Real-Time Observation for the Enzymatic Reaction of Phospholipid Membrane:  Application of the Time-Resolved Quasi-Elastic Laser Scattering Method

Morisaku, Toshinori; Yui, Hiroharu; Iwazumi, Masanori; Ikezoe, Yasuhiro; Fujinami, Masanori; Sawada, Tsuguo

doi:10.1021/ac030320b

Cited by 16 publications

(11 citation statements)

References 35 publications

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“…40 Most experiments were executed at an equilibrium state, while we have succeeded in observing the dynamic behavior of molecules at a liquid/liquid interface. As reported in our previous papers, some novel dynamic behavior of molecules or micelles have been found: for instance, phase transfer catalysis systems across a nitrobenzene/water interface, 41 anomalous interfacial tension change by AOT micro-emulsion collapses at a heptane/water interface, 42 and enzymatic reaction of phospholipid membranes at a tetradecane/water interface, 43 .…”

Section: Methodsmentioning

confidence: 53%

Chemical Oscillation with Periodic Adsorption and Desorption of Surfactant Ions at a Water/Nitrobenzene Interface

et al. 2004

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An interface formed by two immiscible liquids is a specific area where chemical composition and physical properties steeply change on the nano-meter scale.1 Some characteristic phenomena are found: for example, adsorption and desorption of molecules, molecular recognition, 2,3 solvent extraction. 4 Chemical oscillations at a liquid/liquid interface have also been drawing attention from researchers in terms of not only the nonlinear chemistry but also for studies of biomimetic system. Since Dupeyrat and Nakache 5 first reported the electrochemical potential oscillation at the water/nitrobenzene interface in the 1970's, many kinds of chemical oscillation systems, not only water/oil two-phase systems 5-11 but also water/oil/water threephase systems 12-28 or gas/liquid interfaces, [29][30][31][32] have been developed and extensively investigated.Recently, we found a new-type interfacial oscillatory phenomenon with periodic adsorption and desorption of sodium alkyl sulfate at a water/nitrobenzene interface. [23][24][25][26] As previously reported in other interfacial oscillations, our system also shows an oscillation of electrical potential between the aqueous phase and the oil. The electrical potential is generated by unbalanced distribution of charged elements, which is not a simple phenomenon because such a situation is caused by adsorption of ions at the liquid/liquid interface, differences of concentration of the ions between the two liquid phases, and differences of ion mobility across the interface. As a result, understanding the chemical oscillation only from an electrical potential measurement is quite difficult. That is why, while most experiments showed a periodic oscillation pattern of electrical potential as a common property, the mechanisms proposed were not always similar. Agriculture and Technology, Japan Chemical oscillations with periodic adsorption and desorption of surfactant ions, alkyl sulfate ions, at a water/nitrobenzene interface have been investigated. The interfacial tension was measured with a quasi elastic laser scattering (QELS) method and the interfacial electrical potential was obtained. We found that this oscillation consists of a series of abrupt adsorptions of ions, followed by a gradual desorption. In addition, we observed that each abrupt adsorption was always accompanied by a small waving motion of the liquid interface. From the analysis of the video images of the liquid interface or bulk phase, we could conclude that each abrupt adsorption is caused by nonlinear amplification of mass transfer of ions from the bulk phase to the liquid interface by a Marangoni convection, which was generated due to local adsorption of the surfactant ions at the liquid interface that resulted in the heterogeneity of the interfacial tension. In the present paper, we describe the mechanism of the chemical oscillation in terms of the hydrodynamic effect on the ion adsorption processes, and we also show the interfacial chemical reaction with ion exchange during the ion desorption process.

