Real-Time Monitoring and Analysis of Zebrafish Electrocardiogram with Anomaly Detection

Lenning, Michael; Fortunato, Joseph; Le, Tai; Clark, Isaac; Sherpa, Ang; Yi, Sangheon; Hofsteen, Peter; Thamilarasu, Geethapriya; Yang, Jingchun; Xu, Xiaolei; Han, Huy-Dung; Hsiai, Tzung K.; Cao, Hung

doi:10.3390/s18010061

Cited by 26 publications

(30 citation statements)

References 31 publications

(40 reference statements)

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“…Specifically, a finite impulse response (FIR) band-pass filter, cut off frequency set between 3 and 45 Hz, was used to filter raw ECG signals. The filtered signals were then passed through additional low-pass filter and high-pass filter to remove low and high frequency noise, and then we calculated its first derivatives 32 . We used window of moving average for smoothing and removing 50 Hz power and muscle activity noise (Supplemental Fig.…”

Section: Methodsmentioning

confidence: 99%

Development of a rapid and economic in vivo electrocardiogram platform for cardiovascular drug assay and electrophysiology research in adult zebrafish

Lin

Chou

Chen

et al. 2018

Sci Rep

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Zebrafish is a popular and favorable model organism for cardiovascular research, with an increasing number of studies implementing functional assays in the adult stage. For example, the application of electrocardiography (ECG) in adult zebrafish has emerged as an important tool for cardiac pathophysiology, toxicity, and chemical screen studies. However, few laboratories are able to perform such functional analyses due to the high cost and limited availability of a convenient in vivo ECG recording system. In this study, an inexpensive ECG recording platform and operation protocol that has been optimized for adult zebrafish ECG research was developed. The core hardware includes integration of a ready-to-use portable ECG kit with a set of custom-made needle electrode probes. A combined anesthetic formula of MS-222 and isoflurane was first tested to determine the optimal assay conditions to minimize the interference to zebrafish cardiac physiology under sedation. For demonstration, we treated wild-type zebrafish with different pharmacological agents known to affect cardiac rhythms in humans. Conserved electrophysiological responses to these drugs were induced in adult zebrafish and recorded in real time. This economic ECG platform has the potential to facilitate teaching and training in cardiac electrophysiology with adult zebrafish and to promote future translational applications in cardiovascular medicine.

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Section: Methodsmentioning

confidence: 99%

Development of a rapid and economic in vivo electrocardiogram platform for cardiovascular drug assay and electrophysiology research in adult zebrafish

Lin

Chou

Chen

et al. 2018

Sci Rep

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“…A series of ECG variables, including HR, P-wave amplitude, R-wave amplitude, and PP and RR intervals were calculated with an in-house Matlab code [28]. The root mean square of the successive differences (RMSSD) [29] was used to evaluate the short-period (60 seconds) beat-…”

Section: Analysis Of Zebrafish Ecg Recordingmentioning

confidence: 99%

Aging-associated sinus arrest and sick sinus syndrome in adult zebrafish

Yan¹,

Li²,

Bu³

et al. 2020

PLoS ONE

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Because of its powerful genetics, the adult zebrafish has been increasingly used for studying cardiovascular diseases. Considering its heart rate of~100 beats per minute at ambient temperature, which is very close to human, we assessed the use of this vertebrate animal for modeling heart rhythm disorders such as sinus arrest (SA) and sick sinus syndrome (SSS). We firstly optimized a protocol to measure electrocardiogram in adult zebrafish. We determined the location of the probes, implemented an open-chest microsurgery procedure, measured the effects of temperature, and determined appropriate anesthesia dose and time. We then proposed an PP interval of more than 1.5 seconds as an arbitrary criterion to define an SA episode in an adult fish at ambient temperature, based on comparison between the current definition of an SA episode in humans and our studies of candidate SA episodes in aged wild-type fish and Tg(SCN5A-D1275N) fish (a fish model for inherited SSS). With this criterion, a subpopulation of about 5% wild-type fish can be considered to have SA episodes, and this percentage significantly increases to about 25% in 3-year-old fish. In response to atropine, this subpopulation has both common SSS phenotypic traits that are shared with the Tg(SCN5A-D1275N) model, such as bradycardia; and unique SSS phenotypic traits, such as increased QRS/P ratio and chronotropic incompetence. In summary, this study defined baseline SA and SSS in adult zebrafish and underscored use of the zebrafish as an alternative model to study aging-associated SSS.

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“…It can reveal a decreased in heart rate concerning the human ether-a-go-go gene (hERG) channel blockade, allowing for drug testing. Moreover, zebrafish also provide an efficient genetic approach to reveal the genetic basis underlying molecular mechanisms of numerous heart diseases [51]. It could be a robust human cardiac electrophysiology model since its heart rate, and action potential morphology are similar, although some differences are apparent [52].…”

Section: Overview Of Adult Cardiac Rhythm Detectionmentioning

confidence: 99%

An Overview of Methods for Cardiac Rhythm Detection in Zebrafish

et al. 2020

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The heart is the most important muscular organ of the cardiovascular system, which pumps blood and circulates, supplying oxygen and nutrients to peripheral tissues. Zebrafish have been widely explored in cardiotoxicity research. For example, the zebrafish embryo has been used as a human heart model due to its body transparency, surviving several days without circulation, and facilitating mutant identification to recapitulate human diseases. On the other hand, adult zebrafish can exhibit the amazing regenerative heart muscle capacity, while adult mammalian hearts lack this potential. This review paper offers a brief description of the major methodologies used to detect zebrafish cardiac rhythm at both embryonic and adult stages. The dynamic pixel change method was mostly performed for the embryonic stage. Other techniques, such as kymography, laser confocal microscopy, artificial intelligence, and electrocardiography (ECG) have also been applied to study heartbeat in zebrafish embryos. Nevertheless, ECG is widely used for heartbeat detection in adult zebrafish since ECG waveforms’ similarity between zebrafish and humans is prominent. High-frequency ultrasound imaging (echocardiography) and modern electronic sensor tag also have been proposed. Despite the fact that each method has its benefits and limitations, it is proved that zebrafish have become a promising animal model for human cardiovascular disease, drug pharmaceutical, and toxicological research. Using those tools, we conclude that zebrafish behaviors as an excellent small animal model to perform real-time monitoring for the developmental heart process with transparent body appearance, to conduct the in vivo cardiovascular performance and gene function assays, as well as to perform high-throughput/high content drug screening.

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Real-Time Monitoring and Analysis of Zebrafish Electrocardiogram with Anomaly Detection

Cited by 26 publications

References 31 publications

Development of a rapid and economic in vivo electrocardiogram platform for cardiovascular drug assay and electrophysiology research in adult zebrafish

Development of a rapid and economic in vivo electrocardiogram platform for cardiovascular drug assay and electrophysiology research in adult zebrafish

Aging-associated sinus arrest and sick sinus syndrome in adult zebrafish

An Overview of Methods for Cardiac Rhythm Detection in Zebrafish

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