Real-time PCR investigation of the prevalence of Fusobacterium necrophorum in patients with pharyngitis in Japan

Hayakawa, Kayoko; Nagashima, Maki; Kanehisa, Eriko; Watanabe, Riri; Kunimatsu, Junwa; Ohta, Kaichi; Mutoh, Yoshikazu; Hashimoto, Takehiro; Katanami, Yuichi; Yamamoto, Kei; Kutsuna, Satoshi; Tayama, Niro; Ohmagari, Norio

doi:10.1016/j.jiac.2018.09.008

Cited by 11 publications

(5 citation statements)

References 12 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Title and abstract screening identified 163 potentially relevant records of which 15 were included in the review (Figure 1). Of 15 included studies, eight evaluated McIsaac score (30)(31)(32)(33)(34)(35)(36)(37) and seven Centor score (38)(39)(40)(41)(42)(43). No studies evaluated both scores.…”

Section: Resultsmentioning

confidence: 99%

“…Ten studies used both RADTs and throat cultures (30-35, 38, 39, 42, 43) as the reference standard: throat culture performed depending on RADT results (32, 35, 38, 43), or both tests performed (30, 31, 33, 34, 39, 42). Two studies (36, 41) only used RADT, and two studies (37, 40) only used throat cultures as the reference standard. Across Centor studies there were 7400 patients in total, of which Rich et al(43) and Rimoin and colleagues (44) contributed 3963 and 2472 patients respectively.…”

Section: Resultsmentioning

confidence: 99%

“…Studies included both adults and children and also used different age ranges to classify children and adults – e.g. Hayakawa et al (40) defined adults as those aged 16 or above, while El-Ghany et al (30) defined adults as being over 18 years old.…”

Section: Resultsmentioning

confidence: 99%

See 2 more Smart Citations

Systematic review and meta-analysis of the accuracy of McIsaac and Centor score in patients presenting to secondary care with Pharyngitis

Kanagasabai

Evans

Jones

et al. 2023

Preprint

View full text Add to dashboard Cite

Background Centor and modified Centor (McIsaac) scores are clinical prediction rules used to diagnose group A streptococcus infection in patients with pharyngitis. They aim to identify the patients most likely to benefit from antibiotic treatment and reduce unnecessary antibiotic prescribing. Objectives To systematically review the literature on the diagnostic accuracy of McIsaac and Centor, and produce pooled estimates of accuracy at each score threshold, in patients presenting with acute pharyngitis to secondary care. Data sources MEDLINE, Embase and Web of science were searched from inception to June 2021. Eligibility criteria Studies that included patients who presented with acute pharyngitis to hospital emergency departments and outpatient clinics, reported McIsaac or Centor scores, and used throat cultures and/or rapid antigen detection tests as the reference standard. Review methods The review protocol was registered on PROSPERO (CRD42021267413). Study selection was performed by two reviewers independently and risk of bias was assessed using the QUADAS-2 tool. Sensitivities and specificities of McIsaac and Centor scores were pooled at each threshold using bivariate random effects meta-analysis. Results The McIsaac score had higher estimated sensitivity and lower specificity relative to Centor scores at equivalent thresholds, but with wide and overlapping confidence regions. Using either score as a triage to rapid antigen detection tests (RADT) to decide antibiotic treatment would reduce antibiotic prescription to non-GAS pharyngitis patients relative to RADT test for everyone, but also reduce antibiotic prescription to GAS patients . Conclusion Our findings suggest that high thresholds of either score excludes a proportion of true positive patients from potentially beneficial treatment. The use of a low threshold before a RADT test would reduce antibiotic prescription relative to prescribing based on score only but the economics and clinical effectiveness of this combination strategy needs assessment. We recommend continued use of existing antibiotic prescribing guidelines and patient safety netting.

show abstract

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

See 1 more Smart Citation

Systematic review and meta-analysis of the accuracy of McIsaac and Centor score in patients presenting to secondary care with Pharyngitis

