Real-Time PCR for Diagnosis of Human Bocavirus Infections and Phylogenetic Analysis

Neske, Florian; Blessing, Kerstin; Tollmann, Franz; Schubert, Jörg; Rethwilm, Axel; Kreth, Hans Wolfgang; Weißbrich, Benedikt

doi:10.1128/jcm.00027-07

Cited by 91 publications

(97 citation statements)

References 24 publications

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“…Since then, the detection of HBoV in acute respiratory illness has been reported worldwide, with prevalence rates between 1.5 and 19% (13)(14)(15). It was subsequently detected in serum (16)(17), fecal (18)(19) and urine samples (20). HBoV detection was significantly higher in patients with symptoms of respiratory tract infections or gastroenteritis than in asymptomatic individuals (14,16,(21)(22)(23).…”

Section: Introductionmentioning

confidence: 99%

High prevelance of human parvovirus infection in patients with malignant tumors

Dong

Jiang

et al. 2012

Oncology Letters

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Abstract.It is well known that the immunity of patients with malignant tumors decreases significantly. An increased parvovirus B19 (B19) infection rate has been observed in immunocompromised hosts. However, only a small amount of literature regarding the risk of human parvovirus infection in patients with malignant tumors is available. To evaluate the correlation of human parvovirus infection with malignant tumors, 288 serum samples from patients with malignant tumors were screened for B19 DNA by nested-PCR. The serum samples, 156 of which were from known clinicopathological cancer patients, were subjected to analysis of the seropositive rate of human bocavirus (HBoV), hepatitis B virus (HBV) and transfusion transmitted virus (TTV) by PCR. A total of 800 normal population sera and 941 aspirate samples from children with respiratory tract infections were used as controls for the detection of B19 and HBoV, respectively. Pairwise comparison between cancerous serum and control samples, and the correlation between parvovirus infection and clinicopathological variables, including gender and cancer type, were evaluated using the χ2 test, Fisher's exact test or the t-test. P<0.05 was considered to indicate a statistically significant difference. The overall prevalence of B19 DNA in cancer patients was 50.69% (146/288), which was significantly higher than that of the healthy controls with 4.5% (36/800) (χ2 test, P<0.0001). Similar results were obtained for HBoV with a 39.74% (62/156) prevalence in cancer patients. However, the infection prevalence of HBV and TTV in the cancer patients was 5.13 (8/156) and 6.41% (10/156), respectively (P<0.0001), which was much less than that of B19 and HBoV. These results revealed that a high risk of B19 and HBoV infection occurred in cancer patients, and a potential correlation exists between parvovirus infection and occurrence of malignant tumors.

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Section: Introductionmentioning

confidence: 99%

High prevelance of human parvovirus infection in patients with malignant tumors

Dong

Jiang

et al. 2012

Oncology Letters

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“…In a large respiratory disease survey, diarrhea was reported in 16% of HBoV-positive patients (4). In another survey, HBoV was detected in 14 (45%) of 31 fecal samples from children with HBoV-positive respiratory samples (15). HBoV has been detected in 2 to 9% of fecal samples (n, 527 to 1,435) from children with gastroenteritis (1,12,19).…”

