Real-time modelling of dangerous wind speed gusts and thermal comfort conditions in campus of Moscow State University (Moscow, Russian Federation)

Konstantinov, Pavel; Perkhurova, A. A.; Nikolaeva, E. V.; Bukin, S. S.; Varentsov, Mikhail

doi:10.5194/egusphere-egu2020-12780

Cited by 1 publication

(2 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Therefore, when studying the structure of macroinvertebrate communities, emphasis was placed on the number of reads per OTU, as well as the occurrence of OTUs in samples. To understand the extent to which the molecular genetics me thod using highthroughput sequencing technologies is applicable to the study of macro invertebrate communities, the same approach was used for comparison as in ecological studies of water bodies based on classical hydrobiological methods (Brotskaya, Zenkevich, 1939;Begon et al, 1986;Konstantinov, 1986).…”

Section: Discussionmentioning

confidence: 99%

“…Communities of macroinvertebrates were identified by clustering the OTUs of the species rank and their COI reads by the Ward method; the Euclidean metric was used as a distance measure. Dominants and subdominants in the communities were determined by descending ranking of the modified density index (occurrence of OTUs in samples and their rela tive abundance = reads, in %) (Brotskaya, Zenkevich, 1939;Konstantinov, 1986). The communities of macroinvertebrates were named according to the dominant species with the highest density index.…”

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

A study of macroinvertebrate communities in Bolshiye Koty Bay of Lake Baikal using DNA metabarcoding

Kravtsova,

Peretolchina,

Triboy

et al. 2023

Vestn. VOGiS

View full text Add to dashboard Cite

The diversity of macroinvertebrates, the structure of their communities in Bolshiye Koty Bay (Lake Baikal) was studied by a DNA metabarcoding approach using an Illumina MiSeq system. Internal primer mlCOIintF in combination with jgHCO2198 of the Folmer fragment of the COI gene were used for macroinvertebrate metabarcoding. A total of 118009 reads of the COI gene fragment (at least 313 bp in length) were obtained. The correlation of the Spearman coefficient (S = 0.6, p < 0.05) with the abundance of macroinvertebrates in the samples before DNA extraction showed that the number of reads can serve as an indirect characteristic of the abundance of a species (operational taxonomic unit, OTU). 115 OTUs belonging to the higher taxa of macroinvertebrates were identified: Porifera, 1; Platyhelminthes, 3; Annelida, 38; Arthropoda, 55; Mollusca, 18. At a high level of resolution (with homology with GenBank reference sequences ≥ 95 %, coverage ≥ 90 %), 46 taxa of macroinvertebrates comprising three communities were registered: one dominated by molluscs (Choanomphalus conf. maacki) and two dominated by chironomids (Orthocladius grega rius Linev., Sergentia baicalensis Tshern.). Communities are characterized by low species diversity according to Shannon (from 0.7 to 1.2 bits), high concentration of dominance according to Simpson (from 0.5 to 0.7) and low evenness according to Pielou (from 0.3 to 0.4). Dominants and subdominants in the communities account for 91 to 96 % of COI gene fragment reads. The spatial distribution of the dominant species identified in the communities is influenced by the geomorphological features of the bottom and the composition of sediments in the area studied. The approach proposed for studying the structure of macroinvertebrate communities based on DNA metabarcoding and next generation sequencing can be recommended for express assessment of the state of aquatic ecosystems in the monitoring.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%