Kanagasabai

Evans

Jones

et al. 2023

Preprint

View full text Add to dashboard Cite

show abstract

“…In contrast, BALF-NGS was able to rapidly detect F. necrophorum and establish the diagnosis, highlighting the advantage of this technique when cultures fail. PCR is also an important tool for Fusobacterium detection [ 14 , 15 ], but its application is limited. In recent years, high-throughput gene sequencing (NGS) has been widely applied in infectious disease diagnosis.…”

Section: Discussionmentioning

confidence: 99%

The first case report: diagnosis and management of necrotizing fusobacterium lung abscess via BALF next-generation sequencing

Liu,

Li,

et al. 2024

BMC Infect Dis

View full text Add to dashboard Cite

Background Fusobacterium necrophorum (F. necrophorum)-induced necrotizing pneumonia is a rare but severe pulmonary infection. Insufficient microbiological detection methods can lead to diagnostic difficulties. Methods We report a case of F. necrophorum lung abscess diagnosed by next-generation sequencing (NGS) of bronchoalveolar lavage fluid (BALF). Results BALF-NGS detected F. necrophorum, guiding subsequent targeted antibiotic therapy. With active drainage and metronidazole treatment, the patient’s condition was effectively treated. Conclusion BALF-NGS is a valuable tool for the rapid diagnosis of infections caused by difficult-to-culture bacteria. It played a decisive role in the early identification of F. necrophorum, enabling timely and targeted antibiotic intervention. Early diagnosis and appropriate treatment are crucial for the management of F. necrophorum pneumonia.

show abstract

“…Of the six studies included in the qualitative analysis, three were of overall high quality and one of medium quality [62][63][64][65] (figure 2), and these were included in the meta-analysis.…”

Section: Methodological Qualitymentioning

confidence: 99%

Acute sore throat and Fusobacterium necrophorum in primary healthcare: a systematic review and meta-analysis

et al. 2021

View full text Add to dashboard Cite

PurposeThe main objective of this review was to describe and quantify the association between Fusobacterium necrophorum (FN) and acute sore throat in primary healthcare (PHC).MethodsIn this systematic review and meta-analysis, we searched Scopus and PubMed for case–control studies reporting the prevalence of FN in patients attending primary care for an uncomplicated acute sore throat as well as in healthy controls. Only studies published in English were considered. Publications were not included if they were case studies, or if they included patients prescribed antibiotics before the throat swab, patients with a concurrent malignant disease, on immunosuppression, having an HIV infection, or patients having another acute infection in addition to a sore throat. Inclusion criteria and methods were specified in advance and published in PROSPERO. The primary outcome was positive etiologic predictive value (P-EPV), quantifying the probability for an association between acute sore throat and findings of FN in the pharynx. For comparison, our secondary outcome was the corresponding P-EPV for group A Streptococcus (GAS).ResultsPubMed and Scopus yielded 258 and 232 studies, respectively. Removing duplicates and screening the abstracts resulted in 53 studies subsequently read in full text. For the four studies of medium to high quality included in the meta-analysis, the cumulative P-EPV regarding FN was 64% (95% CI 33% to 83%). GAS, based on data from the same publications and patients, yielded a positive EPV of 93% (95% CI 83% to 99%).ConclusionsThe results indicate that FN may play a role in PHC patients with an acute sore throat, but the association is much weaker compared with GAS.

show abstract

Real-time PCR investigation of the prevalence of Fusobacterium necrophorum in patients with pharyngitis in Japan

Cited by 11 publications

References 12 publications

Systematic review and meta-analysis of the accuracy of McIsaac and Centor score in patients presenting to secondary care with Pharyngitis

Systematic review and meta-analysis of the accuracy of McIsaac and Centor score in patients presenting to secondary care with Pharyngitis

The first case report: diagnosis and management of necrotizing fusobacterium lung abscess via BALF next-generation sequencing

Acute sore throat and Fusobacterium necrophorum in primary healthcare: a systematic review and meta-analysis

Contact Info

Product

Resources

About