Real time qPCR expression analysis of some stress related genes in leaf tissue of Pyrus communis cv. Conférence after infection with Erwinia amylovora

Vrancken, Kristof; Schoofs, H.; Deckers, T.; Valcke, Roland

doi:10.1007/s00468-011-0666-3

Cited by 6 publications

(3 citation statements)

References 33 publications

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“…Research done by our lab showed that the transcription patterns of two key genes, anthocyanidin reductase (ANR) and chalcone synthase (CHS), related to this phenylpropanoid-flavonoid pathway, considerately increased in E. amylovora-inoculated mature leaves compared with the control and mockinoculated mature leaves of the pear cultivar 'Conference', with the strongest reaction 48 h after inoculation (Vrancken et al, 2012). These effects of E. amylovora were also visualized in histological sections, and confirmed by HPLC, as epicatechin, which is produced from cyanidin via ANR, increased 72 h after inoculation in E. amylovora-inoculated mature leaf tissue.…”

Section: Secondary Metabolitesmentioning

confidence: 99%

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Pathogenicity and infection strategies of the fire blight pathogen Erwinia amylovora in Rosaceae: State of the art

Vrancken

Holtappels

Schoofs³

et al. 2013

Microbiology

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Plants are host to a large amount of pathogenic bacteria. Fire blight, caused by the bacterium Erwinia amylovora, is an important disease in Rosaceae. Pathogenicity of E. amylovora is greatly influenced by the production of exopolysaccharides, such as amylovoran, and the use of the type III secretion system, which enables bacteria to penetrate host tissue and cause disease. When infection takes place, plants have to rely on the ability of each cell to recognize the pathogen and the signals emanating from the infection site in order to generate several defence mechanisms. These mechanisms consist of physical barriers and the production of antimicrobial components, both in a preformed and an inducible manner. Inducible defence responses are activated upon the recognition of elicitor molecules by plant cell receptors, either derived from invading microorganisms or from pathogen-induced degradation of plant tissue. This recognition event triggers a signal transduction cascade, leading to a range of defence responses [reactive oxygen species (ROS), plant hormones, secondary metabolites, ...] and redeployment of cellular energy in a fast, efficient and multiresponsive manner, which prevents further pathogen ingress. This review highlights the research that has been performed during recent years regarding this specific plantpathogen interaction between Erwinia amylovora and Rosaceae, with a special emphasis on the pathogenicity and the infection strategy of E. amylovora and the possible defence mechanisms of the plant against this disease.

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Section: Secondary Metabolitesmentioning

confidence: 99%

“…Specific balances in antioxidative genes countering these ROS could explain why ontogenesis-related differences exist between cultivars regarding fire blight susceptibility (Vrancken et al, 2012).…”

Section: Ros and The Generation Of An Oxidative Burstmentioning

confidence: 99%

Pathogenicity and infection strategies of the fire blight pathogen Erwinia amylovora in Rosaceae: State of the art

Vrancken

Holtappels

Schoofs³

et al. 2013

Microbiology

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“…APX was involved in different antioxidant responses to bacterial infection in young and old leaves of Pyrus communis cv. Conference [189]. AsA participated in the dynamic antioxidant response of Norway spruce phloem attacked by Ips typographus L. [190].…”

Section: The Involvement Of Asc In the Response To Biotic Stressmentioning

confidence: 99%

Ascorbic Acid—The Little-Known Antioxidant in Woody Plants

et al. 2019

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Reactive oxygen species (ROS) are constantly produced by metabolically active plant cells. The concentration of ROS may determine their role, e.g., they may participate in signal transduction or cause oxidative damage to various cellular components. To ensure cellular homeostasis and minimize the negative effects of excess ROS, plant cells have evolved a complex antioxidant system, which includes ascorbic acid (AsA). AsA is a multifunctional metabolite with strong reducing properties that allows the neutralization of ROS and the reduction of molecules oxidized by ROS in cooperation with glutathione in the Foyer-Halliwell-Asada cycle. Antioxidant enzymes involved in AsA oxidation and reduction switches evolved uniquely in plants. Most experiments concerning the role of AsA have been performed on herbaceous plants. In addition to extending our understanding of this role in additional taxa, fundamental knowledge of the complex life cycle stages of woody plants, including their development and response to environmental factors, will enhance their breeding and amend their protection. Thus, the role of AsA in woody plants compared to that in nonwoody plants is the focus of this paper. The role of AsA in woody plants has been studied for nearly 20 years. Studies have demonstrated that AsA is important for the growth and development of woody plants. Substantial changes in AsA levels, as well as reduction and oxidation switches, have been reported in various physiological processes and transitions described mainly in leaves, fruits, buds, and seeds. Evidently, AsA exhibits a dual role in the photoprotection of the photosynthetic apparatus in woody plants, which are the most important scavengers of ozone. AsA is associated with proper seed production and, thus, woody plant reproduction. Similarly, an important function of AsA is described under drought, salinity, temperature, light stress, and biotic stress. This report emphasizes the involvement of AsA in the ecological advantages, such as nutrition recycling due to leaf senescence, of trees and shrubs compared to nonwoody plants.

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Phylogenetic analysis of PR genes in some pome fruit species with the emphasis on transcriptional analysis and ROS response under Erwinia amylovora inoculation in apple

et al. 2015

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Attempts were made to identify eight pathogenesis related (PR) genes (i.e., PR-1a, PR3-ch1, PR3-Ch2, PR3-Ch3, PR3-Ch4, PR3-Ch5, PR-5 and PR-8) from 27 genotypes of apple, quince and pear, which are induced in response to inoculation with the pathogen Erwinia amylovora, the causal agent of fire blight. Totally, 32 PR genes of different families were obtained, excepting PR3-Ch2 (amplified only in apple) and PR3-Ch4 (amplified only in apple and pear), the others were successfully amplified in all the genotypes of apple, quince and pear. Evolutionary, the genes of each family exhibited significant homology with each other, as the corresponded phylogenetic neighbor-joining-based dendrograms were taken into consideration. Meanwhile, according to the expression assay, it was deduced that the pathogen activity can significantly affect the expression levels of some selected PR genes of PR3-Ch2, PR3-Ch4, PR3-Ch5 and particularly Cat I in both resistant (MM-111) and semi-susceptible (MM-106) apple rootstocks. Lastly, it was concluded that the pathogen E. amylovora is able to stimulate ROS response, particularly using generation of hydrogen peroxide (H2O2) in both aforementioned apple rootstock.

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Real time qPCR expression analysis of some stress related genes in leaf tissue of Pyrus communis cv. Conférence after infection with Erwinia amylovora

Cited by 6 publications

References 33 publications

Pathogenicity and infection strategies of the fire blight pathogen Erwinia amylovora in Rosaceae: State of the art

Pathogenicity and infection strategies of the fire blight pathogen Erwinia amylovora in Rosaceae: State of the art

Ascorbic Acid—The Little-Known Antioxidant in Woody Plants

Phylogenetic analysis of PR genes in some pome fruit species with the emphasis on transcriptional analysis and ROS response under Erwinia amylovora inoculation in apple

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