Real‐time monitoring of trans‐epithelial electrical resistance in cultured intestinal epithelial cells: <scp>the</scp> barrier protection of water‐soluble dietary fiber

Majima, Atsushi; Handa, Osamu; Naito, Yuji; Suyama, Yosuke; Onozawa, Yuriko; Higashimura, Yasuki; Mizushima, Katsura; Morita, Mayuko; Uehara, Yukiko; Horie, Hideki; Iida, Takuya; Fukui, Akifumu; Dohi, Osamu; Okayama, Tetsuya; Yoshida, Naohisa; Kamada, Kazuhiro; Katada, Kazuhiro; Uchiyama, Kazuhiko; Ishikawa, Takeshi; Takagi, Tomohisa; Konishi, Hideyuki; Yasukawa, Zenta; Tokunaga, Makoto; Ōkubo, Tsutomu; Itoh, Yoshito

doi:10.1111/1751-2980.12456

Cited by 13 publications

(7 citation statements)

References 23 publications

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“…Before being fermented by microbes in the colon, dietary fibers can have a substantial impact on the intestine via modulation of intestinal barrier function and immune responses (Figure 1). GOS, inulins, pectins, and β‐galactomannan have been shown to support a functional intestinal epithelial barrier by modulation of tight junction protein assembly, goblet cell activation and function, regulation of epithelial cell growth and glycocalyx maturation 36–41 . In addition, in vitro studies suggest that fibers including inulin, GOS, FOS, and arabinoxylan hydrolysates can modulate epithelial cell, macrophage and dendritic cell cytokine and chemokine secretion, in part mediated by activation of peroxisome proliferator‐activated receptor gamma (PPARγ) 42–46 .…”

Section: Fiber Effects On the Immune Systemmentioning

confidence: 99%

“…GOS, inulins, pectins, and β-galactomannan have been shown to support a functional intestinal epithelial barrier by modulation of tight junction protein assembly, goblet cell activation and function, regulation of epithelial cell growth and glycocalyx maturation. [36][37][38][39][40][41] In addition, in vitro studies suggest that fibers including inulin, GOS, FOS, and arabinoxylan hydrolysates can modulate epithelial cell, macrophage and dendritic cell cytokine and chemokine secretion, in part mediated by activation of peroxisome proliferator-activated receptor gamma (PPARγ). [42][43][44][45][46] An additional mechanism for direct fiber effects on immune cells is their activation of pattern recognition receptors (PRRs) such as C-type lectin receptors (CLRs, e.g., β-glucans), galectins, or Toll-like receptors (mainly TLR-2 and TLR-4, e.g., GOS and FOS) on epithelial cells and cells of the innate immune system.…”

Section: Fib Er Effec Ts On the Immune S Ys Temmentioning

confidence: 99%

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Role of dietary fiber in promoting immune health—An EAACI position paper

Venter

Meyer

Greenhawt

et al. 2022

Allergy

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Recent decades have seen a rapid increase in chronic inflammatory disorders due to inappropriate or misdirected immune responses accompanied by insufficient development of immune regulatory networks. It is generally accepted that changes in environment, lifestyle, and dietary factors may play a role in the miseducation or deficient training of the immune system. 1-3 A shift away from traditional diets rich in plant-based foods to highly processed foods is thought to be particularly important for negatively affecting microbiome diversity and composition, species-specific characteristics, microbial metabolism, and immunological tolerance. 4,5 While we acknowledge that a range of nutritional factors may play a role in influencing immune function and immune regulation, in this review we will focus specifically on one dietary component-fiber.Dietary fiber is a complex dietary component, including carbohydrate polymers and oligomers, which makes up the non-digestible components of food. 6,7 All dietary fibers resist digestion in the small bowel and pass into the large bowel intact but differ in their physiochemical characteristics (e.g., solubility, viscosity, and fermentability), which determine their functionality in the gut and to what degree they are accessible by microbes. Most soluble fibers can be fermented by the gut microbiota, partially or completely, dependent on their chemical structure. Dietary fibers can be defined on the basis of their chemical compounds, on the basis of their functional compounds, or both. Slight differences in definitions of dietary fibers exist due to the wide range of non-digestible fibers that occur in nature. The European Food Safety Authority (EFSA) defines dietary fiber as "non-digestible carbohydrates plus lignin." 8 These include non-starch polysaccharides (NSP) cellulose, hemicelluloses, pectins, hydrocolloids (i.e., gums, mucilages, and β-glucans), resistant oligosaccharides, resistant starch (consisting of physically enclosed starch, some types of raw starch granules, retrograded amylose, chemically and/or physically modified starches), and lignin associated with the dietary fiber polysaccharides (Table 1).Prebiotics are often equated with dietary fibers, but only a subset of dietary fibers qualifies as prebiotics. Not all fibers are equally fermentable by the gut microbiota (Table 1), with considerable inter-individual variation in the potential in vivo fermentability of dietary fiber. [9][10][11] The term "prebiotic" was first defined by Gibson and Roberfroid over 25 years ago as "a non-digestible food ingredient that beneficially affects the host by selectively stimulating the growth and/or activity of one or a limited number of bacteria in the colon, and thus improving host health." 12 This definition has evolved to a more simplified version-"a substrate that is selectively utilized by host microorganisms conferring a health benefit." 13 The most common prebiotic fermentable fibers that have been studied for immune health benefits to date include inulin, fructo-oligosacc...

