Real time evaluation of tissue optical properties during thermal ablation of <i>ex vivo</i> liver tissues

Nagarajan, Vivek Krishna; Gogineni, Venkateswara Rao; White, Sarah B.; Yu, Bing

doi:10.1080/02656736.2018.1488278

Cited by 18 publications

(15 citation statements)

References 35 publications

(39 reference statements)

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“…In our previous studies , we showed that the relative changes in µ a and

μ_{s}^{'}

of ex vivo porcine liver tissues follow a sigmoidal curve, which correlates well with the thermal damage calculated using the Arrhenius damage model. In this paper, relative changes in µ a and

μ_{s}^{'}

of porcine liver tissues are associated with histopathologically assessed thermal damage.…”

Section: Introductionsupporting

confidence: 77%

“…Future studies may be performed in a water heating bath. In our previous papers , we have shown that the relative changes in µ a and

μ_{s}^{'}

have a better contrast than the absolute µ a and

μ_{s}^{'}

values. The relative changes in µ a for Groups 3 and 4 were significantly different from each other ( P = 0.003) (Fig.…”

Section: Discussionmentioning

confidence: 90%

“…The liver tissue samples in each group were exposed to different thermal dosages. In general, the optical tissue properties increased for all tissue samples during heating . Therefore, we explored the feasibility of using µ a and

μ_{s}^{'}

to identify the tissue statuses at different stages of heating.…”

Section: Discussionmentioning

confidence: 99%

“…The integrated DRS system used in this paper has been illustrated in our previous paper . In brief, the DRS system consists of a homemade needle‐based fiber‐optic probe, two visible‐light spectrometers (Avantes BV, Apeldoorn, The Netherlands) with a white light‐emitting diode (LED), one near‐infrared (NIR) spectrometer with an 820 nm LED, and a laptop computer loaded with custom software.…”

Section: Methodsmentioning

confidence: 99%

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Association of Liver Tissue Optical Properties and Thermal Damage

Nagarajan

Ward

2020

Lasers Surg Med

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Background and Objectives Complete thermocoagulation of tumors is vital to minimize the risk of local tumor recurrence after a thermal ablation. Histological assessments are not real‐time and require experienced pathologists to grade the thermal damage (histopathology) [Correction added on 21 January, 2020 after first online publication: After thermal damage in the preceding sentence, (histopathology) was added]. Real‐time assessment of thermal tissue damage during an ablation is necessary to achieve optimal tumor ablation. In our previous studies, we found that continuous monitoring of the wavelength‐averaged (435–630 nm) tissue absorption coefficient (µa) and the reduced scattering coefficient ( μ s ′) during heating of a porcine liver at 100°C follows a sigmoidal growth curve. Therefore, we concluded that increases in the tissue µa and μ s ′ during thermocoagulation were correlated with true thermal damage. The goal of this study was to determine if increases in the tissue µa and μ s ′ during thermocoagulation are correlated with true thermal damage. Study Design/Materials and Methods In this paper, continuously measured values of µa and μ s ′ during heating of the porcine liver tissue were compared with the histology‐assessed thermal damage scores at four different temperature points (37°C, 55°C, 65°C, and 75°C). Results The damage scores for the tissues in Group 3 (65°C) and Group 4 (75°C) were significantly different from each other and from the other groups. The damage scores were not significantly different between Group 1 (37°C) and Group 2 (55°C). Conclusion The results indicate that relative changes in µa and μ s ′ can be used to classify thermal damage (histopathology) scores with an overall accuracy of 72.5% up to 75°C. [Correction added on 21 January, 2020 after first online publication: After thermal damage in the preceding sentence, (histopathology) was added]. Lasers Surg. Med. © 2019 Wiley Periodicals, Inc.

