Real‐time monitoring of circulating tumor cell release during tumor manipulation using in vivo photoacoustic and fluorescent flow cytometry

Abstract

Standard medical procedures could trigger CTC release that may increase the risk of metastases. This finding suggests the guidance of cancer treatment and likely diagnosis by real-time monitoring of CTC dynamics followed by well-timed treatment to reduce CTCs in the blood. In vivo detection of intervention-amplified CTCs could be used for early diagnosis of a small tumor, which is undetectable with conventional methods.

Prevalence and number of circulating tumour cells and microemboli at diagnosis of advanced NSCLC

“…Manipulation of tumour in animal models (Juratli et al 2014) and in human lung cancer (Sawabata et al 2007;Yao et al 2014) is associated with delivery of CTC in the bloodstream. Since the same phenomenon might occur in lung tumours undergoing FNAB, in each patient 15 mL of blood was collected in two EDTA tubes (7.5 mL each) soon before CT-FNAB through the antecubital vein access routinely prepared before CT-FNAB.…”

Clinical studies monitoring circulating and disseminated tumor cells in gastrointestinal cancers

“…Secondly, useful methods for inoperable patients where there is no other possibility of obtaining information about the tumor character (which could be called 'real-time tumor biopsy') is another option. In addition, it seems that CTCs and DTCs could provide a very good source of information about the chemosensitivity and chemoresistance of the primary tumor and about distant sites of metastasis [12].…”

Cancer Cell Seeding Following Manipulation: An Ancient Concern According to the Traditional Iranian Medicine

“…Likewise, animal model studies in mice indicate that a biopsy of the breast tumor might increase the number of CTCs or risk of distant metastasis [16,17]. As well other manipulations such as pressure and laser treatment can increase the number of CTCs in nude mice with breast cancer [18,19].…”