Real-time monitoring of the pH microenvironment at the interface of multispecies biofilm and dental composites

Nguyễn, Anh Tuấn; Goswami, Subir; Ferracane, Jack L.; Koley, Dipankar

doi:10.1016/j.aca.2022.339589

Cited by 6 publications

(5 citation statements)

References 23 publications

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“…Although the DDPA successively eliminated some sensor noise on the conventional sensors, the discrepancy became considerable (average δ = 6.9 mg/L) after 30 days (Figure b), indicating that the DDPA failed to further enhance the conventional S-ISE sensor reading accuracy since the sensor lost its functionality due to biofilm penetration. , By contrast, the superior anti-fouling property of the PTFEMA- r -SBMA-loaded S-ISE sensor prolonged the sensor’s lifespan and consequently extended the effective time length of the DDPA to 50 days (Figure c). Therefore, we applied the DDPA in PTFEMA- r -SBMA-loaded S-ISE sensor readings for 55 days to examine the long-term rectification capability of the integrated sensor material advancement and sensor data processing.…”

Section: Resultsmentioning

confidence: 99%

Electrospraying Zwitterionic Copolymers as an Effective Biofouling Control for Accurate and Continuous Monitoring of Wastewater Dynamics in a Real-Time and Long-Term Manner

Huang

Qian

Wang

et al. 2022

Environ. Sci. Technol.

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Long-term continuous monitoring (LTCM) of water quality can provide high-fidelity datasets essential for executing swift control and enhancing system efficiency. One roadblock for LTCM using solid-state ion-selective electrode (S-ISE) sensors is biofouling on the sensor surface, which perturbs analyte mass transfer and deteriorates the sensor reading accuracy. This study advanced the anti-biofouling property of S-ISE sensors through precisely coating a self-assembled channel-type zwitterionic copolymer poly(trifluoroethyl methacrylate-random-sulfobetaine methacrylate) (PTFEMA-r-SBMA) on the sensor surface using electrospray. The PTFEMA-r-SBMA membrane exhibits exceptional permeability and selectivity to primary ions in water solutions. NH 4 + S-ISE sensors with this anti-fouling zwitterionic layer were examined in real wastewater for 55 days consecutively, exhibiting sensitivity close to the theoretical value (59.18 mV/dec) and long-term stability (error <4 mg/L). Furthermore, a denoising data processing algorithm (DDPA) was developed to further improve the sensor accuracy, reducing the S-ISE sensor error to only 1.2 mg/L after 50 days of real wastewater analysis. Based on the dynamic energy cost function and carbon footprint models, LTCM is expected to save 44.9% NH 4 + discharge, 12.8% energy consumption, and 26.7% greenhouse emission under normal operational conditions. This study unveils an innovative LTCM methodology by integrating advanced materials (anti-fouling layer coating) with sensor data processing (DDPA).

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Section: Resultsmentioning

confidence: 99%

Electrospraying Zwitterionic Copolymers as an Effective Biofouling Control for Accurate and Continuous Monitoring of Wastewater Dynamics in a Real-Time and Long-Term Manner

Huang

Qian

Wang

et al. 2022

Environ. Sci. Technol.

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“…Artificial saliva solution and ferrocyanide [47] pH, ROS measurement, and bacteria attachment and poration of Pseudomonas aeruginosa PBS, Fe(CN) 6 3-/4and Ru(NH 3 ) 6 3+/2+ [48] pH changes in dental-plaque-derived multi-species biofilm Artificial saliva and FcMeOH [49] Characterization of a 3D-printed hydrogel with Streptococcus mutans and Escherichia coli…”

Section: Streptococcus Mutansmentioning

confidence: 99%

“…The growth and attachment of bacteria are strongly influenced by the pH of their microenvironment [83]. To investigate the impact of local pH changes mediated by bacteria on the integrity of dental resin composite materials in the oral cavity, an SECM-based potentiometric pH microsensor was developed by Koley and co-workers [49]. The pH fluctuations of the biofilm formed by multiple strains of bacteria were monitored, revealing a decrease in pH at different stages of biofilm formation.…”

Section: Ph and Ros Measurementmentioning

confidence: 99%

A Brief Review of In Situ and Operando Electrochemical Analysis of Bacteria by Scanning Probes

