Real-time PCR for Quantification of Helicobacter felis in Mouse Stomach

Bégué, Rodolfo E.; Manning, Jennifer E.; Correa, Hernán

doi:10.1097/01.smj.0000247299.17826.a7

Cited by 2 publications

(1 citation statement)

References 5 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Four weeks after the challenge, mice were euthanized and the stomach was harvested to determine the presence of H. pylori organisms. Stomachs were homogenized (Tissue Tearor, Biospec Products, Bartlesville, OK), DNA was extracted (Dneasy Tissue Kit, Qiagen) and subjected to quantitative real‐time PCR (Bégué et al , 2006) using primers described previously by Roussel et al (2007) and targeted to the H. pylori SS1 16S rRNA gene (411–564 bp). Specimens were run in duplicates and positive and negative controls ( H. pylori ‐infected and ‐uninfected mice, respectively) were included.…”

Section: Methodsmentioning

confidence: 99%

Protective efficacy of recombinant urease B and aluminum hydroxide against Helicobacter pylori infection in a mouse model

Bégué

Sadowska-Krowicka

2010

FEMS Immunology &Amp; Medical Microbiology

Self Cite

View full text Add to dashboard Cite

Efforts are underway for the development of an effective vaccine against Helicobacter pylori infection. We prepared recombinant full length (568 aa) Helicobacter pylori urease B protein (rUreB) and tested it for immunogenicity and protection. BALB/c mice received either rUreB (40 μg) plus CpG (10 μg) intranasally, rUreB (50 μg) plus 3% aluminum hydroxide (50 μL) intramuscularly or rUreB (25 μg) plus Freund’s adjuvant (25 μL) subcutaneously, three times (week 0, 2 and 6). Intranasal rUreB plus CpG was neither immunogenic nor protective; intramuscular rUreB plus aluminum hydroxide was immunogenic and modestly protective; and subcutaneous rUreB plus Freund’s adjuvant was immunogenic and highly protective. The fact that protection was improved with Freund’s adjuvant indicates that recombinant urease B is a good antigen for a vaccine but that it needs a stronger adjuvant than aluminum hydroxide.

show abstract

Section: Methodsmentioning

confidence: 99%

Protective efficacy of recombinant urease B and aluminum hydroxide against Helicobacter pylori infection in a mouse model

Bégué

Sadowska-Krowicka

2010

FEMS Immunology &Amp; Medical Microbiology

Self Cite

View full text Add to dashboard Cite

show abstract

Life-threatening angioedema of the tongue: the detection of the RNA ofB henselaein the saliva of a male patient and his dog as well as of the DNA of threeBartonellaspecies in the blood of the patient

Lösch

Wank²

2014

BMJ Case Reports

View full text Add to dashboard Cite

Non-hereditary angioedema is a common disease with a prevalence between 5% and 19% and approximately half of the patients experience a swelling of the tongue. We report a case of a 49-year-old Caucasian man with a gross life-threatening angioedema of the tongue, whose attacks occurred every 4 weeks. The most frequent causes of angioedema were excluded. We detected DNA and RNA from Bartonella henselae in the blood and saliva of the patient and in the saliva of the patient's hunting dog. Treatment with azithromycin plus minocycline cleared the blood and saliva of RNA and DNA of Bartonella species, and the patient has been free from angioedema for 1 year. None of the therapy modalities used to treat the hereditary form or ACE or allergy-induced angioedema affect the detrimental course caused by Bartonella species. We therefore suggest that a molecular Bartonella test be included in the analysis of angioedema.

show abstract

Real-time PCR for Quantification of Helicobacter felis in Mouse Stomach

Cited by 2 publications

References 5 publications

Protective efficacy of recombinant urease B and aluminum hydroxide against Helicobacter pylori infection in a mouse model

Protective efficacy of recombinant urease B and aluminum hydroxide against Helicobacter pylori infection in a mouse model

Life-threatening angioedema of the tongue: the detection of the RNA ofB henselaein the saliva of a male patient and his dog as well as of the DNA of threeBartonellaspecies in the blood of the patient

Contact Info

Product

Resources

About