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Section: Methodsmentioning

confidence: 53%

Chemical Oscillation with Periodic Adsorption and Desorption of Surfactant Ions at a Water/Nitrobenzene Interface

et al. 2004

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“…[33][34][35][36][37][38][39][40][41][42][43] Briefly, the water/oil interface was irradiated with an Nd-YAG laser (JUNO 100, 96 mW, Showa Optronics Co., Japan) at 532 nm. The laser beam was split with a 95/5 beam splitter, and the 5% segment was introduced to an acousto-optic modulator (1205C-2, Isomet, Springfield, VA) and then to the interface as a reference beam.…”

Section: Apparatusmentioning

confidence: 99%

“…Kovalchuk et al [21][22][23][24][25][26][27][28][29][30][31][32] showed that the dynamics at an air/water or a water/ organic interface affect the heterogeneity of the interfacial tension caused by the addition of an alcoholic surfactant through a capillary. Using time-resolved quasi-elastic laser scattering (QELS) measurements, [33][34][35][36][37][38][39][40][41][42][43] Ikezoe et al [33][34][35] found that surfactant molecules are rapidly adsorbed and gradually desorbed when an aqueous surfactant solution is introduced to the interface between aqueous and organic phases with a capillary.…”

Section: Introductionmentioning

confidence: 99%

Effects of Surfactants and Electrolytes on Chemical Oscillation at a Water/Nitrobenzene Interface Investigated by Quasi-elastic Laser Scattering Method

Toyota¹,

Uchiyama²,

Kimura³

et al. 2013

ANAL. SCI.

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We used a time-resolved interfacial tension measurement method with quasi-elastic laser scattering to investigate the effects of electrolytes and various surfactants on the nonlinear dynamics of the chemical oscillation that occurred at a water/nitrobenzene interface when a surfactant was added to the interface through a capillary. For both cationic and anionic surfactants, an electrolyte in the water phase was required for slow desorption of the surfactant from the interface. In the absence of an electrolyte, repulsion between the polar head groups of the ionic surfactants hindered adsorption of the surfactant molecules at the interface, resulting in their rapid desorption. In contrast, the presence of an electrolyte induced adsorption of the surfactant ions by screening their charged polar heads. Zwitterionic and nonionic surfactants were also examined and we deduced that the salting out effect of the surfactant produced by the presence of an electrolyte results in strongly attractive interactions between the surfactant molecules and the water/nitrobenzene interface.

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“…4 It is known that there is a ripplon wave (physical transverse wave) at the interfaces caused by their interfacial tensions without external perturbation. 5 When an external force works on the interfaces artificially, they are deformed. Of course, a mechanical force disturbs the interfaces.…”

Section: Introductionmentioning

confidence: 99%

Microscopic Measurements of Deformation of Liquid Surfaces Induced by Localized Direct Current Electric Field

2008

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This study presents a new microscopic method for the measurements of the deformation of liquid surfaces induced by a localized direct current (dc) electric field in a noncontact manner. Since the dielectric constant of aqueous and organic liquids is larger than that of air, the liquids tend to occupy a space with a stronger electric field. The horizontal level of transparent surfaces was measured with a constructed microscopic system that possessed a resolution of about 2 μm. When a rod electrode (2.0 mm in radius) was brought near to a liquid surface vertically within 150 -200 μm and a dc voltage (50 -75 V) was applied, the surface just under the electrode rose by 4 -19 μm. The deformation of the liquid surfaces was quantitatively analyzed by using a dielectric force, surface tension, and hydrostatic pressure.

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Real-Time Observation for the Enzymatic Reaction of Phospholipid Membrane: Application of the Time-Resolved Quasi-Elastic Laser Scattering Method

Cited by 16 publications

References 35 publications

Chemical Oscillation with Periodic Adsorption and Desorption of Surfactant Ions at a Water/Nitrobenzene Interface

Chemical Oscillation with Periodic Adsorption and Desorption of Surfactant Ions at a Water/Nitrobenzene Interface

Effects of Surfactants and Electrolytes on Chemical Oscillation at a Water/Nitrobenzene Interface Investigated by Quasi-elastic Laser Scattering Method

Microscopic Measurements of Deformation of Liquid Surfaces Induced by Localized Direct Current Electric Field

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