Real Time Observation of Chemical Reactions of Individual Metal Nanoparticles with High-Throughput Single Molecule Spectral Microscopy

Cheng, Jing; Liu, Yang; Cheng, Xiaodong; He, Yan; Yeung, Edward S.

doi:10.1021/ac101933y

Cited by 49 publications

(52 citation statements)

References 44 publications

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“…The effective 'detection volume' of every nanoprobe is, thus, enlarged, making it possible to accumulate or react with more sulphides locally to achieve a lower detection limit. The imaging method utilized in the present study, which was previously developed in our laboratory, is a high-throughput single-particle dark-field spectral imaging technique 45 , in which a transmission grating beam splitter (TGBS) is placed in the light collection path ( Supplementary Fig. S1).…”

Section: Resultsmentioning

confidence: 99%

Highly sensitive sulphide mapping in live cells by kinetic spectral analysis of single Au-Ag core-shell nanoparticles

et al. 2013

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Hydrogen sulphide (H 2 S) is a gaseous signalling agent that has important regulatory roles in many biological systems but remains difficult to measure in living biological specimens. Here we introduce a new method for highly sensitive sulphide mapping in live cells via singleparticle plasmonic spectral imaging that uses Au-Ag core-shell nanoparticles as probes. This strategy is based on Ag 2 S formation-induced spectral shifts of the nanoprobes, which is not only highly selective towards sulphide but also shows a linear logarithmic dependence on sulphide concentrations from 0.01 nM to 10 mM. A theoretical model was established that successfully explained the experimental observations, suggesting that the local sulphide concentration as well as its oscillations can be determined indirectly from kinetic measurements of the spectral shifts of the nanoprobes. We demonstrated for the first time the realtime mapping of local variations of sulphide levels in live cells with nM sensitivity.

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Section: Resultsmentioning

confidence: 99%

Highly sensitive sulphide mapping in live cells by kinetic spectral analysis of single Au-Ag core-shell nanoparticles

et al. 2013

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“…The setup is similar to that described by Yeung et al[12, 13] and consists of a conventional slit imaging spectrometer with two important differences. First, a grating is selected that has a relatively coarse line pitch.…”

Section: Methodsmentioning

confidence: 99%

Dual-order snapshot spectral imaging of plasmonic nanoparticles

et al. 2011

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The development of truly scalable, multiplexed optical microarrays requires a detection platform capable of simultaneous detection of multiple independent signals in real-time. We present a technique we term dual order snapshot spectroscopic imaging (DOSSI) and demonstrate that it can be effectively used to collect spectrally-resolved images of a full field of view of sparsely located spots in real-time. Resonant peaks of plasmonic gold nanoparticles were tracked as a function of their surrounding refractive index. Measurement uncertainty analysis indicated that the spectral resolution of DOSSI in the described configuration is approximately 0.95 nm. Further, real-time measurements by DOSSI allowed discrimination between optically identical nanoparticles that were functionalized with two homologous small molecule ligands that bound to the same protein, albeit with different affinity, based purely on their different molecular interaction kinetics– a feat not possible with slower raster-type hyperspectral imaging systems, or other darkfield optical detection systems that solely rely on end-point measurements. Kinetic measurements of plasmon bands by DOSSI can be performed with a relatively simple optical system, thereby opening up the possibility of developing low-cost detectors for arrayed plasmonic diagnostics.

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“…Another example, where direct nanoplasmonic sensing was applied, is a study of the oxidation kinetics of individual Au nanorods by H 2 O 2 in the presence of Br -by Cheng et al [77] . In their work they successfully identifi ed heterogeneous oxidation reaction pathways and intermediate states unobservable in ensemble measurements.…”

Section: Corrosionmentioning

confidence: 99%

Nanoplasmonic sensing for nanomaterials science

Larsson¹,

Syrenova

Langhammer

2012

Nanophotonics

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Nanoplasmonic sensing has over the last two decades emerged as and diversifi ed into a very promising experimental platform technology for studies of biomolecular interactions and for biomolecule detection (biosensors). Inspired by this success, in more recent years, nanoplasmonic sensing strategies have been adapted and tailored successfully for probing functional nanomaterials and catalysts in situ and in real time. An increasing number of these studies focus on using the localized surface plasmon resonance (LSPR) as an experimental tool to study a process of interest in a nanomaterial, with a materials science focus. The key assets of nanoplasmonic sensing in this area are its remote readout, non-invasive nature, single particle experiment capability, ease of use and, maybe most importantly, unmatched fl exibility in terms of compatibility with all material types (particles and thin/thick layers, conductive or insulating) are identifi ed. In a direct nanoplasmonic sensing experiment the plasmonic nanoparticles are active and simultaneously constitute the sensor and the studied nano-entity. In an indirect nanoplasmonic sensing experiment the plasmonic nanoparticles are inert and adjacent to the material of interest to probe a process occurring in/on this material. In this review we defi ne and discuss these two generic experimental strategies and summarize the growing applications of nanoplasmonic sensors as experimental tools to address materials science-related questions.

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Real Time Observation of Chemical Reactions of Individual Metal Nanoparticles with High-Throughput Single Molecule Spectral Microscopy

Cited by 49 publications

References 44 publications

Highly sensitive sulphide mapping in live cells by kinetic spectral analysis of single Au-Ag core-shell nanoparticles

Highly sensitive sulphide mapping in live cells by kinetic spectral analysis of single Au-Ag core-shell nanoparticles

Dual-order snapshot spectral imaging of plasmonic nanoparticles

Nanoplasmonic sensing for nanomaterials science

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