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confidence: 98%

Human Bocavirus in an Immunocompromised Child Presenting with Severe Diarrhea

et al. 2009

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Human bocavirus (HBoV) is frequently detected in young children with respiratory symptoms. However, the prevalence and pathogenicity of HBoV in immunocompromised patients are largely unknown. This report describes a case of life-threatening hypovolemic shock due to diarrhea associated with disseminated HBoV infection in an immunocompromised child. CASE REPORTA 9-year-old boy with erythropoietic protoporphyria was admitted to the University Medical Center Groningen, Groningen, The Netherlands. He had undergone orthotopic liver transplantation and allogeneic hematopoietic stem cell transplantation 9 and 4 months prior to admission, respectively. He presented with fever, nausea, vomiting, and diarrhea of 1-day duration. Clinical examination showed a moderately sick boy with a temperature of 39.5°C, tachycardia (pulse rate, 150 beats per min), normal blood pressure, and normal hydration status. Pulmonary examination detected a minor cough and minor rales upon auscultation. Examination of the abdomen revealed hyperperistalsis, no tenderness, and palpable edges of the liver and spleen. Laboratory results showed an elevated C-reactive protein level (51 mg liter Ϫ1 ; normal, Յ10 mg liter Ϫ1 ) and erythrocyte sedimentation rate (106 mm h Ϫ1 ; normal, Յ25 mm h Ϫ1 ) and a normal leukocyte count. The patient had severe therapy-induced lymphopenia (20 CD3 ϩ T cells l Ϫ1 ; normal range, 700 to 4,200 l Ϫ1 ). Liver chemistry test results were stable but elevated due to biliary complications after liver transplantation. Bacterial blood cultures and fecal cultures for Campylobacter, Salmonella, and Shigella spp. and Escherichia coli O157 were negative. PCR analyses of feces for noroviruses (genogroups I and II), adenovirus, parechovirus, enterovirus, and cytomegalovirus and antigen detection assays of feces for rotavirus and astrovirus were all negative. In contrast, feces results were positive for human bocavirus (HBoV) by PCR (threshold cycle [C T ] value in week 49 of 2007, 17) (Fig.

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“…106(1): 56-60, February 2011 Respiratory tract infections are caused mostly by viruses, including respiratory syncytial virus (RSV), influenza viruses A (FLU A) and B (FLU B), parainfluenza viruses (PIV), human adenovirus (ADV) and human rhinoviruses (RNV). Recently, with the advent of molecular techniques and the introduction of these methods into the clinical laboratory, new respiratory viruses, such as the human metapneumovirus (hMPV) and human bocavirus (hBoV), were identified in respiratory tract specimens collected from patients with clinical symptoms of respiratory diseases (Neske et al 2007). hBoV is the latest pathological parvovirus discovered in Sweden, isolated from pooled nasopharyngeal aspirate specimens (Allander et al 2007).…”

mentioning

confidence: 99%

Detection of human bocavirus and human metapneumovirus by real-time PCR from patients with respiratory symptoms in Southern Brazil

Pilger¹,

Cantarelli

Amantéa

et al. 2011

Mem. Inst. Oswaldo Cruz

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The introduction of newer molecular methods has led to the discovery of new respiratory viruses, such as human metapneumovirus (hMPV) and human bocavirus (hBoV), in respiratory tract specimens. We have studied the occurrence of hMPV and hBoV in the Porto Alegre (PA) metropolitan area, one of the southernmost cities of Brazil, evaluating children with suspected lower respiratory tract infection from May 2007-June 2008. A real-time polymerase chain reaction method was used for amplification and detection of hMPV and hBoV and to evaluate coinfections with respiratory syncytial virus (RSV), influenza A and B, parainfluenza 1, 2 and 3, human rhinovirus and human adenovirus. Of the 455 nasopharyngeal aspirates tested, hMPV was detected in 14.5% of samples and hBoV in 13.2%. A unique causative viral agent was identified in 46.2% samples and the coinfection rate was 43.7%. For hBoV, 98.3% of all positive samples were from patients with mixed infections. Similarly, 84.8% of all hMPV-positive results were also observed in mixed infections. Both hBoV and hMPV usually appeared with RSV. In summary, this is the first confirmation that hMPV and hBoV circulate in PA; this provides evidence of frequent involvement of both viruses in children with clinical signs of acute viral respiratory tract infection, although they mainly appeared as coinfection agents

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Real-Time PCR for Diagnosis of Human Bocavirus Infections and Phylogenetic Analysis

Cited by 91 publications

References 24 publications

High prevelance of human parvovirus infection in patients with malignant tumors

High prevelance of human parvovirus infection in patients with malignant tumors

Human Bocavirus in an Immunocompromised Child Presenting with Severe Diarrhea

Detection of human bocavirus and human metapneumovirus by real-time PCR from patients with respiratory symptoms in Southern Brazil

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