Real-time PCR hTERT mRNA pattern in tumor core, edge, resection margin, and lymph nodes in laryngeal tumors: Relation to proliferative index and impact on prognosis

Eissa, Sanaa; Kenawy, Gamal; Moteleb, Fayda A.; El-Magkhzangy, Aly Nagy; Nassar, Mohamed

doi:10.1016/j.clinbiochem.2005.06.008

Cited by 9 publications

(3 citation statements)

References 20 publications

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“…Both methods showed that increasing hTERT expression is an early event in larynx carcinogenesis. This concurs with the results of Eissa et al (105), who with real-time PCR and fluorescence cytophotometry found lower hTERT levels in larynx tumor margin tissue than in the carcinomas.…”

Section: Cells Which May Be Responsible For Telomerase Activity Outsisupporting

confidence: 82%

Immunohistochemical determination of the appropriate anti-hTERT antibodies for in situ detection of telomerase activity in frozen sections of head and neck squamous cell carcinomas and tumor margin tissues

Fabricius

Kruse-Boitschenko²,

Khoury³

et al. 2009

Int J Oncol

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Abstract.In previous studies we demonstrated telomerase activity in frozen tissue from head and neck squamous cell carcinoma (HNSCC) and their tumor-free tumor margins. In the present study frozen sections from the same tissues were examined for in situ presence of hTERT. In preliminary investigations we established that the most suitable method of tissue preparation was fixation in acetone and methanol followed by steaming and visualization by APAAP. Most of the assays involved eleven anti-hTERT antibodies and were supplemented with the inclusion of antibodies Ki-67, antinucleolin and CD45. hTERT expression was investigated in the tissues of 61 patients with HNSCC and 37 patients without tumor. Semi-quantitative immunoreactive scores were correlated with telomerase activity. We examined the prognostic significance of hTERT expression with Kaplan-Meier curves and tested the immunological specificity of the antibodies by immunoabsorption with two hTERT peptides and a nucleolin peptide. Nuclear staining of satisfactory distribution and intensity was achieved in seven anti-hTERT antibodies both in the carcinomas and in the squamous epithelia of the tumor resection margins and in the control tissues. Proof of hTERT did not differ from telomerase activity. The telomerase activity demonstrated in tumor-free resection margins and in control tissues did, however, correlate with lymphocytic-monocytic infiltration (CD45 expression). This telomerase activity might be related to nuclear hTERT expression in the squamous epithelium, given that the hTERT score values in the connective tissue tended to be negative. The prognostic significance of hTERT expression demonstrated on paraffin sections from different tumor localizations was not confirmed for the frozen sections of patients with HNSCC. The hTERT specificity of the monoclonal NCL-L-hTERT, whose use as an antibody against hTERT has been questioned, was reexamined with immunohistochemical methods, but the intensity of its immunoabsorption with the nucleolin peptide did not exceed that observed in the other anti-hTERT antibodies.

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Section: Cells Which May Be Responsible For Telomerase Activity Outsisupporting

confidence: 82%

Immunohistochemical determination of the appropriate anti-hTERT antibodies for in situ detection of telomerase activity in frozen sections of head and neck squamous cell carcinomas and tumor margin tissues

Fabricius

Kruse-Boitschenko²,

Khoury³

et al. 2009

Int J Oncol

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“…El Samny et al 7 showed that hTERT levels in tumour edges significantly affected 2-year survival in 35 cases of laryngeal carcinoma. In a 2-year survival follow-up of 35 patients with laryngeal carcinoma, Eissa et al 17 found that tumour edge hTERT levels were significantly different between survivors and non-survivors. On the other hand, Koscielny et al 6 analysed telomerase activity in 80 cases of HNSCC, with follow-up beyond 2 years, and found no correlation between telomerase activity and survival.…”

Section: Discussionmentioning

confidence: 99%

Effects of Telomerase Activity on Carcinogenesis and Survival in Laryngeal Carcinoma

2008

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This study aimed to look at whether a correlation exists between telomerase activity and survival of laryngeal carcinoma patients. Telomerase activity was measured by telomerase repeat amplification protocol in 31 laryngeal carcinomas and adjacent normal tissues, and in 21 vocal cord polyps (controls). Follow-up was for at least 60 months. Telomerase activity in tissues adjacent to laryngeal carcinomas was significantly higher than in the carcinomas which was, in turn, significantly higher than in vocal cord polyps. There was no significant difference between telomerase activity in carcinomas or adjacent tissues and clinicopathological characteristics. Patients with high telomerase activity in carcinoma tissue had significantly shorter survival times than those with low activity, but no significance difference was observed between survival time and telomerase status in adjacent tissues. Linear regression showed significant association between telomerase activity levels in carcinoma tissues and survival time, but this was not observed in adjacent tissues. This study suggests that telomerase activation probably takes place before the cancer phenotype develops and has prognostic significance for survival of patients with laryngeal carcinoma.

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“…Similarly, local recurrence in patients treated by surgery alone may be due to microscopic cancer cells in the resection margins overlooked by conventional microscopy. Preliminary studies suggest that HNSCC transcriptional signatures could be used to detect invaded surgical margins to better select patients eligible for postoperative treatments 7, 8. To date, this potential field of application of GEP remains unexplored.…”

Section: Clinical Perspectives For Head and Neck Squamous Cell Carcinmentioning

confidence: 99%

Gene expression profiling in head and neck squamous cell carcinoma: Clinical perspectives

et al. 2010

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Real-time PCR hTERT mRNA pattern in tumor core, edge, resection margin, and lymph nodes in laryngeal tumors: Relation to proliferative index and impact on prognosis

Cited by 9 publications

References 20 publications

Immunohistochemical determination of the appropriate anti-hTERT antibodies for in situ detection of telomerase activity in frozen sections of head and neck squamous cell carcinomas and tumor margin tissues

Immunohistochemical determination of the appropriate anti-hTERT antibodies for in situ detection of telomerase activity in frozen sections of head and neck squamous cell carcinomas and tumor margin tissues

Effects of Telomerase Activity on Carcinogenesis and Survival in Laryngeal Carcinoma

Gene expression profiling in head and neck squamous cell carcinoma: Clinical perspectives

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