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Section: Fiber Effects On the Immune Systemmentioning

confidence: 99%

Section: Fib Er Effec Ts On the Immune S Ys Temmentioning

confidence: 99%

Role of dietary fiber in promoting immune health—An EAACI position paper

Venter

Meyer

Greenhawt

et al. 2022

Allergy

View full text Add to dashboard Cite

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“…Tight junctions act as a barrier to paracellular diffusion and TEER reflects the tightness of junctions between epithelial cells. ( 17 , 18 ) Monolayer cells were gently rinsed with Hank’s balanced salt solution (HBSS) containing 137 mM NaCl, 5.36 mM KCl, 1.26 mM CaCl 2 , 0.55 mM MgSO 4 , 0.44 mM KH 2 PO 4 , 0.34 mM Na 2 HPO 4 , 2.92 mM NaH 2 PO 4 and incubated for 30 min in an incubator. The formation of the cell monolayer was evaluated by measuring TEER values using Millicell-ERS (Millipore, Eschborn, Germany).…”

Section: Methodsmentioning

confidence: 99%

Enzymatically synthesized glycogen inhibited degranulation and inflammatory responses through stimulation of intestine

Yoshioka

Inoue

Yoshioka

et al. 2020

J. Clin. Biochem. Nutr.

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The patients of type I allergic diseases were increased in the developed countries. Recently, many studies have focused on food factors with anti-allergic activities. Enzymatically synthesized glycogen, a polysaccharide with a multi-branched α-1,4 and α-1,6 linkages, is a commercially available product from natural plant starch, and has immunostimulation activity. However, effect of enzymatically synthesized glycogen on the anti-allergic activity was unclear yet. In this study, we investigated that enzymatically synthesized glycogen inhibited allergic and inflammatory responses using a co-culture system consisting of Caco-2 and RBL-2H3 cells. Enzymatically synthesized glycogen inhibited antigen-induced β-hexosaminidase release and production of TNF-α and IL-6 in RBL-2H3 cells in the co-culture system. Furthermore, enzymatically synthesized glycogen inhibited antigen-induced phosphorylation of tyrosine kinases, phospholipase C γ1/2, mitogen-activated protein kinases and Akt. Anti-allergic and anti-inflammatory activities of enzymatically synthesized glycogen were indirect action through stimulating Caco-2 cells, but not by the direct interaction with RBL-2H3 cells, because enzymatically synthesized glycogen did not permeate Caco-2 cells. These findings suggest that enzymatically synthesized glycogen is an effective food ingredient for prevention of type I allergy through stimulating the intestinal cells.

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“…Via PRRs, different fiber ligands support a functional intestinal epithelial barrier by modulation of tight junction protein assembly, goblet cell function, regulation of epithelial cell growth and glycocalyx maturation. 22,23 Pectins, for example, have been shown to strengthen the mucus layer by activating goblet cells and by forming hydrogen bonds with mucins. 24 Dietary fibers directly modulate chemokine and cytokine production by intestinal epithelial cells, dendritic cells and macrophages, in part mediated by activation of PPARγ.…”

Section: Fiber Effects On the Immune Systemmentioning

confidence: 99%

Role of Dietary Fiber in Promoting Immune Health -- An EAACI Position Paper

Venter

Greenhawt

Pali‐Schöll

et al. 2021

Preprint

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Microbial metabolism of specific dietary components, such as fiber, contribute to the sophisticated inter-kingdom dialogue in the gut that maintains a stable environment with important beneficial physiological, metabolic, and immunological effects on the host. Historical changes in fiber intake may be contributing to the increase of allergic and hypersensitivity disorders as fiber-derived metabolites are evolutionarily hardwired into the molecular circuitry governing immune cell decision making processes. In this review, we highlight the importance of fiber as a dietary ingredient, its effects on the microbiome, its effects on immune regulation, and potential mechanisms for dietary fibers in the prevention and management of allergic diseases. In addition, we review the human studies examining fiber or prebiotic interventions on asthma and respiratory outcomes, allergic rhinitis, atopic dermatitis, and overall risk of atopic disorders. While exposures, interventions and outcomes were too heterogeneous for meta-analysis, there is significant potential for using fiber in targeted manipulations of the gut microbiome and its metabolic functions in promoting immune health.

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Real‐time monitoring of trans‐epithelial electrical resistance in cultured intestinal epithelial cells: the barrier protection of water‐soluble dietary fiber

Cited by 13 publications

References 23 publications

Role of dietary fiber in promoting immune health—An EAACI position paper

Role of dietary fiber in promoting immune health—An EAACI position paper

Enzymatically synthesized glycogen inhibited degranulation and inflammatory responses through stimulation of intestine

Role of Dietary Fiber in Promoting Immune Health -- An EAACI Position Paper

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