Real-Time Monitoring of Circadian Rhythms in the Eye

Baba, Kenkichi; Tosini, Gianluca

doi:10.1007/978-1-0716-2593-4_37

Cited by 1 publication

(4 citation statements)

References 23 publications

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“…We found that bioluminescence rhythms in retinas preincubated with M199 medium gave highly variable rhythms which quickly attenuated. Conversely, in Figs 1, 2 and 3, the PEC and cornea explants still showed reasonably stable and consistent oscillations despite being cultured in M199 medium, as recommended by Baba and colleagues 7,11,12 . These effects could be explained by the fact that Neurobasal A medium contains a concentration of components optimized for culturing neurons 39 .…”

Section: Discussionmentioning

confidence: 79%

“…Neurobasal A medium preincubation increased the period lengths and altered the acrophases of corneal and PEC rhythms compared to the ones preincubated with M199. However, it was previously reported that recording PER2::LUC bioluminescence rhythms in retinal explants was possible with only M199 medium 7, 10, 28 . We found that bioluminescence rhythms in retinas preincubated with M199 medium gave highly variable rhythms which quickly attenuated.…”

Section: Discussionmentioning

confidence: 99%

“…The eye is considered as a peripheral clock with unique properties: It lies in direct contact with the principal timegiver (Zeitgeber) -light, and it is independent from the central clock, the SCN 4,5 . Our understanding of ocular clocks has been profoundly advanced by the development of real-time monitoring of bioluminescence of PER2::LUC knock-in mouse explants 6,7 . Circadian rhythms were found in numerous ocular tissues, including the retina [8][9][10] , cornea 6,11 , posterior eye cup (PEC) as a proxy for the retinal pigment epithelium (RPE) 12 and ciliary body 13,14 .…”

Section: Introductionmentioning

confidence: 99%

“…There is considerable variability in culturing protocols of PER2::LUC explants, with some groups using a 24h preincubation with Neurobasal A medium and continued culturing using other media 9,[17][18][19] , or without preincubation and culturing with M199 medium 7 or DMEM medium 6,16,20 . Little is known about the influence these conditions play in PER2::LUC bioluminescence recordings.…”

Section: Introductionmentioning

confidence: 99%

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Circadian Rhythms in Murine Ocular Tissues including Sclera are affected by Neurobasal A Medium Preincubation, Mouse Strain, but not Sex

Milićević,

Sandu,

Challet

et al. 2024

Preprint

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Purpose: Our understanding of ocular clocks has been profoundly advanced by the development of real-time recording of bioluminescence of PER2::LUC knock-in mouse explants. However, the effect of sex, mouse strain and culturing conditions on ocular clocks remains unknown. Here, we studied the role these variables play on PER2::LUC bioluminescence rhythms of ocular tissues: retinas, corneas and posterior eye cups (PEC). We also tested the hypothesis that the sclera contains a circadian oscillator by using scraped PEC as a proxy. Methods: Retinas, corneas, intact and scraped PECs were obtained from male and female PER2::LUC knock-in mice maintained on either a pigmented C57BL/6J or albino RjOrl:SWISS background. PER2::LUC bioluminescence rhythms in ocular tissues were measured using a Lumicycle. Results: We compared PER2::LUC bioluminescence rhythms between ocular tissues and found that all ocular tissues oscillated, including the scraped PEC, which was previously not known to oscillate. The rhythms in scraped PECs had lower amplitudes, longer periods and distinct acrophases compared to other ocular tissues. Ocular tissues of RjOrl:SWISS mice oscillated with higher amplitudes compared to the ones of C57BL/6J, with corneal rhythms being most affected by mouse strain. A 24h preincubation with Neurobasal A medium enhanced rhythms of ocular tissues, whereas sex differences were not detected for these rhythms. Conclusions: We discovered a novel oscillator in the sclera. PER2::LUC bioluminescence rhythms in murine ocular tissues are enhanced by Neurobasal A medium preincubation, mouse strain but not sex.

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Section: Discussionmentioning

confidence: 79%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Circadian Rhythms in Murine Ocular Tissues including Sclera are affected by Neurobasal A Medium Preincubation, Mouse Strain, but not Sex

Milićević,

Sandu,

Challet

et al. 2024

Preprint

View full text Add to dashboard Cite

show abstract

Real-Time Monitoring of Circadian Rhythms in the Eye

Cited by 1 publication

References 23 publications

Circadian Rhythms in Murine Ocular Tissues including Sclera are affected by Neurobasal A Medium Preincubation, Mouse Strain, but not Sex

Circadian Rhythms in Murine Ocular Tissues including Sclera are affected by Neurobasal A Medium Preincubation, Mouse Strain, but not Sex

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