Real-time point-of-care total protein measurement with a miniaturized optoelectronic biosensor and fast fluorescence-based assay

Mahzabeen, Fariah; Vermesh, Ophir; Levi, Jelena; Tan, Marilyn; Alam, Israt S.; Chan, Carmel T.; Gambhir, Sanjiv S.; Harris, James S.

doi:10.1016/j.bios.2020.112823

Cited by 13 publications

(7 citation statements)

References 35 publications

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“…On the White FOx 1.0, data were collected by FOx software (FOx Biosystems), and analyzed to generate the calibration curves. The fitting was performed with non-linear regression (y=Ax/(B+x)) whereas the limits of detection (LOD), and the coefficients of variation (CV) were calculated as previously reported (Lu et al, 2017;Mahzabeen et al, 2021). The calibration curves were plotted by programming in Matlab 2019b (The MathWorks Inc., USA).…”

Section: Discussionmentioning

confidence: 99%

Point-of-care therapeutic drug monitoring of adalimumab by integrating a FO-SPR biosensor in a self-powered microfluidic cartridge

Ordutowski

Tricht

et al. 2022

Biosensors and Bioelectronics

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Section: Discussionmentioning

confidence: 99%

Point-of-care therapeutic drug monitoring of adalimumab by integrating a FO-SPR biosensor in a self-powered microfluidic cartridge

Ordutowski

Tricht

et al. 2022

Biosensors and Bioelectronics

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“…From an applicative perspective, the utilisation of PIFE in various emerging platforms to tackle previously unexplored problems is experiencing a clear surge. These platforms encompass a wide range of innovative techniques, such as smPIFE with continuous repositioning of single molecules within an anti-Brownian electrokinetic trap [111], the integration of smPIFE with force spectroscopy [112], the application of PIFE for investigating liquid-liquid phase separation of the RNA-binding protein FUsed in Sarcoma (FUS) [113], as well as bio-detection and sensing applications [114,115], including the introduction of aptamer-based PIFE [116,117]. Undoubtedly, this surge in the adoption of smPIFE points towards a promising future of PIFE in the realms of biomolecular science and biomedical application development.…”

Section: Pife: What's Next?mentioning

confidence: 99%

A new twist on PIFE: photoisomerisation-related fluorescence enhancement

Ploetz,

Ambrose,

Barth

et al. 2023

Methods Appl. Fluoresc.

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PIFE was first used as an acronym for protein-induced fluorescence enhancement, which refers to the increase in fluorescence observed upon the interaction of a fluorophore, such as a cyanine, with a protein. This fluorescence enhancement is due to changes in the rate of cis/trans photoisomerisation. It is clear now that this mechanism is generally applicable to interactions with any biomolecule and, in this review, we propose that PIFE is thereby renamed according to its fundamental working principle as photoisomerisation-related fluorescence enhancement, keeping the PIFE acronym intact. We discuss the photochemistry of cyanine fluorophores, the mechanism of PIFE, its advantages and limitations, and recent approaches to turn PIFE into a quantitative assay. We provide an overview of its current applications to different biomolecules and discuss potential future uses, including the study of protein-protein interactions, protein-ligand interactions and conformational changes in biomolecules.

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“…Some devices are capable of performing several tests at once or storing and recalling test results for further analysis. The devices are commonly used for medical monitoring applications for measurements of analytes such as hormones, as well as point-of-care testing of various infections [22][23][24] and diseases like HIV [25], malaria [26], and diabetes [27].…”

Section: Working Principlementioning

confidence: 99%

Emerging Technologies for Fluorescence-Based Optical Test Strip Readers

AKSOY

DULDA

Ertas

2023

EJOSAT

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Fluorescence-based optical test strip readers are used to detect and quantify fluorescent signals from immunoassay test strips in medicine, especially for point-of-care applications. The design of optical systems including light sources and detection systems in these devices is not only indispensable but also the most critical part for specific detection applications. This study aims to provide detailed information about fluorescence-based optical test strip readers, existing and emerging technologies, and their contributions to the design of the device. The most commonly used technologies of light sources and detection systems have been discussed and compared for the ideal design. Arc and Xenon lamps may not be appropriate for portable and low-cost devices as they are larger and more costly when compared to LEDs and laser diodes. Photodiodes and CMOS detectors can be used for the design of low-cost, portable fluorescence-based optical test strip readers as they are cheaper and smaller in size when compared to CCDs and PMTs. Both light source and detector should be chosen according to the application priorities and spectral characteristics of the fluorescent molecule by integrating them with proper optical elements like filters, mirrors, etc. This study contributes to the people who are interested in the design of fluorescence-based optical test strip readers as it serves as a guideline for the optical test strip reader systems.

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Real-time point-of-care total protein measurement with a miniaturized optoelectronic biosensor and fast fluorescence-based assay

Cited by 13 publications

References 35 publications

Point-of-care therapeutic drug monitoring of adalimumab by integrating a FO-SPR biosensor in a self-powered microfluidic cartridge

Point-of-care therapeutic drug monitoring of adalimumab by integrating a FO-SPR biosensor in a self-powered microfluidic cartridge

A new twist on PIFE: photoisomerisation-related fluorescence enhancement

Emerging Technologies for Fluorescence-Based Optical Test Strip Readers

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