Real-time monitoring of ethane in human breath using mid-infrared cavity leak-out spectroscopy

Dahnke, Hannes; Kleine, D.; Hering, P.; Mürtz, Manfred

doi:10.1007/s003400100609

Cited by 83 publications

(47 citation statements)

References 2 publications

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“…3 These lasers are also used to detect nitric oxide for diagnosing asthma 4 . Finally, both quantum cascade and lead salt lasers have been used to detect ethane which is produced in lipid peroxidation and by some forms of cancer 12,13,14 . While all of these systems provide robust and highly sensitive detections of their analyte molecules, many operate in relatively narrow spectral regions.…”

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confidence: 99%

Cavity-enhanced optical frequency comb spectroscopy: application to human breath analysis

et al. 2008

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confidence: 99%

Cavity-enhanced optical frequency comb spectroscopy: application to human breath analysis

et al. 2008

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“…In earlier studies, our laboratory showed the successful application of the CALOS analyzer (6). This work demonstrated highest sensitivity [detection limit for ethane: 100 parts per trillion (ppt) in 5 s] and specificity in environmental and medical tasks.…”

Section: Technical Principles and Arrangements Of The Calos Spectrometermentioning

confidence: 99%

“…Our group has previously reported precise monitoring of various trace gases by using cavity leak-out spectroscopy (CALOS) (15), a continuous-wave variant of cavity ring-down spectroscopy, which was developed in the late 1980s (17). Recently, our laboratory has reported on the offline analysis of ethane in human breath, where the breath collection was performed with sample bags (6).…”

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confidence: 99%

Online recording of ethane traces in human breath via infrared laser spectroscopy

Basum

Dahnke

Halmer

et al. 2003

Journal of Applied Physiology

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A method is described for rapidly measuring the ethane concentration in exhaled human breath. Ethane is considered a volatile marker for lipid peroxidation. The breath samples are analyzed in real time during single exhalations by means of infrared cavity leak-out spectroscopy. This is an ultrasensitive laser-based method for the analysis of trace gases on the sub-parts per billion level. We demonstrate that this technique is capable of online quantifying of ethane traces in exhaled human breath down to 500 parts per trillion with a time resolution of better than 800 ms. This study includes what we believe to be the first measured expirograms for trace fractions of ethane. The expirograms were recorded after a controlled inhalation exposure to 1 part per million of ethane. The normalized slope of the alveolar plateau was determined, which shows a linear increase over the first breathing cycles and ends in a mean value between 0.21 and 0.39 liter Ϫ1 . The washout process was observed for a time period of 30 min and was modelled by a threefold exponential decay function, with decay times ranging from 12 to 24, 341 to 481, and 370 to 1,770 s. Our analyzer provides a promising noninvasive tool for online monitoring of the oxidative stress status. alveolar slope; breath analysis; cavity leak-out spectroscopy; lipid peroxidation; oxidative stress; washout AMONG THE VARIOUS VOLATILE hydrocarbons found in breath, the alkanes ethane (C 2 H 6 ) and pentane (C 5 H 12 ) have been extensively studied since they were identified as end products of the oxidative degradation (lipid peroxidation) of polyunsaturated fatty acids. The process of lipid peroxidation has gained interest as one of the important features of free radical-induced damage in biology and medicine (29). During peroxidation of omega-3 and omega-6 fatty acids, ethane and pentane, respectively, are formed and excreted via the lungs and thus can be detected in exhaled breath. Because lipid peroxidation is considered as the major, probably the only endogenous source of pentane and ethane, these volatile compounds may serve as specific markers for oxidative damage (8,25).The first report of breath ethane as a marker of in vivo lipid peroxidation was published by Riely et al. in 1974 (24). During the past decade, several studies provided evidence that ethane and pentane in exhaled air are useful markers of in vivo lipid peroxidation under certain clinical conditions (1-4, 8, 19, 20, 26, 27). Because pentane is metabolized in the liver, ethane is considered as the more reliable marker (10).Despite the growing number of reports on breath ethane and pentane, the development of rapid and sensitive analysis techniques for measurements of these markers in exhaled breath still remains a challenge. Ethane and pentane fractional concentrations in expired air are in the parts per billion (ppb; 1:10 9 ) range, which is below the detection limit of most analytical methods.The technique usually applied for quantifying these hydrocarbons in exhaled breath is gas chromatography (...

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“…Infrared absorption [1] based breath-content analysis using an optical integrated circuit has been studied widely for health monitoring [2,3,4] given the presence of various diseasemarkers [1,5,6] in breath-contents and the ppm-order sensing ability of infrared absorption. Additionally, the use of optical integrated circuits [7] is a promising solution for realizing the meter-long optical path length of regular gas cell [1,6,8,9] within a compact area considering the capability of realizing small radius of curvature. One of the critical issues with infrared sensing is the portion of light profiles outside of the waveguide (Γ air ), which can be used for sensing.…”

Section: Introductionmentioning

confidence: 99%

Proposal of multiple-slot silica high-mesa waveguide for infrared absorption

Chen

Hokazono

Tsujino

et al. 2013

IEICE Electron. Express

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We propose a multiple-slot silica high-mesa waveguide for infrared sensing considering the possibility of realizing a higher portion of an optical field out of the waveguide (which is defined as "Γ air "). Low Γ air leads to less light power being used for sensing, which limits sensing capabilities. The simulated results showed a high Γ air of 20.3% for a quadruple structure under λ = 1550 nm. A scattering loss of 0.06 dB/cm was predicted theoretically as well.

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Real-time monitoring of ethane in human breath using mid-infrared cavity leak-out spectroscopy

Cited by 83 publications

References 2 publications

Cavity-enhanced optical frequency comb spectroscopy: application to human breath analysis

Cavity-enhanced optical frequency comb spectroscopy: application to human breath analysis

Online recording of ethane traces in human breath via infrared laser spectroscopy

Proposal of multiple-slot silica high-mesa waveguide for infrared absorption

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