Real-Time PCR and its Applications in Human Papillomavirus Quantitation and Physical Status Identification

Draganov, P.; Kalvatchev, Z.; Papassavva, V.; Sapunarova, S.

doi:10.1080/13102818.2004.10817104

Cited by 5 publications

(5 citation statements)

References 14 publications

(15 reference statements)

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“…Compared to other studies, we con rmed 15 types of hr-HPV and the viral load of each type by using the HPV qRT-PCR Kit(Liferiver, Shanghai, China). This PCR-based HPV test has high sensitivity and speci city[ [28,29]. Although our study is a retrospective study with selection bias, it had a large sample size, initial HPV infection status, and consistent post-operative HPV infection status during the follow-up time.…”

Section: Discussionmentioning

confidence: 99%

The Prognostic Value of High-risk Human Papillomavirus Infection Status in Surgically Treated Stage IB1-IIA2 Cervical Squamous Cell Carcinoma

Zeng¹,

Zuo²,

Li³

et al. 2020

Preprint

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Background: High-risk HPV(hr-HPV) infection is important for the development of invasive cervical cancer. As a developing country with the largest population in the world, China has a great burden of cervical cancer. The cervical cancer screening strategies consisted of cytology and virology, which has been studied by HPV-typing test and HPV-DNA quantitative test since decades ago. The cervical cancer incidence rate has been declined due to the early treatment of precancerous lesions, but postoperative HPV infection is still an issue for cervical cancer patients. This study aims to investigate the association between HPV infection status and recurrence of early-stage cervical cancer through a novel PCR-based HPV test, which could do both HPV typing-test and DNA quantitative-test. Methods: Patients diagnosed with cervical cancer staged IB1- IIA2, who were treated by radical hysterectomy and lymphadenectomy at Cancer Hospital Chinese Academy of Medical Sciences(CAMS) between January 2014 and December 2016 were accrued. The clinicopathological factors, pre- and post-operative HPV infection status, and the prognosis were investigated. Cox regression was used to identify factors associated with LRFS, MFS and OS. Results: A total of 312 patients were enrolled in this study, who were treated by radical resection and accepted pre- and post-operative HPV tests, with a median follow-up time of 60 months(range 14~79 months). The 5-year LRFS rate, MFS rate, and OS rate were 97.8%, 98.4%, and 98.7%. The pre-operative HPV infection rate was 85.3%(266/312), 74 patients had a high level of HPV-DNA(>5x106 copy number/104 cells). Twenty-nine patients had a postoperative persistent high level of HPV-DNA(9.3%). Postoperative persistent high level of HPV-DNA within 12 months(p=0.013), postoperative persistence of HPV-16/18 within 24 months(p=0.004), and deep stromal invasion(>2/3)(p=0.007) were associated with a poor LRFS.Conclusion: Pre-operative HPV-16/18 infection and high level of HPV-DNA were not associated with local recurrence of cervical cancer. Most initial HPV-positive patients had HPV cleared within 24 months postoperatively. Postoperative HPV-16/18 persistence within 24 months, postoperative persistence of high HPV-DNA level within 24 months, and deep stromal invasion(>2/3) were independent risk factors for local recurrence of cervical cancer.

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Section: Discussionmentioning

confidence: 99%

The Prognostic Value of High-risk Human Papillomavirus Infection Status in Surgically Treated Stage IB1-IIA2 Cervical Squamous Cell Carcinoma

Zeng¹,

Zuo²,

Li³

et al. 2020

Preprint

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“…On the base of that gene (Genbank ID: AAO91203, 1, locus CBU_1708, coordinates 1639188-1639769, length 582 nt) and D-LUX TM Designer (http//www.invitrogen.com/lux) we have generated 20 LUX primer pairs, using the new technology for nucleotide marking of (Invitrogen Press Room Online; http://www.invitrogen. com) (6,7).…”