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“…In our previous studies , we showed that the relative changes in µ a and

μ_{s}^{'}

of ex vivo porcine liver tissues follow a sigmoidal curve, which correlates well with the thermal damage calculated using the Arrhenius damage model. In this paper, relative changes in µ a and

μ_{s}^{'}

of porcine liver tissues are associated with histopathologically assessed thermal damage.…”

Section: Introductionsupporting

confidence: 77%

“…Future studies may be performed in a water heating bath. In our previous papers , we have shown that the relative changes in µ a and

μ_{s}^{'}

have a better contrast than the absolute µ a and

μ_{s}^{'}

values. The relative changes in µ a for Groups 3 and 4 were significantly different from each other ( P = 0.003) (Fig.…”

Section: Discussionmentioning

confidence: 90%

μ_{s}^{'}

to identify the tissue statuses at different stages of heating.…”

Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

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Association of Liver Tissue Optical Properties and Thermal Damage

Nagarajan

Ward

2020

Lasers Surg Med

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“…Hepatocellular carcinoma (HCC) is the sixth most common malignancy globally and the third most common cause of cancer-related death [1][2][3]. Radiofrequency ablation (RFA), a type of hyperthermia treatment, has been accepted as a curative therapy alternative to surgical resection and liver transplantation for small HCC due to such advantages as minimal invasiveness, safety, repeatability and shorter hospitalization time [4].…”

Section: Introductionmentioning

confidence: 99%

Targeting autophagy enhances heat stress-induced apoptosis via the ATP-AMPK-mTOR axis for hepatocellular carcinoma

Jiang

Chen

et al. 2019

International Journal of Hyperthermia

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Purpose: Radiofrequency ablation (RFA) is widely accepted as a curative treatment for small hepatocellular carcinoma (HCC). However, insufficient RFA (IRFA) can lead to rapid local recurrence. The underlying mechanisms remain poorly understood. This study aimed to elucidate the role and regulatory mechanisms of autophagy in the recurrence of HCC after IRFA. Materials and methods: SMMC7721 and Huh7 cells were exposed to sublethal heat stress to stimulate the transition zone of IRFA treatment. The levels of autophagy were measured by western blot, immunofluorescence and transmission electron microscopy. Functional assays, such as CCK-8, EdU incorporation and flow cytometry, were performed to determine the role of heat-induced autophagy. The involved signaling pathways were explored by western blot. Finally, the antitumor effects of chloroquine (CQ) on heat-treated HCC cells were evaluated via an in vivo xenograft tumor model. Results: Sublethal heat stress induced autophagy in a temperature-and time-dependent manner in HCC cells. Furthermore, the inhibition of autophagy by CQ or siRNA targeting the autophagy-related genes Beclin-1 and Atg5 enhanced heat-induced apoptosis. The combination of CQ and heat treatment significantly suppressed tumor growth both in vitro and in vivo. Mechanistically, we reported for the first time that the ATP-AMPK-mTOR signaling pathway was involved in heat-induced autophagy. Conclusions: Heat stress induced protective autophagy against heat-induced apoptosis in HCC via the ATP-AMPK-mTOR axis, suggesting that targeting autophagy may be a promising strategy for improving the efficacy of RFA treatment for HCC.

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Radiofrequency impedance monitoring of the biological tissues under local heating by optical radiation

Коваленко

Sovin

Malikova

et al. 2022

Journal of Biophotonics

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Development of methods for simultaneous control of state of biological tissues during optical treatment is the important tasks in laser surgery. We introduce a novel approach for the monitoring of the state of biological tissues in the process of its local heating by optical radiation. It is based on measurements of the electrical radiofrequency impedance kinetics of the sample during irradiation. The obtained data are processed using interconnected mathematical modeling of corresponding thermodynamic, optical and electrical problems. Experimental applications of this approach, represented in the paper, reveal its high sensitivity, repeatability and consistency with the model. The introduced method can be used for the selection and optimization of radiation parameters of medical laser sources as an alternative or an addition to histological techniques. Radiofrequency impedance measurement can be used directly in the course of surgical operations for monitoring the treated tissues state, including its temperature and degree of damage.

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Real time evaluation of tissue optical properties during thermal ablation of ex vivo liver tissues

Cited by 18 publications

References 35 publications

Association of Liver Tissue Optical Properties and Thermal Damage

Association of Liver Tissue Optical Properties and Thermal Damage

Targeting autophagy enhances heat stress-induced apoptosis via the ATP-AMPK-mTOR axis for hepatocellular carcinoma

Radiofrequency impedance monitoring of the biological tissues under local heating by optical radiation

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