Real-Time, label-free monitoring of tumor antigen and serum antibody interactions

Campagnolo, Christine; Meyers, Katherine J.; Ryan, Thomas E.; Atkinson, Robert; Chen, Yao‐Tseng; Scanlan, Matt J.; Ritter, Gerd; Old, Lloyd J.; Batt, Carl A.

doi:10.1016/j.jbbm.2004.05.006

Cited by 76 publications

(42 citation statements)

References 23 publications

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“…All 170 samples (100%) analyzed by SPR tested positive. These results suggest that protein chip based on SPR was 10 times as sensitive as ELISA, which supports a previous report suggesting that the assay protein chip based on SPR is much more sensitive than the standard ELISA [2,7]. Figure 2 shows a sensorgram of one sample (No.…”

supporting

confidence: 86%

“…Until now, most studies using the SPR assay detected the antigens or pathogen directly such as the foot and mouth disease virus [5], vaccinia virus, poliovirus, cowpea mosaic virus and tobacco mosaic virus [4] and Legionella pneumophila [13]. On the other hand, detection of the Ab using the SPR system has only rarely been carried out except for detecting tumor antigens [2]. Compared with ELISA, the protein chip based on SPR appears to be a fast (total 1.5 hr), valuable tool in the serodiagnosis of CSFV infection or Ab titers after vaccination.…”

mentioning

confidence: 99%

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Serodiagnostic Comparison between Two Methods, ELISA and Surface Plasmon Resonance for the Detection of Antibodies of Classical Swine Fever

Cho

Park

2006

The Journal of Veterinary Medical Science

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ABSTRACT. A protein chip based on surface plasmon resonance (SPR) was develpoped to measure the antibody (Ab) titers of classical swine fever virus (CSFV) using the recombinant gp55 protein as an antigen. The diagnostic potential of this SPR assay for detecting the Ab titers to CSFV gp55 was compared that of the enzyme-linked immunosorbent assay (ELISA) using 170 serum samples from 14 pig farms. The SPR assay was highly specific and sensitive, and there were no cross-reactions detected. There was a strong positive correlation between the SPR and ELISA titers (n=170, r=0.869, p<0.01). Therefore, the SPR label-free method is a valuable tool in the serodiagnosis of CSFV infection and determining Ab titers after vaccination. KEY WORDS: classical swine fever, protein chip, surface plasmon resonance.

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confidence: 86%

mentioning

confidence: 99%

Serodiagnostic Comparison between Two Methods, ELISA and Surface Plasmon Resonance for the Detection of Antibodies of Classical Swine Fever

Cho

Park

2006

The Journal of Veterinary Medical Science

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“…Similarly, detection methods based on specific recognition of extracellular (cell surface) biomarkers such as histopathology (4), bioimaging (5), antibody arrays require prior knowledge of biomarkers on cell surfaces. Observation of overexpressed antigens (6) on tumor cells using antibody-based platforms have been explored using ELISA (7), surface plasmon resonance (8,9), nanoparticles (10-13), microcantilevers (14), carbon nanotubes (15,16), and expression microarrays (17). Antibody arrays provide an effective but complex approach for cancer detection, diagnosis and prognosis (18), however, there is no single marker or a combination of biomarkers that has sufficient sensitivity and specificity to differentiate between normal, cancerous, and metastatic cell types (19).…”

mentioning

confidence: 99%

Detection and differentiation of normal, cancerous, and metastatic cells using nanoparticle-polymer sensor arrays

Bajaj¹,

Miranda²,

Kim

et al. 2009

Proc. Natl. Acad. Sci. U.S.A.

284

253

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Rapid and effective differentiation between normal and cancer cells is an important challenge for the diagnosis and treatment of tumors. Here, we describe an array-based system for identification of normal and cancer cells based on a ''chemical nose/tongue'' approach that exploits subtle changes in the physicochemical nature of different cell surfaces. Their differential interactions with functionalized nanoparticles are transduced through displacement of a multivalent polymer fluorophore that is quenched when bound to the particle and fluorescent after release. Using this sensing strategy we can rapidly (minutes/seconds) and effectively distinguish (i) different cell types; (ii) normal, cancerous and metastatic human breast cells; and (iii) isogenic normal, cancerous and metastatic murine epithelial cell lines.fluorescence ͉ gold nanoparticle ͉ sensor ͉ conjugated polymer

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“…Au excited at red wavelengths) as its intensity decays exponentially. Therefore, the sensing mechanism of SPR spectroscopy is based on the measurement of small changes in the refractive index in the vicinity of the surface of a conductive thin film [105,107,108,109].…”

Section: Electrochemical Surface-plasmon Resonance (Ec-spr)mentioning

confidence: 99%

Electrochemical Biosensors - Sensor Principles and Architectures

Grieshaber

MacKenzie

Vörös

et al. 2008

Sensors

819

632

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Quantification of biological or biochemical processes are of utmost importance for medical, biological and biotechnological applications. However, converting the biological information to an easily processed electronic signal is challenging due to the complexity of connecting an electronic device directly to a biological environment. Electrochemical biosensors provide an attractive means to analyze the content of a biological sample due to the direct conversion of a biological event to an electronic signal. Over the past decades several sensing concepts and related devices have been developed. In this review, the most common traditional techniques, such as cyclic voltammetry, chronoamperometry, chronopotentiometry, impedance spectroscopy, and various field-effect transistor based methods are presented along with selected promising novel approaches, such as nanowire or magnetic nanoparticle-based biosensing. Additional measurement techniques, which have been shown useful in combination with electrochemical detection, are also summarized, such as the electrochemical versions of surface plasmon resonance, optical waveguide lightmode spectroscopy, ellipsometry, quartz crystal microbalance, and scanning probe microscopy. The signal transduction and the general performance of electrochemical sensors are often determined by the surface architectures that connect the sensing element to the biological sample at the nanometer scale. The most common surface modification techniques, the various electrochemical transduction mechanisms, and the choice of the recognition receptor molecules all influence the ultimate sensitivity of the sensor. New nanotechnology-based approaches, such as the use of engineered ion-channels in lipid bilayers, the encapsulation of enzymes into vesicles, polymersomes, or polyelectrolyte capsules provide additional possibilities for signal amplification. In particular, this review highlights the importance of the precise control over the delicate interplay between surface nano-architectures, surface functionalization and the chosen sensor transducer principle, as well as the usefulness of complementary characterization tools to interpret and to optimize the sensor response.

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Real-Time, label-free monitoring of tumor antigen and serum antibody interactions

Cited by 76 publications

References 23 publications

Serodiagnostic Comparison between Two Methods, ELISA and Surface Plasmon Resonance for the Detection of Antibodies of Classical Swine Fever

Serodiagnostic Comparison between Two Methods, ELISA and Surface Plasmon Resonance for the Detection of Antibodies of Classical Swine Fever

Detection and differentiation of normal, cancerous, and metastatic cells using nanoparticle-polymer sensor arrays

Electrochemical Biosensors - Sensor Principles and Architectures

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