Real-time observations of tooth demineralization in 3 dimensions using X-ray microtomography

Davis, Graham; Mills, David; Anderson, Paul

doi:10.1016/j.jdent.2017.11.010

Cited by 22 publications

(15 citation statements)

References 21 publications

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“…A comparative analysis of two techniques (microCT and LM) was performed. The field of tooth erosion research is changing rapidly with a new area of research into the 3D dynamic processes involved in tooth demineralization and remineralization (Davis et al, 2018). The number of publications rising sharply, which is undoubtedly related to the technological development in mineralized tissue research, leading to numerous advances in our understanding of physical and chemical properties of tooth structure (Johansson et al, 2012; Schlueter, Hara, Shellis, & Ganss, 2011).…”

Section: Discussionmentioning

confidence: 99%

“…A number of reviews of tooth erosion have been published over the past decade (Attin & Wegehaupt, 2014), but they have focused in clinical practice and there is in fact a knowledge gap regarding the comparison of methods available for quantitative tooth erosion analysis even if is demonstrated that microCT can quantify real‐time demineralization or demineralization (Davis, Mills, & Anderson, 2018). Therefore, the aim of this paper is to compare dentin and enamel erosion depth measurements using micro‐computed tomography and light microscopy.…”

Section: Introductionmentioning

confidence: 99%

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Measurement of erosion depth using microcomputed tomography and light microscopy

Roque‐Torres

Kwon

Oyoyo

et al. 2020

Microscopy Res & Technique

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Tooth-erosion is the surface loss of dental hard tissue mostly associated with an acid attack. The aim was to compare dentin and enamel erosion depth measurements using micro-computed tomography (microCT) and light microscopy (LM). Enamel/ dentin blocks were prepared from caries-free human molar-teeth (N = 12). Teeth were sectioned to a rectangular shape of 4 × 4 × 6 mm. Specimens were treated with water (NC) or 1.0% citric-acid solution (PC). After treatment, specimens were scanned with micro-computed tomography. On completion, specimens were sectioned and observed under a light-microscope. Lesion depth was observed with 10× magnification and images transferred to Simpleware software. Vertical distance from lesion surface to bottom was measured. Pearson correlation test was used to evaluate correlation and Wilcoxon Signed Rank test to evaluate differences in the twoanalysis methods. Mean enamel erosion depth was 0.63 and 38.38 μm (microCT) and 0.54 and 39.43 μm (LM) for NC and PC, respectively. Dentin erosion depth was 0.72 and 48.05 μm (microCT) and 0.56 and 49.92 μm (LM) for NC and PC, respectively. There was a significant correlation between the two-analysis methods (r = 0.998; p < .001). No statistically significant difference in results were obtained when microCT and LM were compared (p = .584). This results obtained from the current study suggested that erosion depth measurements made using microCT and LM yielded comparable results. The microCT method is preferred if the conservation of specimens is desired.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Measurement of erosion depth using microcomputed tomography and light microscopy

Roque‐Torres

Kwon

Oyoyo

et al. 2020

Microscopy Res & Technique

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“…As previously mentioned, x-ray µCT has been broadly applied for a great number of biological structures especially hard tissue samples such as bones and teeth (Neues and Epple, 2008;Schulze et al, 2010;Zou et al, 2011;Davis et al, 2018). Although still not deeply explored, microtomographic analyses of soft tissues, including nervous tissue, can reveal the functional mechanisms of 3D cellular and subcellular structures based on its spatial organization and distribution (Figure 2).…”

Section: High-resolution Microtomography For the Study Of The Nervous Systemmentioning

confidence: 99%

“…In biology, computerized x-ray µCT has provided 3D images of the architecture of biological samples from many species of organisms including mice (Johnson et al, 2006;Fonseca et al, 2018), humans (Bonse et al, 1994;Salome et al, 1999;Mizutani et al, 2010), and insects (Metscher, 2009;van de Kamp et al, 2011;Smith et al, 2016). It has become a common method in studies associated with osteo and dental microstructures (Neues and Epple, 2008;Zou et al, 2011;Davis et al, 2018). Recently, microtomographic studies of soft tissues, which account for a major proportion of biological tissues, have shed light on the structural mechanism of biological functions (Happel et al, 2010;Walker et al, 2014).…”

Section: Introductionmentioning

confidence: 99%

Illuminating the Brain With X-Rays: Contributions and Future Perspectives of High-Resolution Microtomography to Neuroscience

et al. 2021

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The assessment of three-dimensional (3D) brain cytoarchitecture at a cellular resolution remains a great challenge in the field of neuroscience and constant development of imaging techniques has become crucial, particularly when it comes to offering direct and clear obtention of data from macro to nano scales. Magnetic resonance imaging (MRI) and electron or optical microscopy, although valuable, still face some issues such as the lack of contrast and extensive sample preparation protocols. In this context, x-ray microtomography (μCT) has become a promising non-destructive tool for imaging a broad range of samples, from dense materials to soft biological specimens. It is a new supplemental method to be explored for deciphering the cytoarchitecture and connectivity of the brain. This review aims to bring together published works using x-ray μCT in neurobiology in order to discuss the achievements made so far and the future of this technique for neuroscience.

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“…Dentistry and maxillofacial surgery (MFS) represent two sectors that affect the biomedical engineering context and in which there was an extensive use of the micro-CT due to the necessity to acquire detailed information of small and complex objects, mineralized structures [17][18][19], and with different densities. The market is characterized by innovative materials and solutions that require advanced technology in routine-based activity of dental labs and clinics as the micro-CT scanning [20], whose capabilities turn out to be indispensable [21][22][23].…”

Section: Introductionmentioning

confidence: 99%

Ten Years of Micro-CT in Dentistry and Maxillofacial Surgery: A Literature Overview

Campioni¹,

Pecci

Bedini

2020

Applied Sciences

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Micro-computed tomography (micro-CT) is a consolidated imaging technology allowing non-destructive three-dimensional (3D) qualitative and quantitative analysis by the observation of microstructures with high resolution. This paper aims at delivering a structured overview of literature about studies performed using micro-CT in dentistry and maxillofacial surgery (MFS) by analyzing the entire set of articles to portray the state of the art of the last ten years of scientific publications on the topic. It draws the scenario focusing on biomaterials, in vitro and in/ex vivo applications, bone structure analysis, and tissue engineering. It confirms the relevance of the micro-CT analysis for traditional research applications and mainly in dentistry with respect to MFS. Possible developments are discussed in relation to the use of the micro-CT combined with other, traditional, and not, techniques and technologies, as the elaboration of 3D models based on micro-CT images and emerging numerical methods. Micro-CT results contribute effectively with whose ones obtained from other techniques in an integrated multimethod approach and for multidisciplinary studies, opening new possibilities and potential opportunities for the next decades of developments.

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Real-time observations of tooth demineralization in 3 dimensions using X-ray microtomography

Abstract: Observation of the dynamics of demineralization and remineralization will aid in the development of therapies to treat and prevent dental caries.

Cited by 22 publications

References 21 publications

Measurement of erosion depth using microcomputed tomography and light microscopy

Measurement of erosion depth using microcomputed tomography and light microscopy

Illuminating the Brain With X-Rays: Contributions and Future Perspectives of High-Resolution Microtomography to Neuroscience

Ten Years of Micro-CT in Dentistry and Maxillofacial Surgery: A Literature Overview

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