Real-time monitoring of the adherence of Streptococcus anginosus group bacteria to extracellular matrix decorin and biglycan proteoglycans in biofilm formation

Landrygan-Bakri, Janine; Wilson, Matthew; Williams, David Wynne; Lewis, Michael Alexander Oxenham; Waddington, Rachel J.

doi:10.1016/j.resmic.2012.07.006

Cited by 9 publications

(12 citation statements)

References 54 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Our previous studies have demonstrated that these SAG isolates favour binding to the dermatan sulphate conjugated decorin and biglycan and fibronectin, which are all predominant in soft tissues of PDL [22], lending these tissues in particular to bacterial biofilm formation. Whilst this study focussed on periodontal ligament, similar components are present in other soft connective tissues of the body and thus the ECM of these tissues could equally play a prominent role in the establishment of lung, brain and liver abscesses.…”

Section: Discussionmentioning

confidence: 95%

“…Adherence was lower for the chondroitin sulphate conjugated decorin and biglycan which is predominant in osseous tissues, suggesting a preference for colonisation of soft connective tissues [22]. Numerous streptococcal species have also been shown to bind to heparin sulphate, which are components of cell surface proteoglycans such as syndecan, via microbial surface cell recognition adhesion matrix molecules (MSCRAMMs) [23][24][25][26].…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Impact of bidirectional relationships between streptococcus anginosus group and host tissue matrix components on cellular activity: role in establishment of infection

Waddington¹,

Landrygan-Bakri²,

Wilson³

et al. 2013

Microbiol Discov

Self Cite

View full text Add to dashboard Cite

This paper investigates pathogenic mechanisms of the Streptococcus anginosus group (SAG) of bacteria which influence the biological activity of periodontal ligament (PDL) cells, endothelial cells and also how matrix proteins produced by these host cells influence bacterial virulence factors. Isolates of SAG species, designated S. anginosus, S. constellatus and S. intermedius, were derived from healthy commensal and clinical pathogenic infection sites. SAG culture supernatants contained multiple protein components which differed between isolates. All SAG supernatants increased cellular proliferation and decreased decorin synthesis and collagen assembly by PDL cells and reduced endothelial cell migration. SAG isolates responded differently to extracellular matrix (ECM) components synthesised by PDL cells, but there was an overall notable increase in hydrolytic enzyme activity and in the production of the cytotoxin intermedilysin by S. intermedius. Collectively, the results indicate that both commensal and pathogenic SAG isolates were capable of impairing the ability of PDL cells and endothelial cells to make functional vascularised tissue. Reduced decorin synthesis is likely to have a major impact on cell signalling, angiogenesis and matrix assembly. Furthermore, ECM components produced by PDL cells were differentially capable of moderately increasing SAG enzymic activity, leading to subtle ECM modifications. The impact this bidirectional effect has on the tissue remodelling process is discussed.

show abstract

Section: Discussionmentioning

confidence: 95%

Section: Introductionmentioning

confidence: 99%

Impact of bidirectional relationships between streptococcus anginosus group and host tissue matrix components on cellular activity: role in establishment of infection

Waddington¹,

Landrygan-Bakri²,

Wilson³

et al. 2013

Microbiol Discov

Self Cite

View full text Add to dashboard Cite

show abstract

“…SPR is being used to determine the binding mechanisms of bacterial species by testing their adhesion kinetics to various natural and synthetic materials. 28,29 The identification of bacterial species present in a fluid sample was very recently demonstrated by functionalizing the surface of a SPRi chip with antibodies. 30 While selectively identifying pathogens, this approach, forces the cells into specific orientations on the surface and does not allow them to assemble naturally and thus addresses a fundamentally different question from what is presented here.…”

Section: A Biofilm Analysismentioning

confidence: 99%

Using surface plasmon resonance imaging to study bacterial biofilms

et al. 2014

View full text Add to dashboard Cite

This paper describes the use of Surface Plasmon Resonance imaging (SPRi) as an emerging technique to study bacterial physiology in real-time without labels. The overwhelming majority of bacteria on earth exist in large multicellular communities known as biofilms. Biofilms are especially problematic because they facilitate the survival of pathogens, leading to chronic and recurring infections as well as costly industrial complications. Monitoring biofilm accumulation and removal is therefore critical in these and other applications. SPRi uniquely provides label-free, high-resolution images of biomass coverage on large channel surfaces up to 1 cm 2 in real time, which allow quantitative assessment of biofilm dynamics. The rapid imaging capabilities of this technique are particularly relevant for multicellular bacterial studies, as these cells can swim several body lengths per second and divide multiple times per hour. We present here the first application of SPRi to image Escherichia coli and Pseudomonas aeruginosa cells moving, attaching, and forming biofilms across a large surface. This is also the first time that biofilm removal has been visualized with SPRi, which has important implications for monitoring the biofouling and regeneration of fluidic systems. Initial images of the removal process show that the biofilm releases from the surface as a wave along the direction of the fluid flow. V C 2014 AIP Publishing LLC. [http://dx

show abstract

“…Decorin has been expressed in other cell types including rat glioma cells , human cervical cancer (HeLa) cells , WiDr colon carcinoma and A431 squamous carcinoma cells , CNS‐1 glioma cells , rat mesangial cells and HEK‐293 , but has not been characterized with respect to GAG decoration. Mouse decorin has been expressed as a CS PG in HeLa cells and found to contain 4‐sulfated CS disaccharides . Bovine decorin has also been expressed in E. coli and human decorin in Spodoptera frugiperda 21 (Sf21) cells .…”

Section: Extracellular Matrix Proteoglycansmentioning

confidence: 99%

“…Gene delivery of decorin in spontaneously hypertensive rats was found to inhibit hypertension‐induced cardiac fibrosis, remodelling and hypertrophy . Streptococcus anginosus group pathogens have been shown to bind to decorin via its GAG chain which has implications for dental abscesses .…”

Section: Extracellular Matrix Proteoglycansmentioning

confidence: 99%

Recombinant production of proteoglycans and their bioactive domains

Lord

Whitelock

2013

The FEBS Journal

View full text Add to dashboard Cite

Proteoglycans are ubiquitous dynamic molecules that are made up of a protein core to which specific linear glycosylation structures, known as glycosaminoglycans, have been covalently coupled. They have roles in many biological and pathological processes, which have been shown to be dependent on events involving the protein component and/or the glycosaminoglycan chains. This review focuses on the literature describing the recombinant expression and production of proteoglycans known to be present in the extracellular, cell surface and intracellular environments with an emphasis on how the structure of the molecule relates to its biological function and how this relationship has been explored using recombinant DNA technology for clinical applications.

show abstract

Real-time monitoring of the adherence of Streptococcus anginosus group bacteria to extracellular matrix decorin and biglycan proteoglycans in biofilm formation

Cited by 9 publications

References 54 publications

Impact of bidirectional relationships between streptococcus anginosus group and host tissue matrix components on cellular activity: role in establishment of infection

Impact of bidirectional relationships between streptococcus anginosus group and host tissue matrix components on cellular activity: role in establishment of infection

Using surface plasmon resonance imaging to study bacterial biofilms

Recombinant production of proteoglycans and their bioactive domains

Contact Info

Product

Resources

About