Real-Time Monitoring of DNA Manipulations Using Biosensor Technology

Effect of ionic strength on PNA-DNA hybridization on surfaces and in solution

“…This need is realized in optical evanescent wave biosensors 2-7 which offer a versatile approach for the detection and characterization of biological molecules. The most commonly employed biosensors are surface plasmon resonance ͑SPR͒ biosensors [8][9][10][11][12][13][14] with microfluidic sample handling. For hybridization studies and DNA detection, catcher molecules are immobilized to the sensor surface, and the binding process is monitored in real time after injection of a target analyte solution, detecting small local changes in refractive index at the sensor surface upon the biomolecular interaction.…”

A Highly Sensitive 27 MHz Quartz-Crystal Microbalance as a Device for Kinetic Measurements of Molecular Recognition on DNA Strands

“…[24][25][26] The basis of the BIAcore TM system is the optical phenomenon of surface plasmon resonance (SPR), in which the angle of incident beam required for SPR changes depending on binding amount of guest molecules due to the change of refractive index and other factors near the Au surface.…”

Dual Reactivity of Several Monoclonal Anti-nucleosome Autoantibodies for Double-stranded DNA and a Short Segment of Histone H3

“…Successive injec tions of ethanol amine (35 /xl) and 0.1 m HC1 (5 /xl) were then performed at a flow of 5 ¡iVmin. The protocol used to immobilize dsDNA was adapted from the procedure described by Nilsson et a l (22). The sensor chip was first treated by injecting successively 40 /xl of N-ethyl-N'-(3- % of inhibition F ig .…”