Real-time, in vivo confocal reflectance microscopy of basal cell carcinoma

González, Salvador; Tannous, Zeina

doi:10.1067/mjd.2002.124690

Cited by 277 publications

(260 citation statements)

References 11 publications

Supporting

Mentioning

254

Contrasting

Order By: Relevance

“…[9][10][11][12][13][14][15][16][17] Some RCSLM features of melanocytic lesions have been recently identified. [10][11][12][13] Studying four melanomas, two lentigo maligna melanomas and 34 melanocytic nevi, distinct cytological features were found at the RCSLM observation.…”

mentioning

confidence: 99%

In vivo assessment of melanocytic nests in nevi and melanomas by reflectance confocal microscopy

2005

View full text Add to dashboard Cite

In vivo reflectance confocal microscopy is a novel technique for the noninvasive study and diagnosis of the skin. The aim of this study was to describe and characterize the cytological and architectural aspects of cell clusters in melanocytic lesions observed by confocal microscopy, and to correlate them with routine histopathology. A total of 55 melanocytic lesions comprising 20 melanomas, 25 acquired nevi and 10 Spitz nevi were studied by means of reflectance confocal microscopy, dermoscopy and routine histopathology. Three different types of cell clusters at confocal microscopy observation (dense, sparse cell and cerebriform clusters) were identified and correlated with histopathology. Dense clusters appeared characteristic for benign lesions, although present in 13 out of 20 melanomas. Sparse cell clusters were more frequently observable in melanomas, but also sporadically present in one Spitz nevus. Moreover, cerebriform clusters were exclusively observed in five out of 20 melanomas. Confocal microscopy allowed the in vivo characterization of aspects of melanocytic nests and their exact correlation with histopathology. Keywords: histopathology; melanoma; near-infrared confocal microscopy; Spitz neviIn vivo reflectance-mode confocal scanning laser microscopy (RCSLM) enables the instantaneous visualization of skin structures at a quasihistopathologic resolution, 1,2 representing a noninvasive approach for the in vivo study of normal skin, 1-4 inflammatory skin diseases 5-8 and skin tumours. [9][10][11][12][13][14][15][16][17] Some RCSLM features of melanocytic lesions have been recently identified. 10-13 Studying four melanomas, two lentigo maligna melanomas and 34 melanocytic nevi, distinct cytological features were found at the RCSLM observation. 10 The presence of cells clustered into nests was reported in melanocytic nevi 9,10 and in lentigo maligna, 13 but not described in details.Differential diagnosis between melanocytic lesions depends on the histopathological analysis of numerous aspects, including the description of cytological and architectural features of melanocytic nests. 18 Whereas shape and size regularity of the nests, composed by cohesive cuboidal cells, is suggestive of benign lesions, confluent nests with atypical cells, sometimes presenting cellular discohesion, are typical of malignant ones. Moreover, Spitz nevi are characterized by large nests not tending to become confluent, sometimes presenting peculiar artefactual clefts above the nests at the dermal-epidermal junction. 19 The aim of this study was to describe and characterize the cytological and architectural aspects of cell clusters observed at the RCSLM examination of melanocytic lesions, and to correlate them with histopathology. Materials and methods SubjectsThis study included 55 melanocytic lesions comprising 20 melanomas (mean Breslow's thickness 0.81 mm), 25 acquired melanocytic nevi and 10 Spitz nevi. Lesions were selected for the presence of cellular clusters with well-defined borders in the superficial dermis at RCSLM obs...

show abstract

mentioning

confidence: 99%

In vivo assessment of melanocytic nests in nevi and melanomas by reflectance confocal microscopy

2005

View full text Add to dashboard Cite

show abstract

“…Similarly, spectroscopy and imaging of polarized light have been used to study epithelial tissues [8,9], including those involved in skin pathology [10]. Confocal reflectance microscopy provides high resolution images of tissue morphology and has been used to investigate skin lesions [11,12], but this technique does not provide functional information about the tissue. Optical coherence tomography (OCT) [13] allows imaging at greater depths within tissue but, again, does not normally supply functional information.…”

Section: Introductionmentioning

confidence: 99%

In vivo measurements of diffuse reflectance and time‐resolved autofluorescence emission spectra of basal cell carcinomas

Thompson

Coda

Sørensen

et al. 2012

Journal of Biophotonics

View full text Add to dashboard Cite

Journal of BIOPHOTONICSWe present a clinical investigation of diffuse reflectance and time-resolved autofluorescence spectra of skin cancer with an emphasis on basal cell carcinoma. A total of 25 patients were measured using a compact steady-state diffuse reflectance/fluorescence spectrometer and a fibre-optic-coupled multispectral time-resolved spectrofluorometer. Measurements were performed in vivo prior to surgical excision of the investigated region. Singular value decomposition was used to reduce the dimensionality of steady state diffuse reflectance and fluorescence spectra. Linear discriminant analysis was then applied to the measurements of basal cell carcinomas (BCCs) and used to predict the tissue disease state with a leave-one-out methodology. This approach was able to correctly diagnose 87% of the BCCs. With 445 nm excitation a decrease in the spectrally averaged fluorescence lifetime was observed between normal tissue and BCC lesions with a mean value of 886 ps. Furthermore, the fluorescence lifetime for BCCs was lower than that of the surrounding healthy tissue in all cases and statistical analysis of the data revealed that this decrease was significant (p ¼ 0.002).Schematic diagrams of the two spectrometers showing the steady state spectrometer measurement head (top) and the optical layout of the time-resolved system (bottom).

show abstract

“…The high image contrast results from a difference between strong light absorption of nucleic acids and weak absorption of protein, which reaches the maximum at a wavelength of 250 nm. In addition to UV-PAM, several other modern optical microscopy technologies have been explored for in vivo imaging of unstained cell nuclei, such as reflectance confocal microscopy [8][9][10][11], multiphoton microscopy [12][13][14], and third-harmonic generation microscopy [15,16], However, reflectance confocal microscopy is difficult to provide specific image contrast for nuclei [8], multiphoton microscopy produces in vivo images of cell nuclei with negative contrast [13], and third harmonic generation microscopy generates nuclear images with low contrast [15].…”

Section: Discussionmentioning

confidence: 99%

In vivo imaging of cell nuclei by photoacoustic microscopy without staining

et al. 2012

View full text Add to dashboard Cite

Ultraviolet photoacoustic microscopy (UVPAM) can image cell nuclei in vivo with high contrast and resolution noninvasively without staining. Here, we used UV light at wavelengths of 210-310 nm for excitation of DNA and RNA to produce photoacoustic waves. We applied the UVPAM to in vivo imaging of cell nuclei in mouse skin, and obtained UVPAM images of the unstained cell nuclei at wavelengths of 245-282 nm as ultrasound gel was used for acoustic coupling. The largest ratio of contrast to noise was found for the images of cell nuclei at a 250 nm wavelength.

show abstract

Real-time, in vivo confocal reflectance microscopy of basal cell carcinoma

Cited by 277 publications

References 11 publications

In vivo assessment of melanocytic nests in nevi and melanomas by reflectance confocal microscopy

In vivo assessment of melanocytic nests in nevi and melanomas by reflectance confocal microscopy

In vivo measurements of diffuse reflectance and time‐resolved autofluorescence emission spectra of basal cell carcinomas

In vivo imaging of cell nuclei by photoacoustic microscopy without staining

Contact Info

Product

Resources

About