Lin

Darvishi

2023

Biosensors

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Bacteria are similar to social organisms that engage in critical interactions with one another, forming spatially structured communities. Despite extensive research on the composition, structure, and communication of bacteria, the mechanisms behind their interactions and biofilm formation are not yet fully understood. To address this issue, scanning probe techniques such as atomic force microscopy (AFM), scanning electrochemical microscopy (SECM), scanning electrochemical cell microscopy (SECCM), and scanning ion-conductance microscopy (SICM) have been utilized to analyze bacteria. This review article focuses on summarizing the use of electrochemical scanning probes for investigating bacteria, including analysis of electroactive metabolites, enzymes, oxygen consumption, ion concentrations, pH values, biofilms, and quorum sensing molecules to provide a better understanding of bacterial interactions and communication. SECM has been combined with other techniques, such as AFM, inverted optical microscopy, SICM, and fluorescence microscopy. This allows a comprehensive study of the surfaces of bacteria while also providing more information on their metabolic activity. In general, the use of scanning probes for the detection of bacteria has shown great promise and has the potential to provide a powerful tool for the study of bacterial physiology and the detection of bacterial infections.

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“…Nguyen et al. (2022) developed a 300 μm diameter unique flexible pH wire sensor that is capable of measuring changes in local pH with high sensitivity for 14 days in a complex high‐protein bacterial growth medium while maintaining the highest hydrogen ion selectivity. This enabled real‐time monitoring of this highly dynamic interfacial chemical microenvironment as the dental plaque‐derived multispecies biofilm grew on various substrates.…”

Section: Electrochemical Sensors To Study the Biofilm–materials Inter...mentioning

confidence: 99%

“…Probing the chemical microenvironment at the biofilm-substrate interface is even more challenging, owing to the complexity of the matrix and the difficulty of developing an analytical method capable of tracking the microenvironment while maintaining a high selectivity for the primary analytes. Nguyen et al (2022) developed a 300 µm diameter unique flexible pH wire sensor that is capable of measuring changes in local pH with high sensitivity for 14 days in a complex high-protein bacterial growth medium while maintaining the highest hydrogen ion selectivity. This enabled real-time monitoring of this highly dynamic interfacial chemical microenvironment as the dental plaque-derived multispecies biofilm grew on various substrates.…”

Section: Electrochemical Sensors To Study the Biofilm-materials Inter...mentioning

confidence: 99%

Electrochemical sensors for oral biofilm–biomaterials interface characterization: A review

Koley

2022

Molecular Oral Microbiology

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Important processes related to the interaction of the oral microbiome with the tooth surface happen directly at the interface. For example, the chemical microenvironment that exists at the interface of microbial biofilms and the native tooth structure is directly involved in caries development. Consequentially, a critical understanding of this interface and its chemical microenvironment would provide novel avenues in caries prevention, including secondary caries that often occurs at the interface of the dental biofilm, tooth structure, and dental material. Electrochemical sensors are a unique quantitative tool and have the inherent advantages of miniaturization, stability, and selectivity. That makes the electrochemical sensors ideal tools for studying these critical biofilm microenvironments with high precision. This review highlights the development and applications of several novel electrochemical sensors such as pH, Ca 2+ , and hydrogen peroxide sensors as scanning electrochemical microscope probes in addition to flexible pH wire sensors for real-time bacterial biofilm-dental surface and dental materials interface studies. K E Y W O R D Sbiofilm, glass ionomer, oral microbiology (molecular) INTRODUCTIONElectrochemical sensors can be used to conduct research on various aspects of oral microbiology and dental materials. For instance, they can determine the amount of microbes in a sample, the pH, the amount of oxygen, and the amount of metals. In addition, they can be used to track the progress of dental materials research toward the development of more efficient anticaries properties.

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Real-time monitoring of the pH microenvironment at the interface of multispecies biofilm and dental composites

Cited by 6 publications

References 23 publications

Electrospraying Zwitterionic Copolymers as an Effective Biofouling Control for Accurate and Continuous Monitoring of Wastewater Dynamics in a Real-Time and Long-Term Manner

Electrospraying Zwitterionic Copolymers as an Effective Biofouling Control for Accurate and Continuous Monitoring of Wastewater Dynamics in a Real-Time and Long-Term Manner

A Brief Review of In Situ and Operando Electrochemical Analysis of Bacteria by Scanning Probes

Electrochemical sensors for oral biofilm–biomaterials interface characterization: A review

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