Real-time PCR detection of Listeria monocytogenes using an integrated microfluidics platform

Cady, Nathaniel C.; Stelick, Scott; Kunnavakkam, Madanagopal V.; Batt, Carl A.

doi:10.1016/j.snb.2004.10.022

Cited by 153 publications

(117 citation statements)

References 35 publications

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“…[12][13][14][15] There have been many reports of lSPE DNA extraction. [12][13][14][15][16][17][18][19][20][21][22][23][24][25][26][27] Previous reports describe DNA lSPE systems with integrated polymerase chain reaction 21,22 and electrophoretic separation. [23][24][25] Furthermore, DNA lSPE has been applied to forensics, where samples are typically very dilute.…”

Section: Introductionmentioning

confidence: 99%

Rapid microfluidic solid-phase extraction system for hyper-methylated DNA enrichment and epigenetic analysis

Sparreboom

Berg

et al. 2014

Biomicrofluidics

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Genetic sequence and hyper-methylation profile information from the promoter regions of tumor suppressor genes are important for cancer disease investigation. Since hyper-methylated DNA (hm-DNA) is typically present in ultra-low concentrations in biological samples, such as stool, urine, and saliva, sample enrichment and amplification is typically required before detection. We present a rapid microfluidic solid phase extraction (lSPE) system for the capture and elution of low concentrations of hm-DNA ( 1 ng ml À1), based on a protein-DNA capture surface, into small volumes using a passive microfluidic lab-on-a-chip platform. All assay steps have been qualitatively characterized using a real-time surface plasmon resonance (SPR) biosensor, and quantitatively characterized using fluorescence spectroscopy. The hm-DNA capture/elution process requires less than 5 min with an efficiency of 71% using a 25 ll elution volume and 92% efficiency using a 100 ll elution volume. V C 2014 AIP Publishing LLC.

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Section: Introductionmentioning

confidence: 99%

Rapid microfluidic solid-phase extraction system for hyper-methylated DNA enrichment and epigenetic analysis

Sparreboom

Berg

et al. 2014

Biomicrofluidics

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“…Cady et al [21] constructed the PCR amplification chamber using soft lithography techniques for polydimethylsiloxane (PDMS) and SU-8 photoresist tested for their ability to purify and [26]. Fig.…”

Section: Chamber Stationary Pcr Chipmentioning

confidence: 99%

Microfluidic PCR Chips

Chen¹,

Chen²,

Yuan³

et al. 2011

Nano BioMed ENG

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The microfluidic polymerase chain reaction (PCR) chips have undergone extensive development and nowadays have become an important domain of miniaturization technology application. Here, we review the advances of microfluidic PCR chips over the past years, from the first single chamber stationary PCR chip to the new SlipChip PCR. First, the three distinct types of microfluidic PCR chips are discussed, including chamber stationary PCR chips, flow-through PCR chips and convection PCR chips. Then we focus on droplet PCR chips and SlipChip PCR. Although they are at an early stage, they show the great potential for high-throughput PCR and robust chip. Finally, general discussions on integrated chips are given. The low cost, portable, high-throughout integrated PCR chips will certainly be further developed in spite of many challenges.

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“…From point of view of LOC material, two main requirements have to be fulfilled: (1) optical transparency necessary for introduction of the fluorescence excitation light and collection of the fluorescence light from an area of interest within the chip, and (2) low autofluorescence of LOC's material to minimize optical background signal. The chips can be whole made as multilayer construction of light-transparent materials -like glass, PDMS, SU-8, COC or other polymers, or only a top cover of the chip is made of these materials whereas body of the chip is made of silicon or ceramic [4][5][6][7][8][9][10][11]. It is also possible to make LOC from non-transparent materials like low-temperature co-fired ceramics (LTCC) but an integration of optical waveguides enabling introduction of the fluorescence inducing light into a detection chamber and covered with glass or PDMS is necessary [12].…”

Section: Detection Of Fluorescence In Locmentioning

confidence: 99%

“…In the detection channel, fluorescence light emitted by the fluorochrome is collected by the microscope objective with proper magnification, and guided to a photodetector by passing through filter(s) and dichroic mirror(s) to exclude the excitation light. Common detectors are cooled photomultiplier tubes (PMT) [5,6,11], semiconductor photodiodes [7,9,10], rather cooled charge coupled devices (CCD) as sensing matrix in video cameras [4] or lines in spectrophotometers [8].…”

Section: Detection Of Fluorescence In Locmentioning

confidence: 99%

Lab-on-a-chip fluorescence detection with image sensor and software-based image conditioning

Walczak¹

2011

Bulletin of the Polish Academy of Sciences: Technical Sciences

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Abstract. The fluorometric detection in lab-on-a-chip devices for life-sciences is one of the main detection methods. Well know from "large-scale" analytical chemistry methodologies and instrumentation has been applied in lab-on-a-chip solutions. In most cases, optical instrumentation for fluorescence induction and detection is based on configuration and components borrowed from large laboratory instruments. As a result, optical instrumentation surrounding lab-on-a-chip is bulky, expensive and dedicated for operation only inside laboratory. In this paper a discussion on fluorescence detection in lab-on-a-chip is carried out. A novel image sensor -based detection instrumentation co-working with a "clever" software is described. Instrumentation is dedicated for operation in portable and low-cost devices for different life-science applications. Examples of applications of the novel method and instrumentation co-working with various lab-on-a-chips are presented on the base of author's works.

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Real-time PCR detection of Listeria monocytogenes using an integrated microfluidics platform

Cited by 153 publications

References 35 publications

Rapid microfluidic solid-phase extraction system for hyper-methylated DNA enrichment and epigenetic analysis

Rapid microfluidic solid-phase extraction system for hyper-methylated DNA enrichment and epigenetic analysis

Microfluidic PCR Chips

Lab-on-a-chip fluorescence detection with image sensor and software-based image conditioning

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