Real-time PCR quantification of bacterial adhesion to Caco-2 cells: Competition between bifidobacteria and enteropathogens

Evaluation of probiotic properties of Pediococcus acidilactici B14 in association with Lactobacillus acidophilus ATCC 4356 for application in a soy based aerated symbiotic dessert

“…Considering the similarity of origin between the Caco-2, HT-29 and HRT-18 strains, and the lack of reports on the use of the HRT-18 strain, data on the adhesion of LAB in Caco-2 cells were used for comparison. The adhesion capacity of P. acidilactici B14 in HRT-18 cells was considered as absent when using the standard of no adhesiveness (<5 bacteria/ Caco-2 cell) (Candela et al 2005). However, considering the parameters proposed by Del Re et al (2000), who evaluated the adhesive capacity of strains of B. longum and defined 'strong' as adhesive capacity over 40 bacteria/100 Caco-2 cells, then the P. acidilactici B14 strain showed strong adhesive capacity.…”

The Role of Diet, Prebiotic and Probiotic in the Development and Management of Inflammatory Bowel Diseases (IBD)

“…Some probiotic have the ability to block the intestinal mucosal receptor, thus prevent adhesion of pathogenic bacteria. Several strains of Lactobacilli and Bifidobacteria are able to compete with many pathogenic bacteria such as Bacteroides vulgates, Enterobacter aerogene, Listeria monocytogenes, Staphylococcus aureus, Salmonella enterica, enterotoxigenic E. coli and enteropathogenic E. coli for intestinal epithelial cell binding (Collado et al, 2007;Candela et al, 2005;Roselli et al, 2006;Sherman et al, 2005). Displacement of pathogenic bacteria can also occur even if the pathogen have attached to intestinal epithelial cells prior to prebiotic treatment (Collado et al, 2007;Candela et al, 2005).…”

Tumor Necrosis Factor Alpha Modulates the Dynamics of the Plasminogen-Mediated Early Interaction between Bifidobacterium animalis subsp. lactis and Human Enterocytes

“…B. animalis subsp. lactis BI07 adhesion to HT29 monolayers was measured by quantitative PCR (qPCR) as reported by Candela et al (9). For each experimental condition, six independent replica experiments were performed.…”

“…Consequently, in order to investigate the role of human Plg in the B. animalis subsp. lactis BI07-enterocyte interaction process, bacterial cells were preincubated with human Plg (Sigma) as reported by Attali et al (2) and then subjected to a conventional HT29 cell adhesion assay (9). Thirty-minute preincubation at 37°C with 100 g/ml human Plg significantly enhanced B. animalis subsp.…”

“…For comparison and as a control, the internalization of Salmonella enterica serovar Typhimurium by HT29 cells was determined using a gentamicin protection assay (13). S. Typhimurium, provided by A. Essig (Department of Medical Microbiology, University of Ulm, Germany), was cultured as reported by Candela et al (9). In accordance with data reported by Hess et al (13), we determined a HT29 cell internalization value of (9.32 Ϯ 1.58) ϫ 10 4 CFU of S. Typhimurium after 1 h of incubation with 1 ϫ 10 8 CFU of this microorganism.…”