2022
DOI: 10.3390/d14100839
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Real-Time PCR-Based Method for Sex Determination in Lake Sturgeon (Acipenser fulvescens)

Abstract: The characterization of demographic features in natural populations is important to understand recruitment dynamics, mating systems, and propensities for loss of genetic diversity. The accurate identification of sex can be difficult in sexually monomorphic species. We present a melt curve analysis based on qPCR assays for lake sturgeon (Acipenser fulvescens) sex identification and validation using the AllWSex2 marker and adult males and females (total N = 143) with an assigned field sex from two populations (B… Show more

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Cited by 3 publications
(1 citation statement)
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“…It is possible that separating recapture histories by sex would have led to different results; however, sex could not be accurately assigned. Since tissue samples were collected from each Lake Sturgeon, in the future it is anticipated that sex will be assigned using molecular techniques and applied to the upper St. Clair River subpopulation to provide a more precise estimate of population parameters (Kanefsky et al 2022). For these reasons, the breeding return time model was not suitable for our data, but its use should be reevaluated when additional sex and recapture information becomes available.…”
Section: Discussionmentioning
confidence: 99%
“…It is possible that separating recapture histories by sex would have led to different results; however, sex could not be accurately assigned. Since tissue samples were collected from each Lake Sturgeon, in the future it is anticipated that sex will be assigned using molecular techniques and applied to the upper St. Clair River subpopulation to provide a more precise estimate of population parameters (Kanefsky et al 2022). For these reasons, the breeding return time model was not suitable for our data, but its use should be reevaluated when additional sex and recapture information becomes available.…”
Section: Discussionmentioning
confidence: 99%