Real-Time PCR to Phenotype Resistance to the Citrus Nematode Tylenchulus semipenetrans Cobb.

Ruiz, Marta; Vo, Annie Du; Becker, J. Ole; Roose, Mikeal L.

doi:10.3390/plants12132543

Cited by 2 publications

(1 citation statement)

References 34 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…For example, development of automated image processing for high-throughput nematode counting could accurately and rapidly function for high population levels of BCAs, e.g., entomopathogenic nematodes and other microorganisms [ 12 ]. Also, a new technique to extract females of Tylenchulus semipenetrans suitable for molecular studies from citrus roots may boost the chances of designating rootstocks with more desired traits [ 27 ]. Using molecular techniques and automated workflows in the above-mentioned nematode processes with precision and pace as good as or better than classical methods has been established and should be expanded for better achievements.…”

Section: Sound Sampling and Extraction Methods As Prerequisites For T...mentioning

confidence: 99%

Upgrading Strategies for Managing Nematode Pests on Profitable Crops

Abd-Elgawad

2024

Plants

View full text Add to dashboard Cite

Plant-parasitic nematodes (PPNs) reduce the high profitability of many crops and degrade their quantitative and qualitative yields globally. Traditional nematicides and other nematode control methods are being used against PPNs. However, stakeholders are searching for more sustainable and effective alternatives with limited side effects on the environment and mankind to face increased food demand, unfavorable climate change, and using unhealthy nematicides. This review focuses on upgrading the pre-procedures of PPN control as well as novel measures for their effective and durable management strategies on economically important crops. Sound and effective sampling, extraction, identification, and counting methods of PPNs and their related microorganisms, in addition to perfecting designation of nematode–host susceptibility/resistance, form the bases for these strategies. Therefore, their related frontiers should be expanded to synthesize innovative integrated solutions for these strategies. The latter involve supplanting unsafe nematicides with a new generation of safe and reliable chemical nematicidal and bionematicidal alternatives. For better efficacy, nematicidal materials and techniques should be further developed via computer-aided nematicide design. Bioinformatics devices can reinforce the potential of safe and effective biocontrol agents (BCAs) and their active components. They can delineate the interactions of bionematicides with their targeted PPN species and tackle complex diseases. Also, the functional plan of nematicides based on a blueprint of the intended goals should be further explored. Such goals can currently engage succinate dehydrogenase, acetylcholinesterase, and chitin deacetylase. Nonetheless, other biochemical compounds as novel targets for nematicides should be earnestly sought. Commonly used nematicides should be further tested for synergistic or additive function and be optimized via novel sequential, dual-purpose, and co-application of agricultural inputs, especially in integrated pest management schemes. Future directions and research priorities should address this novelty. Meanwhile, emerging bioactivated nematicides that offer reliability and nematode selectivity should be advanced for their favorable large-scale synthesis. Recent technological means should intervene to prevail over nematicide-related limitations. Nanoencapsulation can challenge production costs, effectiveness, and manufacturing defects of some nematicides. Recent progress in studying molecular plant–nematode interaction mechanisms can be further exploited for novel PPN control given related topics such as interfering RNA techniques, RNA-Seq in BCA development, and targeted genome editing. A few recent materials/techniques for control of PPNs in durable agroecosystems via decision support tools and decision support systems are addressed. The capability and effectiveness of nematicide operation harmony should be optimized via employing proper cooperative mechanisms among all partners.

show abstract

Section: Sound Sampling and Extraction Methods As Prerequisites For T...mentioning

confidence: 99%