Real-time quantification of viable bacteria in liquid medium using infrared thermography

Salaimeh, Ahmad; Campion, Jeffrey J.; Gharaibeh, Belal; Evans, Martin E.; Saito, Kozo

doi:10.1016/j.infrared.2011.08.004

Cited by 18 publications

(9 citation statements)

References 38 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Finally also not directly related to isothermal microcalorimetry, infrared thermography might be an interesting tool to rapidly screen for bacterial growth [138,139] especially since the methods can be applied with conventional microtiterplates that are incubated separately under ''roughly isothermal condition'' (i.e., a conventional lab incubator). The method seems to have a detection limit below 100 CFU ml À1 and seems to have strong potential.…”

Section: Technical Progress and Future Perspectivesmentioning

confidence: 99%

Microcalorimetric assays for measuring cell growth and metabolic activity: Methodology and applications

Braissant

Bachmann

Bonkat

2015

Methods

View full text Add to dashboard Cite

Section: Technical Progress and Future Perspectivesmentioning

confidence: 99%

Microcalorimetric assays for measuring cell growth and metabolic activity: Methodology and applications

Braissant

Bachmann

Bonkat

2015

Methods

View full text Add to dashboard Cite

“…In general, E s and E b are negligible compared to E t .The time constant of temperature fall from the liquid medium to the surrounding is dependent on the thermo-physical properties of the liquid growth medium and is given by the following equation Presence of bacteria in the liquid medium can be considered as an additional source of heat, which is due to the metabolic activities of the bacteria. Salaimeh et al 33 provided detailed solution of the problem under this condition and defined energy content (EC) as a dimensionless quantity which is directly proportional to the heat generated by the metabolic activities of the bacteria. 32 Energy content is defined by the following equation:…”

Section: 29mentioning

confidence: 99%

“…Salaimeh et al 33 have also used the infrared thermography in 2011 for Real-time quantification of viable bacteria in liquid medium. The approach used in their study was using IRT video, thermodynamics laws, and heat transfer mechanisms to directly measure, in real-time, the amount of energy lost as heat from the surface of a liquid sample containing bacteria when the specimen cools to a lower temperature over 2min.…”

Section: 29mentioning

confidence: 99%

“…they observed that the Energy Content (EC) of liquid media containing as few as 120 colony-forming units (CFU) of Escherichia coli per ml was significantly higher than that of sterile media (P<0.0001), and that EC and viable counts were strongly positively correlated (r=0.986) over a range of 120 to approximately 5_108CFU/ml. 33,34 Kim et al 35 used infrared lock-in thermography and photothermal wave model for the detection of mechanical damage and estimation of damage depth and also quantitative evaluation of bruises on pears. Figure 3 shows the arrangement they used schematically.…”

Section: 29mentioning

confidence: 99%

See 1 more Smart Citation

Thermography, A New Approach in Food Science Studies: A Review

Samanian

Mohebbi

2016

MOJFPT

View full text Add to dashboard Cite

Background: Thermography can be defined as a process of writing or printing involving the use of heat. It's a technique for detecting and measuring variations in the heat emitted by various regions of the body and transforming them into visible signals that can be recorded photographically. Clearly it would be of great use in diagnosing abnormal or diseased underlying conditions. But in the recent years it has been suggested as a viable method in food science research both in detection and process control. Scope and approach:We reviewed thermography as a not new method which has recently found its way into vast areas of research and practice. After discussing its history and fundamentals some practical applications of thermography such as medical, agricultural and quality control are presented. But the main focus of review is laid on its usage in food science and technology, which is provided in two main categories of process control and detection, through a series of case studies. Key findings and conclusion:Thermography is a non invasive, non destructive and instantaneous method that makes dynamic observations feasible. It also can be used to confirm any slight gradient in temperature, suggesting it as a detection method. The most important drawback to method is being highly. Modification of the method to make it into a practical procedure in industry, heat and mass transfer studies and microbiological studies can be possible future concerns of the method.

show abstract

“…Methods such as fluorescence [23], bioluminescence [3], flow cytometry [16], autofluorescence [7], real-time polymerase chain reaction [11], and infrared spectroscopy [26] are now being used to quickly quantify bacteria.…”

Section: Introductionmentioning

confidence: 99%

Evaluation of Petrifilm™ Aerobic Count Plates as an Equivalent Alternative to Drop Plating on R2A Agar Plates in a Biofilm Disinfectant Efficacy Test

et al. 2014

View full text Add to dashboard Cite

This paper compares Petrifilm™ aerobic count (AC) plates to drop plating on R2A agar plates as an alternative method for biofilm bacteria enumeration after application of a disinfectant. A Pseudomonas aeruginosa biofilm was grown in a Centers for Disease Control and Prevention biofilm reactor (ASTM E2562) and treated with 123 ppm sodium hypochlorite (as free chlorine) according to the Single Tube Method (ASTM E2871). Aliquots from the same dilution tubes were plated on Petrifilm™ AC plates and drop plated on R2A agar plates. The Petrifilm™ AC and R2A plates were incubated for 48 and 24 h, respectively, at 36 ± 1 °C. After nine experimental runs performed by two technicians, the mean difference in biofilm log densities [log biofilm density (LD) = log10(CFU/cm(2))] between the two methods for control coupons, treated coupons, and log reduction (LR) was 0.052 (p = 0.451), -0.102 (p = 0.303), and 0.152 (p = 0.313). Equivalence testing was used to assess equivalence of the two plating methods. The 90 % confidence intervals for the difference in control and treated mean LDs between methods were (-0.065, 0.170) and (-0.270, 0.064), both of which fall within a (-0.5, +0.5) equivalence criterion. The 90 % confidence interval for the mean LR difference (-0.113, 0.420) also falls within this equivalence criterion. Thus, Petrifilm™ AC plates were shown to be statistically equivalent to drop plating on R2A agar for the determination of control LDs, treated LDs, and LR values in an anti-biofilm efficacy test. These are the first published results that establish equivalency to a traditional plate counting technique for biofilms and for a disinfectant assay.

show abstract

Real-time quantification of viable bacteria in liquid medium using infrared thermography

Cited by 18 publications

References 38 publications

Microcalorimetric assays for measuring cell growth and metabolic activity: Methodology and applications

Microcalorimetric assays for measuring cell growth and metabolic activity: Methodology and applications

Thermography, A New Approach in Food Science Studies: A Review

Evaluation of Petrifilm™ Aerobic Count Plates as an Equivalent Alternative to Drop Plating on R2A Agar Plates in a Biofilm Disinfectant Efficacy Test

Contact Info

Product

Resources

About