and Flavescence dorée and Bois noir phytoplasma in grapevine [28,29]. Recently, methods using dPCR for detection and quantification of microorganisms have been published [30,31].…”

Section: Microorganisms -Plant Pathogensmentioning

confidence: 99%

Nucleic-acid analysis in new fields of metrology

Camloh

Dreo

Gruden

et al. 2015

17th International Congress of Metrology

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Abstract. Nucleic-acid analysis (NAA) represents a key bioanalysis to which metrological approaches are applied in various fields, such as biotechnology, food safety, public health, and environmental monitoring. NAA enables detection, identification and quantification of nucleic acids of different organisms and matrices. Approaches specific for the target are most common, and real-time polymerase chain reaction (qPCR) analysis is the most frequently used technique. Other methods are being developed that intensively address specific needs. For example, digital PCR enables sensitive detection and accurate absolute quantification without comparison to reference materials, and rapid isothermal approaches provide simple target detection on-site. At the National Institute of Biology, extensive research has been carried out over the last two decades for the development and assessment of NAA techniques and their applications to different fields. Here, we present some of our experiences with the application of metrological approaches to studies of genetically modified organisms and plant pathogenic microorganisms.

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“…In recent years the number of phytopathogen detection tests based on qPCR has been increasing very fast. In our laboratory alone, we have introduced several qPCR-based assays for detection of various plant pathogens, in addition to the ones presented here , Gutiérrez-Aguirre et al 2009, Kogovšek et al 2008). …”

Section: Real-time Pcrmentioning

confidence: 99%

Real-Time PCR Detection Methods for Economically Important Grapevine Related Bacteria

Hren

Dreo

Erjavec

et al. 2010

Methodologies and Results in Grapevine Research

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Preventive measures are extremely important for control of diseases that are caused by plant pathogenic bacteria on economically important plants. For this reason, fast and reliable detection methods are required. Along with the time-consuming conventional detection methods such as isolation and culturing of bacteria on media, PCR-based methods have been introduced as supplementary tests for better diagnosis of plant pathogenic bacteria. In the case of non-culturable bacteria, such as Phytoplasmas, PCR-based tests even became indispensable. Real-time PCR has become a widely used platform in nucleic acid detection and quantification in diagnostics. Despite the general guidelines for some of the steps, there are no common guidelines or standard operating procedures for introduction of real-time PCR-based detection systems for pathogens for routine laboratory use. Four cases of detection systems for grapevine pathogenic bacteria that were developed at the National Institute of Biology are presented and discussed here, providing a practical overview of the whole process from the initial assay design to the implementation of the assay into the laboratory.

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Real-time quantitative PCR based sensitive detection and genotype discrimination of Pepino mosaic virus

Cited by 64 publications

References 28 publications

Detection, characterization and host range studies of Pepino mosaic virus in Cyprus

Detection, characterization and host range studies of Pepino mosaic virus in Cyprus

Nucleic-acid analysis in new fields of metrology

Real-Time PCR Detection Methods for Economically Important Grapevine Related Bacteria

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