Real‐Time Observation of Protein Aggregation at Liquid‐Liquid Interfaces in a Microfluidic Device
Dominik Zürcher,
Klaus Wuchner,
Paolo Arosio
Abstract:A droplet microfluidic device to capture in real‐time protein aggregation at liquid‐liquid interfaces is described. In contrast to conventional methods, typically characterized by a lag time between the application of interfacial stress and the measurement of protein aggregation, here protein adsorption, the formation of a viscoelastic protein layer, aggregation, and shedding of protein particles into solution is simultaneously monitored. The device is applied to analyze the stability of antibody formulations … Show more
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