Real-time phase-resolved functional optical coherence tomography by use of optical Hilbert transformation

Zhao, Yonghua; Chen, Zhongping; Ding, Zhihua; Ren, Haoran; Nelson, J. Stuart

doi:10.1364/ol.27.000098

Cited by 87 publications

(56 citation statements)

References 13 publications

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“…Phase-resolved OCT (PR-OCT) was initially proposed for measuring the velocity of blood flow by evaluating the phase shift between adjacent OCT A-scan signals [21]. This concept was first applied to characterize tissue Doppler resulting from soft tissue motion in 2006 [11].…”

Section: Introductionmentioning

confidence: 99%

Pulsatile motion of the trabecular meshwork in healthy human subjects quantified by phase-sensitive optical coherence tomography

Shen

Johnstone

et al. 2013

Biomed. Opt. Express

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Aqueous leaves the anterior chamber of eye by passing through the trabecular meshwork (TM), a tissue thought to be responsible for increased outflow resistance in glaucoma. Motion assessment could permit characterization of TM biomechanical properties necessary to maintain intra-ocular pressure (IOP) within a narrow homeostatic range. In this paper, we report the first in vivo identification of TM motion in humans. We use a phase-sensitive optical coherence tomography (PhS-OCT) system with sub-nanometer sensitivity to detect and image dynamic pulse-induced TM motion. To permit quantification of TM motion and relationships we develop and apply a phase compensation algorithm permitting removal of the otherwise evitable confounding effects of bulk motion. Twenty healthy human eyes from 10 subjects are imaged. The results permit visualization of pulsatile TM motion visualization by PhS-OCT; correlation with the digital/cardiac pulse is highly significant. The correlation permits assessment of the phase lag and time delay between TM motion and the cardiac pulse. In this study, we find that the digital pulse leads the pulsatile TM motion by a mean phase of 3.53 ± 0.48 rad and a mean time of 0.5 ± 0.14 s in the fundamental frequency. A significant linear relationship is present between the TM phase lag and the heart rate (p value < 0.05). The TM phase lag is also affected by age, the relationship not quite reaching significance in the current study. PhS-OCT reveals pulse-induced motion of the TM that may provide insights into the biomechanics of the tissues involved in the regulation of IOP. 11052-11065 (2008). 14. X. Liang, S. G. Adie, R. John, and S. A. Boppart, "Dynamic spectral-domain optical coherence elastography for tissue characterization," Opt. Express 18(13), 14183-14190 (2010). 15. P. Li, A. Liu, L. Shi, X. Yin, S. Rugonyi, and R. K. Wang, "Assessment of strain and strain rate in embryonic chick heart in vivo using tissue Doppler optical coherence tomography," Phys. Med. Biol. 56 (22), 7081-7092 (2011). 16. P. Li, X. Yin, L. Shi, S. Rugonyi, and R. K. Wang, "In vivo functional imaging of blood flow and wall strain rate in outflow tract of embryonic chick heart using ultrafast spectral domain optical coherence tomography," J. ©2013 Optical Society of America

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Section: Introductionmentioning

confidence: 99%

Pulsatile motion of the trabecular meshwork in healthy human subjects quantified by phase-sensitive optical coherence tomography

Shen

Johnstone

et al. 2013

Biomed. Opt. Express

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“…In order to obtain additional biological information of tissue, many functional SDOCTs have been developed. Thanks to the advantages of the improved imaging speed and sensitivity in SDOCT, a functional extension of SDOCT, spectral domain phase resolved Doppler OCT (PRDOCT) [3,4], which relies on the phase difference between successive A-lines at the same depth, was developed to extract velocity information of blood flow in functional vessels within the scanned tissue beds. To optimize the performance of PRDOCT, Ren et al proposed a moving-scatterer-sensitivity optical Doppler tomography (MSS-ODT) technique to improve sensitivity for imaging blood flow in vivo [5], and Wang and An also proposed a Doppler optical micro-angiography (DOMAG) method to remove the characteristic texture pattern noise caused by the heterogeneous property of sample [6].…”

Section: Introductionmentioning

confidence: 99%

The cross-correlation in spectral domain based Doppler optical coherence tomography

Chen

Liao

Gao

2015

Optics Communications

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“…[25][26][27] When combined with the Doppler effect of flowing particles on the frequency of the light, OCT has been extended to include imaging of blood flow in tissue. The most commonly used OCT method for measuring blood flow is the phase-resolved Doppler OCT. 28,29 Subsequently, OMAG was combined with phaseresolved Doppler OCT to produce a method called Doppler OMAG (DOMAG), which has extended OMAG's capabilities to quantitatively measure blood flow velocity in the axial direction. 30 DOMAG has a higher signal-to-noise ratio and a higher performance for velocity estimation compared to phase-resolved Doppler OCT. 30 In the current study we used DOMAG to examine changes in CoBF in mice anesthetized with isoflurane and subjected to decreased oxygen concentration to cause severe systemic hypoxia (SpO 2 ∼ 80%).…”

Section: Introductionmentioning

confidence: 99%

Effects of hypoxia on cochlear blood flow in mice evaluated using Doppler optical microangiography

et al. 2012

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Abstract. Reduced cochlear blood flow (CoBF) is a main contributor to hearing loss. Studying CoBF has remained a challenge due to the lack of available tools. Doppler optical microangiography (DOMAG), a method to quantify single-vessel absolute blood flow, and laser Doppler flowmetry (LDF), a method for measuring the relative blood flow within a large volume of tissue, were used for determining the changes in CoBF due to systemic hypoxia in mice. DOMAG determined the change in blood flow in the apical turn (AT) with single-vessel resolution, while LDF averaged the change in the blood flow within a large volume of the cochlea (hemisphere with ∼1 to 1.5 mm radius). Hypoxia was induced by decreasing the concentration of oxygen-inspired gas, so that the oxygen saturation was reduced from >95% to ∼80%. DOMAG determined that during hypoxia the blood flow in two areas of the AT near and far from the helicotrema were increased and decreased, respectively. The LDF detected a decrease in blood flow within a larger volume of the cochlea (several turns averaged together). Therefore, the use of DOMAG as a tool for studying cochlear blood flow due to its ability to determine absolute flow values with single-vessel resolution was proposed.

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Real-time phase-resolved functional optical coherence tomography by use of optical Hilbert transformation

Cited by 87 publications

References 13 publications

Pulsatile motion of the trabecular meshwork in healthy human subjects quantified by phase-sensitive optical coherence tomography

Pulsatile motion of the trabecular meshwork in healthy human subjects quantified by phase-sensitive optical coherence tomography

The cross-correlation in spectral domain based Doppler optical coherence tomography

Effects of hypoxia on cochlear blood flow in mice evaluated using Doppler optical microangiography

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