Real-time, profile-corrected single snapshot imaging of optical properties

Giessen, Martijn van de; Angelo, Joseph; Gioux, Sylvain

doi:10.1364/boe.6.004051

Cited by 61 publications

(71 citation statements)

References 27 publications

(42 reference statements)

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“…This technique relies on the projection of a single 1D-sinusoidal illumination pattern that is then imaged and decomposed into components of AC, DC, and phase for further processing in the spatial frequency domain [11,15,20]. Calibration at multiple distances is done to calculate optical property and 3D profile maps and to also account for system parameter variations due to changes in the sample distance.…”

Section: Discussionmentioning

confidence: 99%

“…Our lab recently developed a novel spatial frequency domain technique called single snapshot of optical properties (SSOP) that allows for video-rate acquisition of optical properties [11,20]. Long acquisition times (fewer than 1 frame per second, fps) are prone to motion artifacts created by a moving sample and are unable to capture dynamics of motion or changing optical properties.…”

Section: Single Snapshot Of Optical Properties Imagingmentioning

confidence: 99%

“…Profile corrections are absolutely crucial for both SFDI [16,17] and SSOP [20] as varying sample height causes uncorrected measurements of absorption and reduced scattering properties. Hence, this work also implements and demonstrates the need for a height-based correction.…”

Section: Endoscopic Ssopmentioning

confidence: 99%

“…projected spatial frequency and the radiance of source intensity. However, each of these parameters can be calibrated and accounted for over a range of distances by using the same principles demonstrated previously [16,20]. For any given parameter, calibration measurements on a reference phantom are made at several known distances from the endoscope and the parameter is fit to a regression as a function of distance.…”

Section: Endoscopic Ssopmentioning

confidence: 99%

“…The single exposure acquisition and simple instrumentation of SSOP makes it amenable to the demanding environment of endoscopic imaging. Moreover, recent work has demonstrated the ability to extract phase information from a single projection while using SSOP, enabling height-corrections that accommodate for irradiance loss due to variations in surface topography [20]. These features have led to this study that aims to provide quantitative endoscopic imaging.…”

Section: Introductionmentioning

confidence: 99%

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Real-time endoscopic optical properties imaging

Angelo¹,

Giessen²,

Gioux³

2017

Biomed. Opt. Express

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Abstract:With almost 50% of all surgeries in the U.S. being performed as minimally invasive procedures, there is a need to develop quantitative endoscopic imaging techniques to aid surgical guidance. Recent developments in widefield optical imaging make endoscopic implementations of real-time measurement possible. In this work, we introduce a proof-ofconcept endoscopic implementation of a functional widefield imaging technique called 3D single snapshot of optical properties (3D-SSOP) that provides quantitative maps of absorption and reduced scattering optical properties as well as surface topography with simple instrumentation added to a commercial endoscope. The system's precision and accuracy is validated using tissue-mimicking phantoms, showing a max error of 0.004 mm −1 , 0.05 mm −1 , and 1.1 mm for absorption, reduced scattering, and sample topography, respectively. This study further demonstrates video acquisition of a moving phantom and an in vivo sample with a framerate of approximately 11 frames per second.

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Section: Discussionmentioning

confidence: 99%

Section: Single Snapshot Of Optical Properties Imagingmentioning

confidence: 99%

Section: Endoscopic Ssopmentioning

confidence: 99%

Section: Endoscopic Ssopmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Real-time endoscopic optical properties imaging

Angelo¹,

Giessen²,

Gioux³

2017

Biomed. Opt. Express

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Multiparameter wide‐field integrated optical imaging system‐based spatially modulated illumination and laser speckles in model of tissue injuries

Bloygrund

Franjy-Tal

Rosenzweig

et al. 2019

Journal of Biophotonics

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In this report, an integrated optical platform based on spatial illumination together with laser speckle contrast technique was utilized to measure multiple parameters in live tissue including absorption, scattering, saturation, composition, metabolism, and blood flow. Measurements in three models of tissue injury including drug toxicity, artery occlusion, and acute hyperglycemia were used to test the efficacy of this system. With this hybrid apparatus, a series of structured light patterns at low and high spatial frequencies are projected onto the tissue surface and diffuse reflected light is captured by a CCD camera. A six position filter wheel, equipped with four bandpass filters centered at wavelengths of 650, 690, 800 and 880 nm is placed in front of the camera. Then, light patterns are blocked and a laser source at 650 nm illuminates the tissue while the diffusely reflected light is captured by the camera through the two remaining open holes in the wheel. In this manner, near‐infrared (NIR) and laser speckle images are captured and stored together in the computer for off‐line processing to reconstruct the tissue's properties. Spatial patterns are used to differentiate the effects of tissue scattering from those of absorption, allowing accurate quantification of tissue hemodynamics and morphology, while a coherent light source is used to study blood flow changes, a feature which cannot be measured with the NIR structured light. This combined configuration utilizes the strengths of each system in a complementary way, thus collecting a larger range of sample properties. In addition, once the flow and hemodynamics are measured, tissue oxygen metabolism can be calculated, a property which cannot be measured independently. Therefore, this merged platform can be considered a multiparameter wide‐field imaging and spectroscopy modality. Overall, experiments demonstrate the capability of this spatially coregistered imaging setup to provide complementary, useful information of various tissue metrics in a simple and noncontact manner, making it attractive for use in a variety of biomedical applications.

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Noninvasive detection of cancer spread to lymph nodes: A review of molecular imaging principles and protocols

Torres

Tichauer

2018

Journal of Surgical Oncology

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Identification of cancer spread to tumor-draining lymph nodes offers critical information for guiding treatment in many cancer types. Current clinical methods of nodal staging are invasive and can have substantial negative side effects. Molecular imaging protocols have long been proposed as a less invasive means of nodal staging, having the potential to enable highly sensitive and specific evaluations. This review article summarizes the current status and future perspectives for molecular targeted nodal staging.

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Real-time, profile-corrected single snapshot imaging of optical properties

Cited by 61 publications

References 27 publications

Real-time endoscopic optical properties imaging

Real-time endoscopic optical properties imaging

Multiparameter wide‐field integrated optical imaging system‐based spatially modulated illumination and laser speckles in model of tissue injuries

Noninvasive detection of cancer spread to lymph nodes: A review of molecular imaging principles and protocols

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