Real-Time PCR for Gene Expression Analysis

Yılmaz, Akın; Onen, Hacer Ilke; Alp, Ebru; Menevşe, Sevda

doi:10.5772/37356

Cited by 5 publications

(1 citation statement)

References 95 publications

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“…The reference gene should not be regulated or influenced by the experimental conditions (Yilmaz et al, 2012). The most commonly used reference genes considered to normalise data include; the Elongation factor 1 protein (AGAP003541), Ribosomal protein s7 (AGAP010592) and Ubiquitin (AGAP007927) primers (Table 5.3).…”

Section: Primer Designmentioning

confidence: 99%

Regulatory mechanisms involved in the control of CYP6M2 gene in insecticide resistant in Anopheles gambiae (Diptera: Culicidae)

Mohammed

2014

Journal of Biotechnology

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Cytochrome P450s, including CYP6M2 gene, are involved in the detoxification of permethrin. Some of these genes are regulated by CnCC / dKeap 1 and Spineless / Tango in Drosophila melanogaster. This mechanism is yet to be identified in Anopheles gambiae. Thus, we examine whether there is differential regulation of CYP6M2 gene between permethrin resistant Tiassalé and susceptible Kisumu strains of An. gambiae. Bioinformatics analysis was used to search for cis-acting elements within CYP6M2 (896 bp) region hypothesised to contain the promoter. Isolated and cloned CYP6M2 promoter reporter constructs were transfected into Anopheles gambiae Sua 5.1* cells to measure luciferase activity as a surrogate promoter activity. The WHO adult bioassay was used to expose adult females of the permethrin resistant Tiassalé and susceptible Kisumu strains of An. gambiae to discriminating doses of 0.75% permethrin. Uncharacterised strains from Auyo (Auyo-Nigeria) selected to 4% DDT and 0.1% Regulatory Mechanisms Involved in the Control of CYP6M2

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Section: Primer Designmentioning

confidence: 99%