Real‐time measurement of fluorescence spectra from single airborne biological particles

Hill, Steven C.; Pinnick, Ronald G.; Niles, Stanley; Pan, Yong‐Le; Holler, Stephen; Chang, Richard K.; Bottiger, Jerold R.; Chen, Bean T.; Orr, Chun-Sing; Feather, Greg

doi:10.1002/(sici)1520-6521(1999)3:4/5<221::aid-fact2>3.3.co;2-z

Cited by 60 publications

(88 citation statements)

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“…B. subtilis var. niger (B. atrophaeus) spores are often used as the surrogate of anthrax causing agent Bacillus anthracis (Hill et al 1999). However, the sporulation state of the cells was not verified here.…”

Section: Preparation Of Microorganismsmentioning

confidence: 99%

A novel method for measuring the charge distribution of airborne microbes

2010

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A novel method for measuring bioaerosol charge distribution was investigated using electrostatic sampling and quantitative polymerase chain reaction (qPCR). Here, Bacillus subtilis var niger and Pseudomonas fluorescens were aerosolized and precipitated into different regions of two 96-well plates placed inside an electrostatic sampler. The concentrations of negatively charged bacteria in the air samples collected were quantified using qPCR, and the relevant bioaerosol charge levels were calculated using an equation developed in this study. Experimental results showed that B. subtilis var niger bioaerosols had wider charge distribution than P. fluorescens. For B. subtilis var niger, 93% of them carried a charge level of 13-42 units, 2% carried a charge level of 8-13 or lower, and 5% carried a charge level of 42-195 or higher. While for P. fluorescens, 99% of P. fluorescens aerosols carried a charge level of 14-34 elementary units. The indicative charge levels of bioaerosols collected at a certain point in the electrostatic sampling line largely depends on the particle size, bacterial species, electrostatic field strength, and the sampling flow rates for a given electrostatic sampler. In general, the trends for the charge levels here were similar to those obtained in previous studies. Use of qPCR technology in this study can distinguish between biological and non-biological particles collected. Thus, this avoided counting the non-biological particles in traditional bioaerosol charge measurements in which an optical particle counter is used. The developed technique here can be also used to analyze the charge levels for other types of bioaerosols.

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Section: Preparation Of Microorganismsmentioning

confidence: 99%

A novel method for measuring the charge distribution of airborne microbes

2010

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“…The physical aerosols included polydisperse oleic acid liquid droplets (d a = 0.5-5 m) and monodisperse polystyrene latex (Meldrum, O'Rourke, Boyer-Pfersdorf, & Stetzenbach, 1993;Madelin, 1994;Horner, Helbling, Salvaggio, & Lehrer, 1995;ACGIH, 1999). The BG spores are also commonly used as a simulant of Bacillus anthracis and several other biological agents (Johnson, Martin, & Resnick, 1994;Franz, Parrott, & Takafuji, 1997;Hill et al, 1999). In addition, for some selected experiments, Penicillium brevicompactum [fungal spores of d a = 2.3 m ( g = 1.1), CBS 480-84] and Penicillium melinii [fungal spores of d a = 3.0 m ( g = 1.1), isolated from a moldy building] were used as test microorganisms.…”

Section: Test Aerosolsmentioning

confidence: 99%

Collection of airborne spores by circular single-stage impactors with small jet-to-plate distance

Grinshpun

Mainelis

Trunov

et al. 2005

Journal of Aerosol Science

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“…Also here distinct differences in the spectra can be seen. Comparable fluorescence spectra had been found by Hill et al [25] before. They measured fluorescence of small aggregates of bacteria and used a pulsed laser at 266 nm.…”

Section: Resultsmentioning

confidence: 99%

“…The fluorescence spectra of fungal spores and b. subtilis vegetative cells e.g. are nearly identical at 266 nm excitation, but there is a distinct difference in the spectra, if excitation occurs at 351 nm [25]. Fluorescence seems to be an appropriate tool for the detection and identification of biological aerosols.…”

Section: Introductionmentioning

confidence: 99%