Section: Methodsmentioning

confidence: 98%

“…(18), molecular-biological methods (conventional PCR, real-time PCR) are used (6,7,12,13). Coxiella burnetii isolation must be performed only in the set of high bio-security laboratories, level 3.…”

Section: Introductionmentioning

confidence: 99%

Effective Lux (Light Upon eXtension) Primer System for Early and Rapid Detection ofCoxiella burnetii

Kunchev

Alexandrov

Kamarinchev

et al. 2007

Biotechnology & Biotechnological Equipment

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“…This method has been used for different viral pathogens like hepatitis B virus (12), papillomaviruses (11), etc. There are different modifications of the PCR technique -multiplex PCR, nested PCR, RT -PCR, RealTime PCR (5,13) Reverse transcriptase -polymerase chain reaction (RT-PCR) is one of the most sensitive and specific molecular techniques that can simultaneously detect, type and subtype influenza viruses A and B in specimens of patients with respiratory infections. (2,18,20,21) In some of these studies, agarose gel analysis was used for the detection of the amplified gene region.…”

Section: Introductionmentioning

confidence: 99%

Application of Molecular Biology Methods for Detection of Influenza Viruses in Bulgaria

Hadzhiolova

Pavlova

Kotseva

2005

Biotechnology & Biotechnological Equipment

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Influenza viruses A and B are world-wide major causative agents of human acute respiratory infections. Three subtypes of HA (H1 to H3) and two subtypes of NA (N1 and N2) are found among influenza A viruses that have caused epidemics and pandemics among humans. For the rapid isolation and identification of influenza viruses in clinical samples variety of methods are available but the use of nucleic acid amplification techniques like RT-PCR has made sensitive diagnosis of influenza virus infection feasible, with the possibility of type and subtype determination. During the season 2002/2003 the laboratory of Influenza and ARD detected 7 type A and 4 type B influenza viruses by RT-PCR in 15 simulated samples from the respiratory panel from SEQC. In the season 2003 /2004 -42 positive results for influenza viruses subtype А/Н3N2/ were obtained by RT-PCR using specific НA and NA primer pairs from 50 nasal and throat swabs from patients with influenza-like diseases. The RT-PCR provides a sensitive and specific method for detecting, typing and subtyping influenza viruses A and B in clinical samples and can be used in the routine diagnosis of the disease in Bulgaria together with a large complex of classical methods.

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Real-Time PCR and its Applications in Human Papillomavirus Quantitation and Physical Status Identification

Cited by 5 publications

References 14 publications

The Prognostic Value of High-risk Human Papillomavirus Infection Status in Surgically Treated Stage IB1-IIA2 Cervical Squamous Cell Carcinoma

The Prognostic Value of High-risk Human Papillomavirus Infection Status in Surgically Treated Stage IB1-IIA2 Cervical Squamous Cell Carcinoma

Effective Lux (Light Upon eXtension) Primer System for Early and Rapid Detection ofCoxiella burnetii

Application of Molecular Biology Methods for Detection of Influenza Viruses in Bulgaria

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Real-Time PCR and its Applications in Human Papillomavirus Quantitation and Physical Status Identification

Cited by 5 publications

References 14 publications

The Prognostic Value of High-risk Human Papillomavirus Infection Status in Surgically Treated Stage IB1-IIA2&nbsp;Cervical Squamous Cell Carcinoma

The Prognostic Value of High-risk Human Papillomavirus Infection Status in Surgically Treated Stage IB1-IIA2&nbsp;Cervical Squamous Cell Carcinoma

Effective Lux (Light Upon eXtension) Primer System for Early and Rapid Detection ofCoxiella burnetii

Application of Molecular Biology Methods for Detection of Influenza Viruses in Bulgaria

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The Prognostic Value of High-risk Human Papillomavirus Infection Status in Surgically Treated Stage IB1-IIA2 Cervical Squamous Cell Carcinoma

The Prognostic Value of High-risk Human Papillomavirus Infection Status in Surgically Treated Stage IB1-IIA2 Cervical Squamous Cell Carcinoma